DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus
17 Pág. Ectomycorrhizas are an intrinsic component of tree nutrition and responses to environmental variations. How epigenetic mechanisms might regulate these mutualistic interactions is unknown. By manipulating the level of expression of the chromatin remodeler DECREASE IN DNA METHYLATION 1 (DDM1)...
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ftcsic:oai:digital.csic.es:10261/311773 2024-02-11T10:03:22+01:00 DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus Vigneaud, Julien Kohler, Annegret Sow, Mamadou Dia Delaunay, Alain Fauchery, Laure Guinet, Frederic Daviaud, Christian Barry, Kerrie W. Keymanesh, Keykhosrow Johnson, Jenifer Singan, Vasanth Grigoriev, Igor Fichot, Régis Conde, Daniel Perales, Mariano Tost, Jörg Martin, Francis M. Allona, Isabel Strauss, Steven H. Veneault-Fourrey, Claire Maury, Stéphane Conseil Régional Région Centre Val de Loire Department of Energy (US) Kohler, Annegret Sow, Mamadou Dia Delaunay, Alain Fauchery, Laure Barry, Kerrie W. Keymanesh, Keykhosrow Singan, Vasanth Grigoriev, Igor Fichot, Régis Conde, Daniel Perales, Mariano Tost, Jörg Martin, Francis M. Allona, Isabel Strauss, Steven H. Veneault-Fourrey, Claire Maury, Stéphane 2023-02-20 application/pdf http://hdl.handle.net/10261/311773 https://doi.org/10.1111/nph.18734 https://api.elsevier.com/content/abstract/scopus_id/85148497236 unknown New Phytologist Foundation Centro de Biotecnología y Genómica de Plantas (CBGP) https://doi.org/10.1111/nph.18734 Sí New Phytologist 238(6): 2561-2577 (2023) 0028-646X http://hdl.handle.net/10261/311773 doi:10.1111/nph.18734 1469-8137 36807327 2-s2.0-85148497236 https://api.elsevier.com/content/abstract/scopus_id/85148497236 open DEMETER DNA METHYLATION 1 DNA demethylatione Laccaria bicolor Mycorrhizas Pigenetic Poplar artículo 2023 ftcsic https://doi.org/10.1111/nph.18734 2024-01-16T11:43:13Z 17 Pág. Ectomycorrhizas are an intrinsic component of tree nutrition and responses to environmental variations. How epigenetic mechanisms might regulate these mutualistic interactions is unknown. By manipulating the level of expression of the chromatin remodeler DECREASE IN DNA METHYLATION 1 (DDM1) and two demethylases DEMETER-LIKE (DML) in Populus tremula × Populus alba lines, we examined how host DNA methylation modulates multiple parameters of the responses to root colonization with the mutualistic fungus Laccaria bicolor. We compared the ectomycorrhizas formed between transgenic and wild-type (WT) trees and analyzed their methylomes and transcriptomes. The poplar lines displaying lower mycorrhiza formation rate corresponded to hypomethylated overexpressing DML or RNAi-ddm1 lines. We found 86 genes and 288 transposable elements (TEs) differentially methylated between WT and hypomethylated lines (common to both OX-dml and RNAi-ddm1) and 120 genes/1441 TEs in the fungal genome suggesting a host-induced remodeling of the fungal methylome. Hypomethylated poplar lines displayed 205 differentially expressed genes (cis and trans effects) in common with 17 being differentially methylated (cis). Our findings suggest a central role of host and fungal DNA methylation in the ability to form ectomycorrhizas including not only poplar genes involved in root initiation, ethylene and jasmonate-mediated pathways, and immune response but also terpenoid metabolism. MDS and JV received PhD grants from the MRES and Conseil Régional Région Centre Val de Loire, respectively. The LBLGC and the LEE/CNRGH benefit from the support of the ANR EPITREE (ANR-17-CE32-0009-01, https://www6.inrae.fr/epitree-project_eng/) to SM and JT. We thank RTP3E CNRS and IHPE for the access to the bioinformatic platform (http://galaxy.univ-perp.fr/, Christian Chaparro, France). CVF, AK, FM, FG, and LF benefit from the support of the Genomic Science Program (project ‘Plant-Microbe Interface’), US Department of Energy, Office of Science, Biological and ... Article in Journal/Newspaper DML Digital.CSIC (Spanish National Research Council) Conseil ENVELOPE(-67.433,-67.433,-67.596,-67.596) New Phytologist 238 6 2561 2577 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
DEMETER DNA METHYLATION 1 DNA demethylatione Laccaria bicolor Mycorrhizas Pigenetic Poplar |
spellingShingle |
DEMETER DNA METHYLATION 1 DNA demethylatione Laccaria bicolor Mycorrhizas Pigenetic Poplar Vigneaud, Julien Kohler, Annegret Sow, Mamadou Dia Delaunay, Alain Fauchery, Laure Guinet, Frederic Daviaud, Christian Barry, Kerrie W. Keymanesh, Keykhosrow Johnson, Jenifer Singan, Vasanth Grigoriev, Igor Fichot, Régis Conde, Daniel Perales, Mariano Tost, Jörg Martin, Francis M. Allona, Isabel Strauss, Steven H. Veneault-Fourrey, Claire Maury, Stéphane DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus |
topic_facet |
