Amino acid stable N isotope estimations reveal uniform diazotrophic contributions across zooplankton size fractions in the subtropical N Atlantic

• Main Authors: Mompeán-de-la-Rosa, M.C. (María del Carmen), Bode, A. (Antonio), Gier, E., McCarthy, M.D.
• Format: Conference Object
• Language: English
• Published: Centro Oceanográfico de A Coruña 2016
• Subjects: Stable isotopes; δ15N; Plankton; Compound-specific; N fixation; Amino acids; Diazotrophy; Trophic position; Subtropical; Bergen; Norway; North Atlantic
• Online Access: http://hdl.handle.net/10508/10153; http://hdl.handle.net/10261/316661

presentacion Natural abundance of stable N isotopes (d15N) in both individual amino acids and bulk organic matter of size-fractionated plankton samples were compared to analyze the differential impact of nitrogen fixation through the food web, in a transect across the subtropical North Atlantic. Low d15N values in the central and western regions were consistent with the prevalence of nitrogen fixation, while maximum d15N values tracked the influence of West African upwelling in the eastern zone. Compound-specific amino acid isotope data (CSI-AA) revealed relatively low variability in the impact of diazotrophic nitrogen within the different plankton size fractions, while d15N of bulk organic matter showed higher variability with size. Moreover, CSI-AA results also indicated a greater importance of diazotrophy than suggested by bulk d15N values. Trophic position estimates using CSI-AA showed the expected general increase with mean plankton size class and varied in a relatively narrow range (1.8 to 2.5), with the lowest values in the central zone. Using isotopic δ15N values of individual amino acids (in particular Phe and Thr), as well as reconstructed total protein d15N values, a set of new relationships with bulk plankton d15N was determined, aimed to improve the use CSI-AA data in tracing direct plankton contributions to organic nitrogen pools in the ocean. These new results represent the most detailed investigation of CSI-AA data in zooplankton size classes to date, and point to a key role of large zooplankton in the transmission of the diazotrophic nitrogen up oceanic food webs. Malaspina-2010(CSD2008-00077), NSF Grant OCE-1131816