A sandwich ELISA to detect VHSV and IPNV in turbot
Abstract: The recent demonstration that reared turbot (Scophthalmus maximus L) is a natural host for salmonid rhabdoviruses has made their rapid detection relevant to these fish species. A unique protocol to select and use non-competitive monoclonal antibodies (Mabs) for two high-sensitivity sandwic...
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Language: | English |
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Kluwer Academic Publishers
1994
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Online Access: | http://hdl.handle.net/10508/1765 http://hdl.handle.net/10261/312668 https://doi.org/10.1007/BF00231516 |
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ftcsic:oai:digital.csic.es:10261/312668 2024-02-11T10:08:26+01:00 A sandwich ELISA to detect VHSV and IPNV in turbot Vázquez-Brañas, Manuel Coll-Morales, Julio Estepa, Amparo Atlantic Ocean Central Atlantic Eastern Central Atlantic Northwest Spanish 1991 1994-09-01 http://hdl.handle.net/10508/1765 http://hdl.handle.net/10261/312668 https://doi.org/10.1007/BF00231516 en eng Kluwer Academic Publishers Centro Oceanográfico de A Coruña Aquaculture International, vol. 2, issue 3, pp: 206-217. Sept., 1994. 0967-6120 Online ISSN: 1573-143X http://hdl.handle.net/10508/1765 http://hdl.handle.net/10261/312668 doi:10.1007/BF00231516 open Centro Oceanográfico de A Coruña Acuicultura Turbot Scophthalmus maximus Viral diseases VHSV IPNV Mabs Diagnosis ELISA research article 1994 ftcsic https://doi.org/10.1007/BF00231516 2024-01-16T11:43:25Z Abstract: The recent demonstration that reared turbot (Scophthalmus maximus L) is a natural host for salmonid rhabdoviruses has made their rapid detection relevant to these fish species. A unique protocol to select and use non-competitive monoclonal antibodies (Mabs) for two high-sensitivity sandwich ELISAs has been developed to detect both infectious pancreatic necrosis virus (IPNV) and viral haemorrhagic septicaemia virus (VHSV) in turbot kidney extracts to assess the possibility of using them in field diagnosis. For maximum sensitivity, turbot kidney extracts can be two-fold diluted with high-ionic strength buffers and assayed for the presence of the major viral proteins (VMS rhabdovirus nucleoprotein N/Nx and/or IPN birnavirus protein VP3). The use of control plates coated with irrelevant mouse antibodies (IgG1 and IgG2a) in parallel ELISAs allows for a precise estimation of possible false positives. Turbot kidney extracts with low levels of virus might now be assayed directly without using cell culture, with high precision and in a short time during the acute phase of these viral diseases in reared turbot. Acuidoro, SL; INIA peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Aquaculture International 2 3 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Centro Oceanográfico de A Coruña Acuicultura Turbot Scophthalmus maximus Viral diseases VHSV IPNV Mabs Diagnosis ELISA |
spellingShingle |
Centro Oceanográfico de A Coruña Acuicultura Turbot Scophthalmus maximus Viral diseases VHSV IPNV Mabs Diagnosis ELISA Vázquez-Brañas, Manuel Coll-Morales, Julio Estepa, Amparo A sandwich ELISA to detect VHSV and IPNV in turbot |
topic_facet |
Centro Oceanográfico de A Coruña Acuicultura Turbot Scophthalmus maximus Viral diseases VHSV IPNV Mabs Diagnosis ELISA |
description |
Abstract: The recent demonstration that reared turbot (Scophthalmus maximus L) is a natural host for salmonid rhabdoviruses has made their rapid detection relevant to these fish species. A unique protocol to select and use non-competitive monoclonal antibodies (Mabs) for two high-sensitivity sandwich ELISAs has been developed to detect both infectious pancreatic necrosis virus (IPNV) and viral haemorrhagic septicaemia virus (VHSV) in turbot kidney extracts to assess the possibility of using them in field diagnosis. For maximum sensitivity, turbot kidney extracts can be two-fold diluted with high-ionic strength buffers and assayed for the presence of the major viral proteins (VMS rhabdovirus nucleoprotein N/Nx and/or IPN birnavirus protein VP3). The use of control plates coated with irrelevant mouse antibodies (IgG1 and IgG2a) in parallel ELISAs allows for a precise estimation of possible false positives. Turbot kidney extracts with low levels of virus might now be assayed directly without using cell culture, with high precision and in a short time during the acute phase of these viral diseases in reared turbot. Acuidoro, SL; INIA peer reviewed |
format |
Article in Journal/Newspaper |
author |
Vázquez-Brañas, Manuel Coll-Morales, Julio Estepa, Amparo |
author_facet |
Vázquez-Brañas, Manuel Coll-Morales, Julio Estepa, Amparo |
author_sort |
Vázquez-Brañas, Manuel |
title |
A sandwich ELISA to detect VHSV and IPNV in turbot |
title_short |
A sandwich ELISA to detect VHSV and IPNV in turbot |
title_full |
A sandwich ELISA to detect VHSV and IPNV in turbot |
title_fullStr |
A sandwich ELISA to detect VHSV and IPNV in turbot |
title_full_unstemmed |
A sandwich ELISA to detect VHSV and IPNV in turbot |
title_sort |
sandwich elisa to detect vhsv and ipnv in turbot |
publisher |
Kluwer Academic Publishers |
publishDate |
1994 |
url |
http://hdl.handle.net/10508/1765 http://hdl.handle.net/10261/312668 https://doi.org/10.1007/BF00231516 |
op_coverage |
Atlantic Ocean Central Atlantic Eastern Central Atlantic Northwest Spanish 1991 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
Centro Oceanográfico de A Coruña Aquaculture International, vol. 2, issue 3, pp: 206-217. Sept., 1994. 0967-6120 Online ISSN: 1573-143X http://hdl.handle.net/10508/1765 http://hdl.handle.net/10261/312668 doi:10.1007/BF00231516 |
op_rights |
open |
op_doi |
https://doi.org/10.1007/BF00231516 |
container_title |
Aquaculture International |
container_volume |
2 |
container_issue |
3 |
_version_ |
1790607753688907776 |