Effect of triploidy on turbot haematology
This study was carried out to compare key haematological features of diploid (2n) and triploid (3n) turbot as a first step towards the assessment of the ability of 3n turbot to withstand sub-optimal culture conditions. Morphometric indices of erythrocytes were determined on blood smears by light mic...
Published in: | Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
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Centro Oceanográfico de Vigo
2023
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Online Access: | http://hdl.handle.net/10508/9063 http://hdl.handle.net/10261/315634 https://doi.org/10.1016/j.cbpb.2005.03.007 |
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ftcsic:oai:digital.csic.es:10261/315634 2024-02-11T10:09:16+01:00 Effect of triploidy on turbot haematology Cal, R. (Rosa) Vidal, S. Camacho, T. (Tomás) Piferrer, F. (Francesc) Guitian, F.J. 2023-06-20T18:11:37Z http://hdl.handle.net/10508/9063 http://hdl.handle.net/10261/315634 https://doi.org/10.1016/j.cbpb.2005.03.007 en eng Centro Oceanográfico de Vigo 0300-9629 http://hdl.handle.net/10508/9063 http://hdl.handle.net/10261/315634 doi:10.1016/j.cbpb.2005.03.007 Comparative Biochemistry and Physiology A, 141. 2005: 35-41 20804 open Turbot Triploidy Blood Erythrocyte Haematology Haemoglobin Oxygen transport research article VoR SI 2023 ftcsic https://doi.org/10.1016/j.cbpb.2005.03.007 2024-01-16T11:44:22Z This study was carried out to compare key haematological features of diploid (2n) and triploid (3n) turbot as a first step towards the assessment of the ability of 3n turbot to withstand sub-optimal culture conditions. Morphometric indices of erythrocytes were determined on blood smears by light microscopy. Triploidy significantly (P < 0.001) increased all morphometric indices measured in the erythrocytes, including size, surface, and volume, except for the size of minor nuclear axis. The increase in cell size was larger for the major (31.0%) than for the minor (8.3%) axis, thus rendering erythrocytes of 3n turbot more ellipsoidal. The increase in erythrocyte volume (45.9%) was close to the theoretical expected 50% increase as a result of one extra chromosome set. Haematological indices were measured automatically by a haematological Coulter Counter. Triploid turbot had lower numbers of red blood cells (RBC: 1.84 cells pL 1 in 2n vs. 1.27 cells pL 1 in 3n; P < 0.001) but they were of a larger size (Mean corpuscular volume [MCV]: 145.51 fL in 2n vs. 181.78 fL in 3n; P < 0.001). However, the decrease in RBC was not compensated by the increase in MCV, and thus, triploidy decreased the haematocrit (Hct: 26.80% in 2n vs. 23.11% in 3n; P < 0.001) and total blood haemoglobin concentration (Hb: 73.74 g l 1 in 2n vs. 67.54 g l 1 in 3n; P < 0.05). In contrast, mean corpuscular hemoglobin (MCH: 40.27 pg in 2n vs. 53.28 pg in 3n; P < 0.001) was higher for 3n turbot as a result of their larger erythrocytes although MCH concentration (MCHC: 0.28 pg fL 1 in 2n vs. 0.29 pg fL 1 in 3n did not significantly differ. RBC, Hct and MCV were also determined manually using light microscopy. In general, discrepancies between the two methods were small (overall ¨7%) but the Coulter Counter tended to overestimate RBC and Hct (and thus to underestimate MCV). Nevertheless, relative differences between ploidies were very similar, thus verifying triploidy-associated changes in hematological features. These changes, as ... Article in Journal/Newspaper Turbot Digital.CSIC (Spanish National Research Council) Coulter ENVELOPE(-58.033,-58.033,-83.283,-83.283) Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 141 1 35 41 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Turbot Triploidy Blood Erythrocyte Haematology Haemoglobin Oxygen transport |
