Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis
SPARC/osteonectin is amultifunctionalmatricellular glycoprotein,which is expressed in embryonic and adult tissues that undergo active proliferation and dynamic morphogenesis. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during devel...
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Centro Oceanográfico de Vigo
2023
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Online Access: | http://hdl.handle.net/10508/9079 http://hdl.handle.net/10261/315588 https://doi.org/10.1016/j.gene.2015.05.036 |
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ftcsic:oai:digital.csic.es:10261/315588 2024-02-11T10:08:27+01:00 Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis Torres-Núñez, E. Suárez-Bregua, P. Cal, L. (Laura) Cal, R. (Rosa) Cerdá-Reverter, J.M. (José Miguel) Rotllant, J. (Josep) 2023-06-20T18:10:40Z http://hdl.handle.net/10508/9079 http://hdl.handle.net/10261/315588 https://doi.org/10.1016/j.gene.2015.05.036 en eng Centro Oceanográfico de Vigo http://www.sciencedirect.com/science/article/pii/S0378111915006083 http://hdl.handle.net/10508/9079 http://hdl.handle.net/10261/315588 doi:10.1016/j.gene.2015.05.036 Gene, 568 (2). 2015: 129-139 20818 open Osteonectin Sparc Turbot Gene expression Metamorphosis Post-embryonic development research article AM SI 2023 ftcsic https://doi.org/10.1016/j.gene.2015.05.036 2024-01-16T11:44:20Z SPARC/osteonectin is amultifunctionalmatricellular glycoprotein,which is expressed in embryonic and adult tissues that undergo active proliferation and dynamic morphogenesis. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during development are largely unknown. In this report, we describe the isolation, characterization, post-embryonic developmental expression and environmental thermal regulation of sparc in turbot. The full-length turbot sparc cDNA contains 930 bp and encodes a protein of 310 amino acids, which shares 77, 75 and 80% identity with human, frog and zebrafish, respectively. Results of whole-mount in situ hybridization reveal a dynamic expression profile during post-embryonic turbot development. Sparc is expressed differentially in the cranioencephalic region; mainly in jaws, branchial arches, fin folds and rays of caudal, dorsal and anal fins. Furthermore, ontogenetic studies demonstrated that Sparc gene expression is dynamically regulated during post-embryonic turbot development, with high expression during stage-specific post-embryonic remodeling. Additionally, the effect of thermal environmental conditions on turbot development and on ontogenetic sparc expression was evaluated. Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Gene 568 2 129 139 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Osteonectin Sparc Turbot Gene expression Metamorphosis Post-embryonic development |
spellingShingle |
Osteonectin Sparc Turbot Gene expression Metamorphosis Post-embryonic development Torres-Núñez, E. Suárez-Bregua, P. Cal, L. (Laura) Cal, R. (Rosa) Cerdá-Reverter, J.M. (José Miguel) Rotllant, J. (Josep) Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
topic_facet |
Osteonectin Sparc Turbot Gene expression Metamorphosis Post-embryonic development |
description |
SPARC/osteonectin is amultifunctionalmatricellular glycoprotein,which is expressed in embryonic and adult tissues that undergo active proliferation and dynamic morphogenesis. Recent studies indicate that Sparc expression appears early in development, although its function and regulation during development are largely unknown. In this report, we describe the isolation, characterization, post-embryonic developmental expression and environmental thermal regulation of sparc in turbot. The full-length turbot sparc cDNA contains 930 bp and encodes a protein of 310 amino acids, which shares 77, 75 and 80% identity with human, frog and zebrafish, respectively. Results of whole-mount in situ hybridization reveal a dynamic expression profile during post-embryonic turbot development. Sparc is expressed differentially in the cranioencephalic region; mainly in jaws, branchial arches, fin folds and rays of caudal, dorsal and anal fins. Furthermore, ontogenetic studies demonstrated that Sparc gene expression is dynamically regulated during post-embryonic turbot development, with high expression during stage-specific post-embryonic remodeling. Additionally, the effect of thermal environmental conditions on turbot development and on ontogenetic sparc expression was evaluated. |
format |
Article in Journal/Newspaper |
author |
Torres-Núñez, E. Suárez-Bregua, P. Cal, L. (Laura) Cal, R. (Rosa) Cerdá-Reverter, J.M. (José Miguel) Rotllant, J. (Josep) |
author_facet |
Torres-Núñez, E. Suárez-Bregua, P. Cal, L. (Laura) Cal, R. (Rosa) Cerdá-Reverter, J.M. (José Miguel) Rotllant, J. (Josep) |
author_sort |
Torres-Núñez, E. |
title |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_short |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_full |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_fullStr |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_full_unstemmed |
Molecular cloning and characterization of the matricellular protein Sparc/osteonectin in flatfish, Scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
title_sort |
molecular cloning and characterization of the matricellular protein sparc/osteonectin in flatfish, scophthalmus maximus, and its developmental stage-dependent transcriptional regulation during metamorphosis |
publisher |
Centro Oceanográfico de Vigo |
publishDate |
2023 |
url |
http://hdl.handle.net/10508/9079 http://hdl.handle.net/10261/315588 https://doi.org/10.1016/j.gene.2015.05.036 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://www.sciencedirect.com/science/article/pii/S0378111915006083 http://hdl.handle.net/10508/9079 http://hdl.handle.net/10261/315588 doi:10.1016/j.gene.2015.05.036 Gene, 568 (2). 2015: 129-139 20818 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.gene.2015.05.036 |
container_title |
Gene |
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568 |
container_issue |
2 |
container_start_page |
129 |
op_container_end_page |
139 |
_version_ |
1790607786915135488 |