Semen cryopreservation in black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguins Effects of thawing temperature on semen characteristics
In this study, there was an examination of the effect on the characteristics of cryopreserved black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguin semen, of thawing at 37 and 5 °C. For two consecutive years, semen was collected and frozen during the April-June period from six gen...
Published in: | Animal Reproduction Science |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2019
|
Subjects: | |
Online Access: | https://hdl.handle.net/20.500.12792/772 http://hdl.handle.net/10261/290655 https://doi.org/10.1016/j.anireprosci.2018.11.011 |
id |
ftcsic:oai:digital.csic.es:10261/290655 |
---|---|
record_format |
openpolar |
spelling |
ftcsic:oai:digital.csic.es:10261/290655 2024-02-11T10:03:58+01:00 Semen cryopreservation in black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguins Effects of thawing temperature on semen characteristics Santiago Moreno, Julián Castaño García, Cristina Toledano Díaz, Adolfo Esteso Diez, Milagros Cristina Martínez-Nevado, E. Gimeno-Martínez, J. López-Goya, A. 2019 https://hdl.handle.net/20.500.12792/772 http://hdl.handle.net/10261/290655 https://doi.org/10.1016/j.anireprosci.2018.11.011 en eng Elsevier Animal Reproduction Science 200: 60-66 (2019) 0378-4320 http://hdl.handle.net/20.500.12792/772 http://hdl.handle.net/10261/290655 doi:10.1016/j.anireprosci.2018.11.011 none Freezing-thawing Penguin Sperm Temperature artículo 2019 ftcsic https://doi.org/20.500.12792/77210.1016/j.anireprosci.2018.11.011 2024-01-16T11:35:11Z In this study, there was an examination of the effect on the characteristics of cryopreserved black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguin semen, of thawing at 37 and 5 °C. For two consecutive years, semen was collected and frozen during the April-June period from six gentoo penguins, and during the October-November period from 13 black-footed penguins. After thawing, sperm motility variables were examined by computer-assisted sperm analysis. Propidium iodide and SYBR-14 were used as fluorochromes for the examination of membrane integrity. For the gentoo penguins, no differences were detected in the values of frozen-thawed semen characteristics after thawing at 37 or 5 °C. For the black-footed penguins, however, thawing at 5 °C resulted in greater values (P < 0.05) for straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), straightness (STR), and wobble (WOB) as compared with thawing at 37 °C. After thawing at 37 ºC, there were greater values with gentoo penguin sperm for percentage motile sperm, progressive motility, curvilinear velocity (VCL), VSL VAP, LIN, STR, WOB and beat-cross frequency (BCF; P < 0.05) than that for black-footed penguin sperm. After thawing at 5 ºC, there were no differences in values for any variables between the two species. In conclusion, thawing temperature affects semen characteristics in a species-specific manner. The present data strongly suggest that cryopreservation procedures should be adapted for use with each penguin species. Cryopreserved black-footed penguin semen should be thawed after cryopreservation at 5 ºC, while that of gentoo penguins can be thawed at either 5 or 37 ºC. Article in Journal/Newspaper Gentoo penguin Pygoscelis papua Digital.CSIC (Spanish National Research Council) Animal Reproduction Science 200 60 66 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Freezing-thawing Penguin Sperm Temperature |
spellingShingle |
Freezing-thawing Penguin Sperm Temperature Santiago Moreno, Julián Castaño García, Cristina Toledano Díaz, Adolfo Esteso Diez, Milagros Cristina Martínez-Nevado, E. Gimeno-Martínez, J. López-Goya, A. Semen cryopreservation in black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguins Effects of thawing temperature on semen characteristics |
topic_facet |
Freezing-thawing Penguin Sperm Temperature |
description |
In this study, there was an examination of the effect on the characteristics of cryopreserved black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguin semen, of thawing at 37 and 5 °C. For two consecutive years, semen was collected and frozen during the April-June period from six gentoo penguins, and during the October-November period from 13 black-footed penguins. After thawing, sperm motility variables were examined by computer-assisted sperm analysis. Propidium iodide and SYBR-14 were used as fluorochromes for the examination of membrane integrity. For the gentoo penguins, no differences were detected in the values of frozen-thawed semen characteristics after thawing at 37 or 5 °C. For the black-footed penguins, however, thawing at 5 °C resulted in greater values (P < 0.05) for straight-line velocity (VSL), average path velocity (VAP), linearity (LIN), straightness (STR), and wobble (WOB) as compared with thawing at 37 °C. After thawing at 37 ºC, there were greater values with gentoo penguin sperm for percentage motile sperm, progressive motility, curvilinear velocity (VCL), VSL VAP, LIN, STR, WOB and beat-cross frequency (BCF; P < 0.05) than that for black-footed penguin sperm. After thawing at 5 ºC, there were no differences in values for any variables between the two species. In conclusion, thawing temperature affects semen characteristics in a species-specific manner. The present data strongly suggest that cryopreservation procedures should be adapted for use with each penguin species. Cryopreserved black-footed penguin semen should be thawed after cryopreservation at 5 ºC, while that of gentoo penguins can be thawed at either 5 or 37 ºC. |
format |
Article in Journal/Newspaper |
author |
Santiago Moreno, Julián Castaño García, Cristina Toledano Díaz, Adolfo Esteso Diez, Milagros Cristina Martínez-Nevado, E. Gimeno-Martínez, J. López-Goya, A. |
author_facet |
Santiago Moreno, Julián Castaño García, Cristina Toledano Díaz, Adolfo Esteso Diez, Milagros Cristina Martínez-Nevado, E. Gimeno-Martínez, J. López-Goya, A. |
author_sort |
Santiago Moreno, Julián |
title |
Semen cryopreservation in black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguins Effects of thawing temperature on semen characteristics |
title_short |
Semen cryopreservation in black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguins Effects of thawing temperature on semen characteristics |
title_full |
Semen cryopreservation in black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguins Effects of thawing temperature on semen characteristics |
title_fullStr |
Semen cryopreservation in black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguins Effects of thawing temperature on semen characteristics |
title_full_unstemmed |
Semen cryopreservation in black-footed (Spheniscus demersus) and gentoo (Pygoscelis papua) penguins Effects of thawing temperature on semen characteristics |
title_sort |
semen cryopreservation in black-footed (spheniscus demersus) and gentoo (pygoscelis papua) penguins effects of thawing temperature on semen characteristics |
publisher |
Elsevier |
publishDate |
2019 |
url |
https://hdl.handle.net/20.500.12792/772 http://hdl.handle.net/10261/290655 https://doi.org/10.1016/j.anireprosci.2018.11.011 |
genre |
Gentoo penguin Pygoscelis papua |
genre_facet |
Gentoo penguin Pygoscelis papua |
op_relation |
Animal Reproduction Science 200: 60-66 (2019) 0378-4320 http://hdl.handle.net/20.500.12792/772 http://hdl.handle.net/10261/290655 doi:10.1016/j.anireprosci.2018.11.011 |
op_rights |
none |
op_doi |
https://doi.org/20.500.12792/77210.1016/j.anireprosci.2018.11.011 |
container_title |
Animal Reproduction Science |
container_volume |
200 |
container_start_page |
60 |
op_container_end_page |
66 |
_version_ |
1790600371278708736 |