spellingShingle |
Turbot Triploidy Blood Erythrocyte Haematology Haemoglobin Oxygen transport Cal, R. (Rosa) Vidal, S. Camacho, T. (Tomás) Piferrer, F. (Francesc) Guitian, F.J. Effect of triploidy on turbot haematology |
topic_facet |
Turbot Triploidy Blood Erythrocyte Haematology Haemoglobin Oxygen transport |
description |
This study was carried out to compare key haematological features of diploid (2n) and triploid (3n) turbot as a first step towards the assessment of the ability of 3n turbot to withstand sub-optimal culture conditions. Morphometric indices of erythrocytes were determined on blood smears by light microscopy. Triploidy significantly (P < 0.001) increased all morphometric indices measured in the erythrocytes, including size, surface, and volume, except for the size of minor nuclear axis. The increase in cell size was larger for the major (31.0%) than for the minor (8.3%) axis, thus rendering erythrocytes of 3n turbot more ellipsoidal. The increase in erythrocyte volume (45.9%) was close to the theoretical expected 50% increase as a result of one extra chromosome set. Haematological indices were measured automatically by a haematological Coulter Counter. Triploid turbot had lower numbers of red blood cells (RBC: 1.84 cells pL 1 in 2n vs. 1.27 cells pL 1 in 3n; P < 0.001) but they were of a larger size (Mean corpuscular volume [MCV]: 145.51 fL in 2n vs. 181.78 fL in 3n; P < 0.001). However, the decrease in RBC was not compensated by the increase in MCV, and thus, triploidy decreased the haematocrit (Hct: 26.80% in 2n vs. 23.11% in 3n; P < 0.001) and total blood haemoglobin concentration (Hb: 73.74 g l 1 in 2n vs. 67.54 g l 1 in 3n; P < 0.05). In contrast, mean corpuscular hemoglobin (MCH: 40.27 pg in 2n vs. 53.28 pg in 3n; P < 0.001) was higher for 3n turbot as a result of their larger erythrocytes although MCH concentration (MCHC: 0.28 pg fL 1 in 2n vs. 0.29 pg fL 1 in 3n did not significantly differ. RBC, Hct and MCV were also determined manually using light microscopy. In general, discrepancies between the two methods were small (overall ¨7%) but the Coulter Counter tended to overestimate RBC and Hct (and thus to underestimate MCV). Nevertheless, relative differences between ploidies were very similar, thus verifying triploidy-associated changes in hematological features. These changes, as ... |
format |
Article in Journal/Newspaper |
author |
Cal, R. (Rosa) Vidal, S. Camacho, T. (Tomás) Piferrer, F. (Francesc) Guitian, F.J. |
author_facet |
Cal, R. (Rosa) Vidal, S. Camacho, T. (Tomás) Piferrer, F. (Francesc) Guitian, F.J. |
author_sort |
Cal, R. (Rosa) |
title |
Effect of triploidy on turbot haematology |
title_short |
Effect of triploidy on turbot haematology |
title_full |
Effect of triploidy on turbot haematology |
title_fullStr |
Effect of triploidy on turbot haematology |
title_full_unstemmed |
Effect of triploidy on turbot haematology |
title_sort |
effect of triploidy on turbot haematology |
publisher |
Centro Oceanográfico de Vigo |
publishDate |
2023 |
url |
http://hdl.handle.net/10508/9063 http://hdl.handle.net/10261/315634 https://doi.org/10.1016/j.cbpb.2005.03.007 |
long_lat |
ENVELOPE(-58.033,-58.033,-83.283,-83.283) |
geographic |
Coulter |
geographic_facet |
Coulter |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
0300-9629 http://hdl.handle.net/10508/9063 http://hdl.handle.net/10261/315634 doi:10.1016/j.cbpb.2005.03.007 Comparative Biochemistry and Physiology A, 141. 2005: 35-41 20804 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.cbpb.2005.03.007 |
container_title |
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology |
container_volume |
141 |
container_issue |
1 |
container_start_page |
35 |
op_container_end_page |
41 |
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1790609069001670656 |