Protection and antibody response induced by intramuscular DNA vaccine encoding for viral haemorrhagic septicaemia virus (VHSV) G glycoprotein in turbot (Scophthalmus maximus)

Turbot (Scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in Europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (VHSV) strains. To evaluate the possibility of controlling the outbreaks of this infectious disease by means of...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Pereiro, P., Martinez-Lopez, A., Falco, A., Dios, S., Figueras, A., Coll, J. M., Novoa, B., Estepa, A.
Format: Article in Journal/Newspaper
Language:English
Published: Elsevier 2012
Subjects:
DNA vaccine
VHSV
Turbot
Glycoprotein G
Rhabdovirus
Scophthalmus maximus
Online Access:https://hdl.handle.net/20.500.12792/3387
http://hdl.handle.net/10261/290518
https://doi.org/10.1016/j.fsi.2012.03.004
Description
Summary:Turbot (Scophthalmus maximus) is a high-value farmed marine flatfish with growing demand and production levels in Europe susceptible to turbot-specific viral haemorrhagic septicaemia virus (VHSV) strains. To evaluate the possibility of controlling the outbreaks of this infectious disease by means of DNA vaccination, the gpG of a VHSV isolated from farmed turbot (VHSV 860) was cloned into an expression plasmid containing the human cytomegalovirus (CMV) promoter (pMCV1.4-G 860). In our experimental conditions, DNA immunised turbots were more than 85% protected against VHSV 860 lethal challenge and showed both VHSV-gpG specific and neutralizing antibodies. To our knowledge this is the first report showing the efficacy of turbot genetic immunisation against a VHSV. Work is in progress to determine the contribution of innate and adaptive immunity to the protective response elicited by the immunization. © 2012.

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