DEMETER DNA METHYLATION 1 DNA demethylatione Laccaria bicolor Mycorrhizas Pigenetic Poplar |
description |
17 Pág. Ectomycorrhizas are an intrinsic component of tree nutrition and responses to environmental variations. How epigenetic mechanisms might regulate these mutualistic interactions is unknown. By manipulating the level of expression of the chromatin remodeler DECREASE IN DNA METHYLATION 1 (DDM1) and two demethylases DEMETER-LIKE (DML) in Populus tremula × Populus alba lines, we examined how host DNA methylation modulates multiple parameters of the responses to root colonization with the mutualistic fungus Laccaria bicolor. We compared the ectomycorrhizas formed between transgenic and wild-type (WT) trees and analyzed their methylomes and transcriptomes. The poplar lines displaying lower mycorrhiza formation rate corresponded to hypomethylated overexpressing DML or RNAi-ddm1 lines. We found 86 genes and 288 transposable elements (TEs) differentially methylated between WT and hypomethylated lines (common to both OX-dml and RNAi-ddm1) and 120 genes/1441 TEs in the fungal genome suggesting a host-induced remodeling of the fungal methylome. Hypomethylated poplar lines displayed 205 differentially expressed genes (cis and trans effects) in common with 17 being differentially methylated (cis). Our findings suggest a central role of host and fungal DNA methylation in the ability to form ectomycorrhizas including not only poplar genes involved in root initiation, ethylene and jasmonate-mediated pathways, and immune response but also terpenoid metabolism. MDS and JV received PhD grants from the MRES and Conseil Régional Région Centre Val de Loire, respectively. The LBLGC and the LEE/CNRGH benefit from the support of the ANR EPITREE (ANR-17-CE32-0009-01, https://www6.inrae.fr/epitree-project_eng/) to SM and JT. We thank RTP3E CNRS and IHPE for the access to the bioinformatic platform (http://galaxy.univ-perp.fr/, Christian Chaparro, France). CVF, AK, FM, FG, and LF benefit from the support of the Genomic Science Program (project ‘Plant-Microbe Interface’), US Department of Energy, Office of Science, Biological and ... |
author2 |
Conseil Régional Région Centre Val de Loire Department of Energy (US) Kohler, Annegret Sow, Mamadou Dia Delaunay, Alain Fauchery, Laure Barry, Kerrie W. Keymanesh, Keykhosrow Singan, Vasanth Grigoriev, Igor Fichot, Régis Conde, Daniel Perales, Mariano Tost, Jörg Martin, Francis M. Allona, Isabel Strauss, Steven H. Veneault-Fourrey, Claire Maury, Stéphane |
format |
Article in Journal/Newspaper |
author |
Vigneaud, Julien Kohler, Annegret Sow, Mamadou Dia Delaunay, Alain Fauchery, Laure Guinet, Frederic Daviaud, Christian Barry, Kerrie W. Keymanesh, Keykhosrow Johnson, Jenifer Singan, Vasanth Grigoriev, Igor Fichot, Régis Conde, Daniel Perales, Mariano Tost, Jörg Martin, Francis M. Allona, Isabel Strauss, Steven H. Veneault-Fourrey, Claire Maury, Stéphane |
author_facet |
Vigneaud, Julien Kohler, Annegret Sow, Mamadou Dia Delaunay, Alain Fauchery, Laure Guinet, Frederic Daviaud, Christian Barry, Kerrie W. Keymanesh, Keykhosrow Johnson, Jenifer Singan, Vasanth Grigoriev, Igor Fichot, Régis Conde, Daniel Perales, Mariano Tost, Jörg Martin, Francis M. Allona, Isabel Strauss, Steven H. Veneault-Fourrey, Claire Maury, Stéphane |
author_sort |
Vigneaud, Julien |
title |
DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus |
title_short |
DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus |
title_full |
DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus |
title_fullStr |
DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus |
title_full_unstemmed |
DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus |
title_sort |
dna hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus |
publisher |
New Phytologist Foundation |
publishDate |
2023 |
url |
http://hdl.handle.net/10261/311773 https://doi.org/10.1111/nph.18734 https://api.elsevier.com/content/abstract/scopus_id/85148497236 |
long_lat |
ENVELOPE(-67.433,-67.433,-67.596,-67.596) |
geographic |
Conseil |
geographic_facet |
Conseil |
genre |
DML |
genre_facet |
DML |
op_relation |
Centro de Biotecnología y Genómica de Plantas (CBGP) https://doi.org/10.1111/nph.18734 Sí New Phytologist 238(6): 2561-2577 (2023) 0028-646X http://hdl.handle.net/10261/311773 doi:10.1111/nph.18734 1469-8137 36807327 2-s2.0-85148497236 https://api.elsevier.com/content/abstract/scopus_id/85148497236 |
op_rights |
open |
op_doi |
https://doi.org/10.1111/nph.18734 |
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New Phytologist |
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238 |
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6 |
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2577 |
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