Transforming growth factor-ß1b: a second TGF-ß1 paralogue in the rainbow trout (Oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation
13 pages, 9 figures, 2 tables The rainbow trout (Oncorhynchus mykiss) TGF-β1 sequence was one of the first fish cytokines described. Studies of its expression suggest it is constitutively expressed but displays refractory inducibility. Here we describe a second TGF-β1 (TGF-β1b) gene that is novel in...
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Online Access: | http://hdl.handle.net/10261/303511 https://doi.org/10.1016/j.fsi.2012.11.011 |
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ftcsic:oai:digital.csic.es:10261/303511 2024-02-11T10:02:10+01:00 Transforming growth factor-ß1b: a second TGF-ß1 paralogue in the rainbow trout (Oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation Maehr, Tanja Costa, M. M. González Vecino, José L. Wadsworth, Simon Martin, Samuel A. M. Wang, Tiehui Secombes, Christopher J. Marine Alliance for Science and Technology for Scotland 2013 http://hdl.handle.net/10261/303511 https://doi.org/10.1016/j.fsi.2012.11.011 en eng Elsevier https://doi.org/10.1016/j.fsi.2012.11.011 Sí Fish and Shellfish Immunology 34(2): 420-432 (2013) 1050-4648 http://hdl.handle.net/10261/303511 doi:10.1016/j.fsi.2012.11.011 2317-8261 none Rainbow trout TGF-β Macrophage LPS VHSV artículo 2013 ftcsic https://doi.org/10.1016/j.fsi.2012.11.011 2024-01-16T11:38:58Z 13 pages, 9 figures, 2 tables The rainbow trout (Oncorhynchus mykiss) TGF-β1 sequence was one of the first fish cytokines described. Studies of its expression suggest it is constitutively expressed but displays refractory inducibility. Here we describe a second TGF-β1 (TGF-β1b) gene that is novel in several respects. TGF-β1b possesses typical TGF-β features, including a CXC motif and an integrin binding site, a tetrabasic cut site and a mature peptide of 112 amino acids (aa) containing nine conserved cysteine residues. The mature peptide is 83% identical to the first TGF-β1 sequence described in rainbow trout, that we designate TGF-β1a, and relative to TGF-β1a shows higher homology to Atlantic salmon TGF-β1b, zebrafish TGF-β1a, and sea bass and seabream TGF-β1. The gene organisation of salmonid TGF-β1b genes, as inferred from Atlantic salmon whole genome shotgun contigs, is a 6 exon/5 intron structure with exons 3 and 4 of salmonid TGF-β1a genes apparently fused together. The two trout TGF-β1 genes have a wide distribution in vivo, with highest expression found in immune tissues for both isoforms indicating that TGF-β1 has a predominant role in immunity of fish. Expression of both genes was also seen during the ontogeny of trout, with TGF-β1a relatively constant in expression level but TGF-β1b increasing over time. Immune responses in head kidney (HK) macrophages induced by pathogen associated molecular patterns (PAMPs), pro-inflammatory cytokines, mitogens and pathway activators highly elevated the expression level of TGF-β1b but not that of TGF-β1a. TGF-β1b expression was also increased by polyinosinic:polycytidylic acid (poly(I:C)) and/or lipopolysaccharide (LPS) stimulation in three different trout cell lines studied. Finally we show that TGF-β1b is potentially involved in defense against infection with viral haemorrhagic septicemia virus (VHSV), which had no effect on TGF-β1a expression. Thus, it is likely the TGF-β1b gene represents a copy which fulfils the major immune orchestrating functions of TGF-β1 ... Article in Journal/Newspaper Atlantic salmon Digital.CSIC (Spanish National Research Council) Fish & Shellfish Immunology 34 2 420 432 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Rainbow trout TGF-β Macrophage LPS VHSV |
spellingShingle |
Rainbow trout TGF-β Macrophage LPS VHSV Maehr, Tanja Costa, M. M. González Vecino, José L. Wadsworth, Simon Martin, Samuel A. M. Wang, Tiehui Secombes, Christopher J. Transforming growth factor-ß1b: a second TGF-ß1 paralogue in the rainbow trout (Oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation |
topic_facet |
Rainbow trout TGF-β Macrophage LPS VHSV |
