West Nile and Usutu virus infections in wild birds admitted to rehabilitation centres in Extremadura, western Spain, 2017-2019
10 Pág. Centro de Investigación en Sanidad Animal (CISA) West Nile virus (WNV) is an emerging flavivirus transmitted generally by mosquitoes of Culex genus. It is maintained in an enzootic life cycle where birds act as reservoir hosts. Humans and horses are also susceptible to infection, and occasio...
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ftcsic:oai:digital.csic.es:10261/287628 2024-06-23T07:52:34+00:00 West Nile and Usutu virus infections in wild birds admitted to rehabilitation centres in Extremadura, western Spain, 2017-2019 Bravo, Daniel Aguilera-Sepúlveda, Pilar Guerrero-Carvajal, Fátima Llorente, Francisco Reina, David Pérez-Martín, Juan Enrique Jiménez-Clavero, Miguel Ángel Frontera, Eva Junta de Extremadura European Commission CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Bravo, Daniel Aguilera-Sepúlveda, Pilar Guerrero-Carvajal, Fátima Llorente, Francisco Pérez-Martín, J Enrique Jiménez-Clavero, Miguel Ángel Frontera, Eva 2021-04 http://hdl.handle.net/10261/287628 https://doi.org/10.1016/j.vetmic.2021.109020 https://doi.org/10.13039/501100014181 https://doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85101914721 en eng Elsevier #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/IB16135 info:eu-repo/grantAgreement/EC/GR18148 info:eu-repo/grantAgreement/INIA-CISA//E-RTA2015-00002-C02-00 Veterinary microbiology Publisher's version https://doi.org/10.1016/j.vetmic.2021.109020 Sí Veterinary Microbiology 255: 2021 Article number 0378-1135 http://hdl.handle.net/10261/287628 doi:10.1016/j.vetmic.2021.109020 http://dx.doi.org/10.13039/501100014181 http://dx.doi.org/10.13039/501100000780 33677369 2-s2.0-85101914721 https://api.elsevier.com/content/abstract/scopus_id/85101914721 open Extremadura Seroprevalence Spain Usutu virus West Nile virus artículo http://purl.org/coar/resource_type/c_6501 2021 ftcsic https://doi.org/10.1016/j.vetmic.2021.10902010.13039/50110001418110.13039/501100000780 2024-05-29T00:01:24Z 10 Pág. Centro de Investigación en Sanidad Animal (CISA) West Nile virus (WNV) is an emerging flavivirus transmitted generally by mosquitoes of Culex genus. It is maintained in an enzootic life cycle where birds act as reservoir hosts. Humans and horses are also susceptible to infection, and occasionally, they suffer from neurological complications. However, they do not transmit the virus to other vectors, behaving as dead-end hosts. Sporadic WNV outbreaks observed in horses and wild birds from Extremadura (western Spain) during 2016 and 2017 seasons prompted to carry out this survey in wild birds, focused on specimens coming from two wildlife rehabilitation centres. Between October 2017 and December 2019, samples from 391 wild birds, belonging to 56 different species were collected and analysed in search of evidence of WNV infection. The analysis of serum samples for WNV-specific antibodies by ELISA, whose specificity was subsequently confirmed by virus-neutralisation test (VNT) showed positive results in 18.23 % birds belonging to 18 different species. Pelecaniformes (33.33 %), Accipitriformes (25.77 %) and Strigiformes (22.92 %) orders had the higher seroprevalences. Remarkably, WNV-specific antibodies were found in a black stork for the first time in Europe. Analysis by real time RT-PCR in symptomatic birds confirmed the presence of WNV lineage 1 RNA in griffon vulture and little owls. Specificity analysis of ELISA positive and doubtful sera was performed by differential VNT titration against WNV and two other cross-reacting avian flaviviruses found in Spain: Usutu virus (USUV) and Bagaza virus (BAGV). Only four samples showed USUV-specific antibodies (1.04 %) corresponding to three species: Eurasian eagle-owl, griffon vulture and great bustard (first detection in Europe) whereas no samples were found reactive to BAGV. Differential VNT yielded undetermined flavivirus result in 16 samples (4.17 %). This is the first study carried out on wild birds from Extremadura (western Spain). It highlights the ... Article in Journal/Newspaper eurasian eagle-owl Digital.CSIC (Spanish National Research Council) Veterinary Microbiology 255 109020 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Extremadura Seroprevalence Spain Usutu virus West Nile virus |
spellingShingle |
Extremadura Seroprevalence Spain Usutu virus West Nile virus Bravo, Daniel Aguilera-Sepúlveda, Pilar Guerrero-Carvajal, Fátima Llorente, Francisco Reina, David Pérez-Martín, Juan Enrique Jiménez-Clavero, Miguel Ángel Frontera, Eva West Nile and Usutu virus infections in wild birds admitted to rehabilitation centres in Extremadura, western Spain, 2017-2019 |
topic_facet |
Extremadura Seroprevalence Spain Usutu virus West Nile virus |
