Contribution of Crenarchaea and Bacteria to autotrophy in the North´s Atlantic Interior
Marine Crenarchaeota are among the most abundant groups of prokaryotes in the ocean and recent reports suggest that they oxidize ammonia as an energy source and inorganic carbon as carbon source, while other studies indicate that Crenarchaeota use organic carbon and hence, live heterotrophically. We...
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ftcsic:oai:digital.csic.es:10261/316416 2024-02-11T09:58:31+01:00 Contribution of Crenarchaea and Bacteria to autotrophy in the North´s Atlantic Interior Varela, M.M. (Marta María) Hendrik Sintes, E. (Eva) Reinthaler, T. Van-Aken, H.M. (Hendrik M.) Herndl, G.J. Atlantic Ocean North Atlantic 2014-11-21T10:27:01Z http://hdl.handle.net/10508/8085 http://hdl.handle.net/10261/316416 https://doi.org/10.1111/j.1462-2920.2011.02457.x unknown Centro Oceanográfico de A Coruña 1462-2920 http://hdl.handle.net/10508/8085 http://hdl.handle.net/10261/316416 doi:10.1111/j.1462-2920.2011.02457.x Environmental Microbiology, 13. 2011: 1524-1533 2889 none research article S 2014 ftcsic https://doi.org/10.1111/j.1462-2920.2011.02457.x 2024-01-16T11:44:31Z Marine Crenarchaeota are among the most abundant groups of prokaryotes in the ocean and recent reports suggest that they oxidize ammonia as an energy source and inorganic carbon as carbon source, while other studies indicate that Crenarchaeota use organic carbon and hence, live heterotrophically. We used catalysed reporter deposition fluorescence in situ hybridization (CARD-FISH) to determine the crenarchaeal and bacterial contribution to total prokaryotic abundance in the (sub)tropical Atlantic. Bacteria contributed ∼50% to total prokaryotes throughout the water column. Marine Crenarchaeota Group I (MCGI) accounted for ∼5% of the prokaryotes in subsurface waters (100 m depth) and between 10 and 20% in the oxygen minimum layer (250–500 m depth) and deep waters (North East Atlantic Deep Water). The fraction of both MCGI and Bacteria fixing inorganic carbon, determined by combining microautoradiography with CARD-FISH (MICRO-CARD-FISH), decreased with depth, ranging from ∼30% in the oxygen minimum zone to < 10% in the intermediate waters (Mediterranean Sea Outflow Water, Antarctic Intermediate Water). In the deeper water masses, however, MCGI were not taking up inorganic carbon. Using quantitative MICRO-CARD-FISH to determine autotrophy activity on a single cell level revealed that MCGI are incorporating inorganic carbon (0.002–0.1 fmol C cell−1 day−1) at a significantly lower rate than Bacteria (0.01–0.6 fmol C cell−1 day−1). Hence, it appears that MCGI contribute substantially less to autotrophy than Bacteria. Taking the stoichiometry of nitrification together with our findings suggests that MCGI might not dominate the ammonia oxidation step in the mesopelagic waters of the ocean to that extent as the reported dominance of archaeal over bacterial amoA would suggest. 6 Article in Journal/Newspaper Antarc* Antarctic North Atlantic North East Atlantic Digital.CSIC (Spanish National Research Council) Antarctic Environmental Microbiology 13 6 1524 1533 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
language |
unknown |
description |
Marine Crenarchaeota are among the most abundant groups of prokaryotes in the ocean and recent reports suggest that they oxidize ammonia as an energy source and inorganic carbon as carbon source, while other studies indicate that Crenarchaeota use organic carbon and hence, live heterotrophically. We used catalysed reporter deposition fluorescence in situ hybridization (CARD-FISH) to determine the crenarchaeal and bacterial contribution to total prokaryotic abundance in the (sub)tropical Atlantic. Bacteria contributed ∼50% to total prokaryotes throughout the water column. Marine Crenarchaeota Group I (MCGI) accounted for ∼5% of the prokaryotes in subsurface waters (100 m depth) and between 10 and 20% in the oxygen minimum layer (250–500 m depth) and deep waters (North East Atlantic Deep Water). The fraction of both MCGI and Bacteria fixing inorganic carbon, determined by combining microautoradiography with CARD-FISH (MICRO-CARD-FISH), decreased with depth, ranging from ∼30% in the oxygen minimum zone to < 10% in the intermediate waters (Mediterranean Sea Outflow Water, Antarctic Intermediate Water). In the deeper water masses, however, MCGI were not taking up inorganic carbon. Using quantitative MICRO-CARD-FISH to determine autotrophy activity on a single cell level revealed that MCGI are incorporating inorganic carbon (0.002–0.1 fmol C cell−1 day−1) at a significantly lower rate than Bacteria (0.01–0.6 fmol C cell−1 day−1). Hence, it appears that MCGI contribute substantially less to autotrophy than Bacteria. Taking the stoichiometry of nitrification together with our findings suggests that MCGI might not dominate the ammonia oxidation step in the mesopelagic waters of the ocean to that extent as the reported dominance of archaeal over bacterial amoA would suggest. 6 |
format |
Article in Journal/Newspaper |
author |
Varela, M.M. (Marta María) Hendrik Sintes, E. (Eva) Reinthaler, T. Van-Aken, H.M. (Hendrik M.) Herndl, G.J. |
spellingShingle |
Varela, M.M. (Marta María) Hendrik Sintes, E. (Eva) Reinthaler, T. Van-Aken, H.M. (Hendrik M.) Herndl, G.J. Contribution of Crenarchaea and Bacteria to autotrophy in the North´s Atlantic Interior |
author_facet |
Varela, M.M. (Marta María) Hendrik Sintes, E. (Eva) Reinthaler, T. Van-Aken, H.M. (Hendrik M.) Herndl, G.J. |
author_sort |
Varela, M.M. (Marta María) |
title |
Contribution of Crenarchaea and Bacteria to autotrophy in the North´s Atlantic Interior |
title_short |
Contribution of Crenarchaea and Bacteria to autotrophy in the North´s Atlantic Interior |
title_full |
Contribution of Crenarchaea and Bacteria to autotrophy in the North´s Atlantic Interior |
title_fullStr |
Contribution of Crenarchaea and Bacteria to autotrophy in the North´s Atlantic Interior |
title_full_unstemmed |
Contribution of Crenarchaea and Bacteria to autotrophy in the North´s Atlantic Interior |
title_sort |
contribution of crenarchaea and bacteria to autotrophy in the north´s atlantic interior |
publisher |
Centro Oceanográfico de A Coruña |
publishDate |
2014 |
url |
http://hdl.handle.net/10508/8085 http://hdl.handle.net/10261/316416 https://doi.org/10.1111/j.1462-2920.2011.02457.x |
op_coverage |
Atlantic Ocean North Atlantic |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic North Atlantic North East Atlantic |
genre_facet |
Antarc* Antarctic North Atlantic North East Atlantic |
op_relation |
1462-2920 http://hdl.handle.net/10508/8085 http://hdl.handle.net/10261/316416 doi:10.1111/j.1462-2920.2011.02457.x Environmental Microbiology, 13. 2011: 1524-1533 2889 |
op_rights |
none |
op_doi |
https://doi.org/10.1111/j.1462-2920.2011.02457.x |
container_title |
Environmental Microbiology |
container_volume |
13 |
container_issue |
6 |
container_start_page |
1524 |
op_container_end_page |
1533 |
_version_ |
1790594179410165760 |