West Nile Virus Lineage 2 Spreads Westwards in Europe and Overwinters in North-Eastern Spain (2017-2020)
West Nile virus lineage 2 (WNV-L2) emerged in Europe in 2004; since then, it has spread across the continent, causing outbreaks in humans and animals. During 2017 and 2020, WNV-L2 was detected and isolated from four northern goshawks in two provinces of Catalonia (north eastern Spain). In order to c...
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ftcsic:oai:digital.csic.es:10261/289656 2024-02-11T10:07:07+01:00 West Nile Virus Lineage 2 Spreads Westwards in Europe and Overwinters in North-Eastern Spain (2017-2020) Aguilera-Sepúlveda, Pilar Napp, Sebastián Llorente, Francisco Solano-Manrique, Carlos Molina-López, Rafael Obón, Elena Solé, Alba Jiménez-Clavero, Miguel Ángel Fernández-Pinero, Jovita Busquets, Núria 2022-03-09 https://hdl.handle.net/20.500.12792/6187 http://hdl.handle.net/10261/289656 https://doi.org/10.3390/v14030569 en eng Multidisciplinary Digital Publishing Institute Viruses 14 (3): 569 (2022) 1999-4915 http://hdl.handle.net/20.500.12792/6187 http://hdl.handle.net/10261/289656 doi:10.3390/v14030569 open Europe Spain West Nile virus Avian host Lineage-2 Northern goshawk Overwintering Phylogeny artículo 2022 ftcsic https://doi.org/20.500.12792/618710.3390/v14030569 2024-01-16T11:34:31Z West Nile virus lineage 2 (WNV-L2) emerged in Europe in 2004; since then, it has spread across the continent, causing outbreaks in humans and animals. During 2017 and 2020, WNV-L2 was detected and isolated from four northern goshawks in two provinces of Catalonia (north eastern Spain). In order to characterise the first Spanish WNV-L2 isolates and elucidate the potential overwintering of the virus in this Mediterranean region, complete genome sequencing, phylogenetic analyses, and a study of phenotypic characterisation were performed. Our results showed that these Spanish isolates belonged to the central-southern WNV-L2 clade. In more detail, they were related to the Lombardy cluster that emerged in Italy in 2013 and has been able to spread westwards, causing outbreaks in France (2018) and Spain (2017 and 2020). Phenotypic characterisation performed in vitro showed that these isolates presented characteristics corresponding to strains of moderate to high virulence. All these findings evidence that these WNV-L2 strains have been able to circulate and overwinter in the region, and are pathogenic, at least in northern goshawks, which seem to be very susceptible to WNV infection and may be good indicators of WNV-L2 circulation. Due to the increasing number of human and animal cases in Europe in the last years, this zoonotic flavivirus should be kept under extensive surveillance, following a One-Health approach. Article in Journal/Newspaper Northern Goshawk Digital.CSIC (Spanish National Research Council) Viruses 14 3 569 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Europe Spain West Nile virus Avian host Lineage-2 Northern goshawk Overwintering Phylogeny |
spellingShingle |
Europe Spain West Nile virus Avian host Lineage-2 Northern goshawk Overwintering Phylogeny Aguilera-Sepúlveda, Pilar Napp, Sebastián Llorente, Francisco Solano-Manrique, Carlos Molina-López, Rafael Obón, Elena Solé, Alba Jiménez-Clavero, Miguel Ángel Fernández-Pinero, Jovita Busquets, Núria West Nile Virus Lineage 2 Spreads Westwards in Europe and Overwinters in North-Eastern Spain (2017-2020) |
topic_facet |
Europe Spain West Nile virus Avian host Lineage-2 Northern goshawk Overwintering Phylogeny |
description |
West Nile virus lineage 2 (WNV-L2) emerged in Europe in 2004; since then, it has spread across the continent, causing outbreaks in humans and animals. During 2017 and 2020, WNV-L2 was detected and isolated from four northern goshawks in two provinces of Catalonia (north eastern Spain). In order to characterise the first Spanish WNV-L2 isolates and elucidate the potential overwintering of the virus in this Mediterranean region, complete genome sequencing, phylogenetic analyses, and a study of phenotypic characterisation were performed. Our results showed that these Spanish isolates belonged to the central-southern WNV-L2 clade. In more detail, they were related to the Lombardy cluster that emerged in Italy in 2013 and has been able to spread westwards, causing outbreaks in France (2018) and Spain (2017 and 2020). Phenotypic characterisation performed in vitro showed that these isolates presented characteristics corresponding to strains of moderate to high virulence. All these findings evidence that these WNV-L2 strains have been able to circulate and overwinter in the region, and are pathogenic, at least in northern goshawks, which seem to be very susceptible to WNV infection and may be good indicators of WNV-L2 circulation. Due to the increasing number of human and animal cases in Europe in the last years, this zoonotic flavivirus should be kept under extensive surveillance, following a One-Health approach. |
format |
Article in Journal/Newspaper |
author |
Aguilera-Sepúlveda, Pilar Napp, Sebastián Llorente, Francisco Solano-Manrique, Carlos Molina-López, Rafael Obón, Elena Solé, Alba Jiménez-Clavero, Miguel Ángel Fernández-Pinero, Jovita Busquets, Núria |
author_facet |
Aguilera-Sepúlveda, Pilar Napp, Sebastián Llorente, Francisco Solano-Manrique, Carlos Molina-López, Rafael Obón, Elena Solé, Alba Jiménez-Clavero, Miguel Ángel Fernández-Pinero, Jovita Busquets, Núria |
author_sort |
Aguilera-Sepúlveda, Pilar |
title |
West Nile Virus Lineage 2 Spreads Westwards in Europe and Overwinters in North-Eastern Spain (2017-2020) |
title_short |
West Nile Virus Lineage 2 Spreads Westwards in Europe and Overwinters in North-Eastern Spain (2017-2020) |
title_full |
West Nile Virus Lineage 2 Spreads Westwards in Europe and Overwinters in North-Eastern Spain (2017-2020) |
title_fullStr |
West Nile Virus Lineage 2 Spreads Westwards in Europe and Overwinters in North-Eastern Spain (2017-2020) |
title_full_unstemmed |
West Nile Virus Lineage 2 Spreads Westwards in Europe and Overwinters in North-Eastern Spain (2017-2020) |
title_sort |
west nile virus lineage 2 spreads westwards in europe and overwinters in north-eastern spain (2017-2020) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://hdl.handle.net/20.500.12792/6187 http://hdl.handle.net/10261/289656 https://doi.org/10.3390/v14030569 |
genre |
Northern Goshawk |
genre_facet |
Northern Goshawk |
op_relation |
Viruses 14 (3): 569 (2022) 1999-4915 http://hdl.handle.net/20.500.12792/6187 http://hdl.handle.net/10261/289656 doi:10.3390/v14030569 |
op_rights |
open |
op_doi |
https://doi.org/20.500.12792/618710.3390/v14030569 |
container_title |
Viruses |
container_volume |
14 |
container_issue |
3 |
container_start_page |
569 |
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1790605272834637824 |