description |
10 Pág. Centro de Investigación en Sanidad Animal (CISA) West Nile virus (WNV) is an emerging flavivirus transmitted generally by mosquitoes of Culex genus. It is maintained in an enzootic life cycle where birds act as reservoir hosts. Humans and horses are also susceptible to infection, and occasionally, they suffer from neurological complications. However, they do not transmit the virus to other vectors, behaving as dead-end hosts. Sporadic WNV outbreaks observed in horses and wild birds from Extremadura (western Spain) during 2016 and 2017 seasons prompted to carry out this survey in wild birds, focused on specimens coming from two wildlife rehabilitation centres. Between October 2017 and December 2019, samples from 391 wild birds, belonging to 56 different species were collected and analysed in search of evidence of WNV infection. The analysis of serum samples for WNV-specific antibodies by ELISA, whose specificity was subsequently confirmed by virus-neutralisation test (VNT) showed positive results in 18.23 % birds belonging to 18 different species. Pelecaniformes (33.33 %), Accipitriformes (25.77 %) and Strigiformes (22.92 %) orders had the higher seroprevalences. Remarkably, WNV-specific antibodies were found in a black stork for the first time in Europe. Analysis by real time RT-PCR in symptomatic birds confirmed the presence of WNV lineage 1 RNA in griffon vulture and little owls. Specificity analysis of ELISA positive and doubtful sera was performed by differential VNT titration against WNV and two other cross-reacting avian flaviviruses found in Spain: Usutu virus (USUV) and Bagaza virus (BAGV). Only four samples showed USUV-specific antibodies (1.04 %) corresponding to three species: Eurasian eagle-owl, griffon vulture and great bustard (first detection in Europe) whereas no samples were found reactive to BAGV. Differential VNT yielded undetermined flavivirus result in 16 samples (4.17 %). This is the first study carried out on wild birds from Extremadura (western Spain). It highlights the ... |
author2 |
Junta de Extremadura European Commission CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Bravo, Daniel Aguilera-Sepúlveda, Pilar Guerrero-Carvajal, Fátima Llorente, Francisco Pérez-Martín, J Enrique Jiménez-Clavero, Miguel Ángel Frontera, Eva |
format |
Article in Journal/Newspaper |
author |
Bravo, Daniel Aguilera-Sepúlveda, Pilar Guerrero-Carvajal, Fátima Llorente, Francisco Reina, David Pérez-Martín, Juan Enrique Jiménez-Clavero, Miguel Ángel Frontera, Eva |
author_facet |
Bravo, Daniel Aguilera-Sepúlveda, Pilar Guerrero-Carvajal, Fátima Llorente, Francisco Reina, David Pérez-Martín, Juan Enrique Jiménez-Clavero, Miguel Ángel Frontera, Eva |
author_sort |
Bravo, Daniel |
title |
West Nile and Usutu virus infections in wild birds admitted to rehabilitation centres in Extremadura, western Spain, 2017-2019 |
title_short |
West Nile and Usutu virus infections in wild birds admitted to rehabilitation centres in Extremadura, western Spain, 2017-2019 |
title_full |
West Nile and Usutu virus infections in wild birds admitted to rehabilitation centres in Extremadura, western Spain, 2017-2019 |
title_fullStr |
West Nile and Usutu virus infections in wild birds admitted to rehabilitation centres in Extremadura, western Spain, 2017-2019 |
title_full_unstemmed |
West Nile and Usutu virus infections in wild birds admitted to rehabilitation centres in Extremadura, western Spain, 2017-2019 |
title_sort |
west nile and usutu virus infections in wild birds admitted to rehabilitation centres in extremadura, western spain, 2017-2019 |
publisher |
Elsevier |
publishDate |
2021 |
url |
http://hdl.handle.net/10261/287628 https://doi.org/10.1016/j.vetmic.2021.109020 https://doi.org/10.13039/501100014181 https://doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85101914721 |
genre |
eurasian eagle-owl |
genre_facet |
eurasian eagle-owl |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/IB16135 info:eu-repo/grantAgreement/EC/GR18148 info:eu-repo/grantAgreement/INIA-CISA//E-RTA2015-00002-C02-00 Veterinary microbiology Publisher's version https://doi.org/10.1016/j.vetmic.2021.109020 Sí Veterinary Microbiology 255: 2021 Article number 0378-1135 http://hdl.handle.net/10261/287628 doi:10.1016/j.vetmic.2021.109020 http://dx.doi.org/10.13039/501100014181 http://dx.doi.org/10.13039/501100000780 33677369 2-s2.0-85101914721 https://api.elsevier.com/content/abstract/scopus_id/85101914721 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.vetmic.2021.10902010.13039/50110001418110.13039/501100000780 |
container_title |
Veterinary Microbiology |
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255 |
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109020 |
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