Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.)
An enzymoimmunoassay (EIA) kit for plasma melatonin (MLT) measurements was employed in tench (Tinca tinca) and in turbot (Scophtalmus maximus). Tench and turbot plasma samples were purified with a C18 reversed phase extraction columns because this kit is designed for human serum measurements. The lo...
Main Authors: | , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Centro Oceanográfico de Vigo
2014
|
Subjects: | |
Online Access: | http://hdl.handle.net/10508/6915 http://hdl.handle.net/10261/315410 |
id |
ftcsic:oai:digital.csic.es:10261/315410 |
---|---|
record_format |
openpolar |
spelling |
ftcsic:oai:digital.csic.es:10261/315410 2024-02-11T10:08:27+01:00 Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) Rebollar, P.J. Ubilla, E. Peleteiro-Alonso, J.B. (José Benito) Alvariño, M. 2014-11-21T09:56:31Z http://hdl.handle.net/10508/6915 http://hdl.handle.net/10261/315410 unknown Centro Oceanográfico de Vigo http://hdl.handle.net/10508/6915 http://hdl.handle.net/10261/315410 Journal of Physiology and Biochemistry, 55(4). 1999: 341-348 1213 open research article S 2014 ftcsic 2024-01-16T11:44:14Z An enzymoimmunoassay (EIA) kit for plasma melatonin (MLT) measurements was employed in tench (Tinca tinca) and in turbot (Scophtalmus maximus). Tench and turbot plasma samples were purified with a C18 reversed phase extraction columns because this kit is designed for human serum measurements. The lowest detection limit of the technique was 11.48 pg/well with a sensitivity at 50% binding of 100 pg/well. Intra-assay and inter-assay CV (%) were always less than 5% (n=8), and 9% (n=6) in tench plasma samples, and less than 5% (n=8) and 13% (n=5) in turbot plasma samples, respectively. Correlation coefficients between EIA and RIA measurements in tench and turbot plasma samples were 0.93 and 0.89 (p<0.001) respectively. Diurnal and nocturnal plasma melatonin mean levels were 14.7+/-2.1 pg/ml and 87.4+/-11 pg/ml in tench (n=15), and 3.5+/-0.4 pg/ml and 28.1+/-2.1 pg/ml in turbot (n=15). These species showed a melatonin circadian rhythm as in other animals studied. The results suggest that the commercial kit used in this experiment could be a suitable and alternative method to RIA for plasma MLT determinations in tench and turbot although it is necessary to increase volumes (1ml) and concentrate daytime samples. 1 Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Eia ENVELOPE(7.755,7.755,63.024,63.024) |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
description |
An enzymoimmunoassay (EIA) kit for plasma melatonin (MLT) measurements was employed in tench (Tinca tinca) and in turbot (Scophtalmus maximus). Tench and turbot plasma samples were purified with a C18 reversed phase extraction columns because this kit is designed for human serum measurements. The lowest detection limit of the technique was 11.48 pg/well with a sensitivity at 50% binding of 100 pg/well. Intra-assay and inter-assay CV (%) were always less than 5% (n=8), and 9% (n=6) in tench plasma samples, and less than 5% (n=8) and 13% (n=5) in turbot plasma samples, respectively. Correlation coefficients between EIA and RIA measurements in tench and turbot plasma samples were 0.93 and 0.89 (p<0.001) respectively. Diurnal and nocturnal plasma melatonin mean levels were 14.7+/-2.1 pg/ml and 87.4+/-11 pg/ml in tench (n=15), and 3.5+/-0.4 pg/ml and 28.1+/-2.1 pg/ml in turbot (n=15). These species showed a melatonin circadian rhythm as in other animals studied. The results suggest that the commercial kit used in this experiment could be a suitable and alternative method to RIA for plasma MLT determinations in tench and turbot although it is necessary to increase volumes (1ml) and concentrate daytime samples. 1 |
format |
Article in Journal/Newspaper |
author |
Rebollar, P.J. Ubilla, E. Peleteiro-Alonso, J.B. (José Benito) Alvariño, M. |
spellingShingle |
Rebollar, P.J. Ubilla, E. Peleteiro-Alonso, J.B. (José Benito) Alvariño, M. Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
author_facet |
Rebollar, P.J. Ubilla, E. Peleteiro-Alonso, J.B. (José Benito) Alvariño, M. |
author_sort |
Rebollar, P.J. |
title |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_short |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_full |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_fullStr |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_full_unstemmed |
Determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (EIA) in turbot (Scophthalmus maximus L.) and tench (Tinca tinca L.) |
title_sort |
determination of plasma melatonin levels by enzyme-linked inmunoabsobent assay (eia) in turbot (scophthalmus maximus l.) and tench (tinca tinca l.) |
publisher |
Centro Oceanográfico de Vigo |
publishDate |
2014 |
url |
http://hdl.handle.net/10508/6915 http://hdl.handle.net/10261/315410 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://hdl.handle.net/10508/6915 http://hdl.handle.net/10261/315410 Journal of Physiology and Biochemistry, 55(4). 1999: 341-348 1213 |
op_rights |
open |
_version_ |
1790607788262555648 |