DMSO influx in turbot embryos exposed to a vitrification protocol
The particular characteristics of fish embryos require the development of specific methods for cryopreservation. One of the main obstacles is related to the presence of membranes and compartments with different water and cryoprotectant permeability. To assess dimethyl sulfoxide (Me2SO4) permeability...
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2003
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Online Access: | http://hdl.handle.net/10508/7200 http://hdl.handle.net/10261/314121 https://doi.org/10.1016/S0093-691X(03)00033-5 |
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ftcsic:oai:digital.csic.es:10261/314121 2024-02-11T10:08:27+01:00 DMSO influx in turbot embryos exposed to a vitrification protocol Cabrita, Elsa Robles, Vanesa Chereguini-Fernández-Maquieira María del Olvido Paz, P. Anel, L. Herráez, Paz 2003 http://hdl.handle.net/10508/7200 http://hdl.handle.net/10261/314121 https://doi.org/10.1016/S0093-691X(03)00033-5 unknown Centro Oceanográfico de Santander Theriogenology, 60. 2003: 463-473 0093-691X http://hdl.handle.net/10508/7200 http://hdl.handle.net/10261/314121 doi:10.1016/S0093-691X(03)00033-5 5254 none Centro Oceanográfico de Santander Acuicultura research article 2003 ftcsic https://doi.org/10.1016/S0093-691X(03)00033-5 2024-01-16T11:44:04Z The particular characteristics of fish embryos require the development of specific methods for cryopreservation. One of the main obstacles is related to the presence of membranes and compartments with different water and cryoprotectant permeability. To assess dimethyl sulfoxide (Me2SO4) permeability, we exposed turbot embryos (Scophthalmus maximus) at F stage (tail bud) to the cryoprotectant solutions used in a vitrification protocol and then evaluated the Me2SO4 content inside the embryo using high-performance liquid chromatography (HPLC). The Me2SO4 influx was analyzed in normal embryos and in embryos treated with pronase (2 mg/ml) in order to increase chorion permeability. The evaluation was made after each step of cryoprotectant incorporation and removal. Three embryo compartments were distinguished: the perivitelline space (PVS), the yolk sac (YS) and the cellular compartment (CC), and the relative volumes of each, estimated using stereoscopic microscopy imaging, were 11.37, 81.23 and 7.40%, respectively. The Me2SO4 concentration inside the embryos was calculated based on their entrance into one, two or three compartments. Results suggest high entrance of Me2SO4 into the PVS and a low concentration of this cryoprotectant inside the other compartments. Pronase did not significantly increase Me2SO4 influx, but facilitated its elimination during the washing steps. Sí Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Theriogenology 60 3 463 473 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Centro Oceanográfico de Santander Acuicultura |
spellingShingle |
Centro Oceanográfico de Santander Acuicultura Cabrita, Elsa Robles, Vanesa Chereguini-Fernández-Maquieira María del Olvido Paz, P. Anel, L. Herráez, Paz DMSO influx in turbot embryos exposed to a vitrification protocol |
topic_facet |
Centro Oceanográfico de Santander Acuicultura |
description |
The particular characteristics of fish embryos require the development of specific methods for cryopreservation. One of the main obstacles is related to the presence of membranes and compartments with different water and cryoprotectant permeability. To assess dimethyl sulfoxide (Me2SO4) permeability, we exposed turbot embryos (Scophthalmus maximus) at F stage (tail bud) to the cryoprotectant solutions used in a vitrification protocol and then evaluated the Me2SO4 content inside the embryo using high-performance liquid chromatography (HPLC). The Me2SO4 influx was analyzed in normal embryos and in embryos treated with pronase (2 mg/ml) in order to increase chorion permeability. The evaluation was made after each step of cryoprotectant incorporation and removal. Three embryo compartments were distinguished: the perivitelline space (PVS), the yolk sac (YS) and the cellular compartment (CC), and the relative volumes of each, estimated using stereoscopic microscopy imaging, were 11.37, 81.23 and 7.40%, respectively. The Me2SO4 concentration inside the embryos was calculated based on their entrance into one, two or three compartments. Results suggest high entrance of Me2SO4 into the PVS and a low concentration of this cryoprotectant inside the other compartments. Pronase did not significantly increase Me2SO4 influx, but facilitated its elimination during the washing steps. Sí |
format |
Article in Journal/Newspaper |
author |
Cabrita, Elsa Robles, Vanesa Chereguini-Fernández-Maquieira María del Olvido Paz, P. Anel, L. Herráez, Paz |
author_facet |
Cabrita, Elsa Robles, Vanesa Chereguini-Fernández-Maquieira María del Olvido Paz, P. Anel, L. Herráez, Paz |
author_sort |
Cabrita, Elsa |
title |
DMSO influx in turbot embryos exposed to a vitrification protocol |
title_short |
DMSO influx in turbot embryos exposed to a vitrification protocol |
title_full |
DMSO influx in turbot embryos exposed to a vitrification protocol |
title_fullStr |
DMSO influx in turbot embryos exposed to a vitrification protocol |
title_full_unstemmed |
DMSO influx in turbot embryos exposed to a vitrification protocol |
title_sort |
dmso influx in turbot embryos exposed to a vitrification protocol |
publishDate |
2003 |
url |
http://hdl.handle.net/10508/7200 http://hdl.handle.net/10261/314121 https://doi.org/10.1016/S0093-691X(03)00033-5 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
Centro Oceanográfico de Santander Theriogenology, 60. 2003: 463-473 0093-691X http://hdl.handle.net/10508/7200 http://hdl.handle.net/10261/314121 doi:10.1016/S0093-691X(03)00033-5 5254 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/S0093-691X(03)00033-5 |
container_title |
Theriogenology |
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60 |
container_issue |
3 |
container_start_page |
463 |
op_container_end_page |
473 |
_version_ |
1790607782784794624 |