description |
13 pages, 9 figures, 2 tables The rainbow trout (Oncorhynchus mykiss) TGF-β1 sequence was one of the first fish cytokines described. Studies of its expression suggest it is constitutively expressed but displays refractory inducibility. Here we describe a second TGF-β1 (TGF-β1b) gene that is novel in several respects. TGF-β1b possesses typical TGF-β features, including a CXC motif and an integrin binding site, a tetrabasic cut site and a mature peptide of 112 amino acids (aa) containing nine conserved cysteine residues. The mature peptide is 83% identical to the first TGF-β1 sequence described in rainbow trout, that we designate TGF-β1a, and relative to TGF-β1a shows higher homology to Atlantic salmon TGF-β1b, zebrafish TGF-β1a, and sea bass and seabream TGF-β1. The gene organisation of salmonid TGF-β1b genes, as inferred from Atlantic salmon whole genome shotgun contigs, is a 6 exon/5 intron structure with exons 3 and 4 of salmonid TGF-β1a genes apparently fused together. The two trout TGF-β1 genes have a wide distribution in vivo, with highest expression found in immune tissues for both isoforms indicating that TGF-β1 has a predominant role in immunity of fish. Expression of both genes was also seen during the ontogeny of trout, with TGF-β1a relatively constant in expression level but TGF-β1b increasing over time. Immune responses in head kidney (HK) macrophages induced by pathogen associated molecular patterns (PAMPs), pro-inflammatory cytokines, mitogens and pathway activators highly elevated the expression level of TGF-β1b but not that of TGF-β1a. TGF-β1b expression was also increased by polyinosinic:polycytidylic acid (poly(I:C)) and/or lipopolysaccharide (LPS) stimulation in three different trout cell lines studied. Finally we show that TGF-β1b is potentially involved in defense against infection with viral haemorrhagic septicemia virus (VHSV), which had no effect on TGF-β1a expression. Thus, it is likely the TGF-β1b gene represents a copy which fulfils the major immune orchestrating functions of TGF-β1 ... |
author2 |
Marine Alliance for Science and Technology for Scotland |
format |
Article in Journal/Newspaper |
author |
Maehr, Tanja Costa, M. M. González Vecino, José L. Wadsworth, Simon Martin, Samuel A. M. Wang, Tiehui Secombes, Christopher J. |
author_facet |
Maehr, Tanja Costa, M. M. González Vecino, José L. Wadsworth, Simon Martin, Samuel A. M. Wang, Tiehui Secombes, Christopher J. |
author_sort |
Maehr, Tanja |
title |
Transforming growth factor-ß1b: a second TGF-ß1 paralogue in the rainbow trout (Oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation |
title_short |
Transforming growth factor-ß1b: a second TGF-ß1 paralogue in the rainbow trout (Oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation |
title_full |
Transforming growth factor-ß1b: a second TGF-ß1 paralogue in the rainbow trout (Oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation |
title_fullStr |
Transforming growth factor-ß1b: a second TGF-ß1 paralogue in the rainbow trout (Oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation |
title_full_unstemmed |
Transforming growth factor-ß1b: a second TGF-ß1 paralogue in the rainbow trout (Oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation |
title_sort |
transforming growth factor-ß1b: a second tgf-ß1 paralogue in the rainbow trout (oncorhynchus mykiss) that has a lower constitutive expression but is more responsive to immune stimulation |
publisher |
Elsevier |
publishDate |
2013 |
url |
http://hdl.handle.net/10261/303511 https://doi.org/10.1016/j.fsi.2012.11.011 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://doi.org/10.1016/j.fsi.2012.11.011 Sí Fish and Shellfish Immunology 34(2): 420-432 (2013) 1050-4648 http://hdl.handle.net/10261/303511 doi:10.1016/j.fsi.2012.11.011 2317-8261 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.fsi.2012.11.011 |
container_title |
Fish & Shellfish Immunology |
container_volume |
34 |
container_issue |
2 |
container_start_page |
420 |
op_container_end_page |
432 |
_version_ |
1790598070040264704 |