Characterization of the turbot (Scophthalmus maximus) interleukin-18: Identification of splicing variants, phylogeny, synteny and expression analysis
Poster.-- 4th Congress of the International Society of Fish & Shellfish Immunology, December 12-15, 2022, Bode, Norway Interleukin-18 (IL-18) is a pro-inflammatory cytokine that belongs to the interleukin-1 (IL-1) family of cytokines. As occurs with IL-1 β. it is synthetized as an inactive precu...
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ftcsic:oai:digital.csic.es:10261/285130 2024-02-11T10:08:28+01:00 Characterization of the turbot (Scophthalmus maximus) interleukin-18: Identification of splicing variants, phylogeny, synteny and expression analysis Pereiro, Patricia Lama, R. Figueras Huerta, Antonio Novoa, Beatriz Ministerio de Economía, Industria y Competitividad (España) Xunta de Galicia Axencia Galega de Innovación 2022 http://hdl.handle.net/10261/285130 https://doi.org/10.13039/501100010769 https://doi.org/10.13039/501100010198 https://doi.org/10.13039/501100010801 en eng Sí Congress of the International Society of Fish & Shellfish Immunology (2022) http://hdl.handle.net/10261/285130 http://dx.doi.org/10.13039/501100010769 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100010801 none Intlnterleukin-18 Teleost Turbot Splicing variants Immune response póster de congreso 2022 ftcsic https://doi.org/10.13039/50110001076910.13039/50110001019810.13039/501100010801 2024-01-16T11:32:11Z Poster.-- 4th Congress of the International Society of Fish & Shellfish Immunology, December 12-15, 2022, Bode, Norway Interleukin-18 (IL-18) is a pro-inflammatory cytokine that belongs to the interleukin-1 (IL-1) family of cytokines. As occurs with IL-1 β. it is synthetized as an inactive precursor peptide that is mainly processed by the cysteine protease caspase-1 in the inflammasome complex. In mammals, and in collaboration with IL-12, it has been described as an important cytokine controlling the Thl-mediated immune responses through the induction of IFN-y. Although its function in mammals is well stablished, the activity of this cytokine in teleost remains to be elucidated. This could be due, among other things, to the absence of this gene in the fish model species zebrafish, but also to its complex regulation. As it was observed for rainbow trout and human, il18 splicing variants were also found in turbot, which could represent a regulatory mechanism of its bioactivity. In the case of turbot, three splicing variants were observed (SVl-3), and one of them showed an insertion of 10 amino acids in the middle of the potential caspase-1 cleavage position, reflecting that this is probably a form resistant to the processing by the inflammasome. Phylogenetic and threedimensional analyses of turbot iI18 revealed that it is relatively wellconserved in vertebrates, although only a partial conservation of the gene synteny was observed between fish and mammals. As it was expected, turbot il18 splicing variants were mainly expressed in immune tissues under healthy conditions, and their expression was induced by a bacterial challenge, although certain inhibitions were observed after viraland parasitic infections. In the case of the viral challenge, il18 downregulations did not seem to be due to the effect of type I IFNs This work was financially supported by the Ministerio de Economia, Industria y Competitividad of Spain (BIO2017-82851-0-2-R). Our laboratory is funded by the Interreg VA Spain-Portugal cooperation ... Still Image Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Norway |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Intlnterleukin-18 Teleost Turbot Splicing variants Immune response |
spellingShingle |
Intlnterleukin-18 Teleost Turbot Splicing variants Immune response Pereiro, Patricia Lama, R. Figueras Huerta, Antonio Novoa, Beatriz Characterization of the turbot (Scophthalmus maximus) interleukin-18: Identification of splicing variants, phylogeny, synteny and expression analysis |
topic_facet |
Intlnterleukin-18 Teleost Turbot Splicing variants Immune response |
description |
Poster.-- 4th Congress of the International Society of Fish & Shellfish Immunology, December 12-15, 2022, Bode, Norway Interleukin-18 (IL-18) is a pro-inflammatory cytokine that belongs to the interleukin-1 (IL-1) family of cytokines. As occurs with IL-1 β. it is synthetized as an inactive precursor peptide that is mainly processed by the cysteine protease caspase-1 in the inflammasome complex. In mammals, and in collaboration with IL-12, it has been described as an important cytokine controlling the Thl-mediated immune responses through the induction of IFN-y. Although its function in mammals is well stablished, the activity of this cytokine in teleost remains to be elucidated. This could be due, among other things, to the absence of this gene in the fish model species zebrafish, but also to its complex regulation. As it was observed for rainbow trout and human, il18 splicing variants were also found in turbot, which could represent a regulatory mechanism of its bioactivity. In the case of turbot, three splicing variants were observed (SVl-3), and one of them showed an insertion of 10 amino acids in the middle of the potential caspase-1 cleavage position, reflecting that this is probably a form resistant to the processing by the inflammasome. Phylogenetic and threedimensional analyses of turbot iI18 revealed that it is relatively wellconserved in vertebrates, although only a partial conservation of the gene synteny was observed between fish and mammals. As it was expected, turbot il18 splicing variants were mainly expressed in immune tissues under healthy conditions, and their expression was induced by a bacterial challenge, although certain inhibitions were observed after viraland parasitic infections. In the case of the viral challenge, il18 downregulations did not seem to be due to the effect of type I IFNs This work was financially supported by the Ministerio de Economia, Industria y Competitividad of Spain (BIO2017-82851-0-2-R). Our laboratory is funded by the Interreg VA Spain-Portugal cooperation ... |
author2 |
Ministerio de Economía, Industria y Competitividad (España) Xunta de Galicia Axencia Galega de Innovación |
format |
Still Image |
author |
Pereiro, Patricia Lama, R. Figueras Huerta, Antonio Novoa, Beatriz |
author_facet |
Pereiro, Patricia Lama, R. Figueras Huerta, Antonio Novoa, Beatriz |
author_sort |
Pereiro, Patricia |
title |
Characterization of the turbot (Scophthalmus maximus) interleukin-18: Identification of splicing variants, phylogeny, synteny and expression analysis |
title_short |
Characterization of the turbot (Scophthalmus maximus) interleukin-18: Identification of splicing variants, phylogeny, synteny and expression analysis |
title_full |
Characterization of the turbot (Scophthalmus maximus) interleukin-18: Identification of splicing variants, phylogeny, synteny and expression analysis |
title_fullStr |
Characterization of the turbot (Scophthalmus maximus) interleukin-18: Identification of splicing variants, phylogeny, synteny and expression analysis |
title_full_unstemmed |
Characterization of the turbot (Scophthalmus maximus) interleukin-18: Identification of splicing variants, phylogeny, synteny and expression analysis |
title_sort |
characterization of the turbot (scophthalmus maximus) interleukin-18: identification of splicing variants, phylogeny, synteny and expression analysis |
publishDate |
2022 |
url |
http://hdl.handle.net/10261/285130 https://doi.org/10.13039/501100010769 https://doi.org/10.13039/501100010198 https://doi.org/10.13039/501100010801 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
Sí Congress of the International Society of Fish & Shellfish Immunology (2022) http://hdl.handle.net/10261/285130 http://dx.doi.org/10.13039/501100010769 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100010801 |
op_rights |
none |
op_doi |
https://doi.org/10.13039/50110001076910.13039/50110001019810.13039/501100010801 |
_version_ |
1790607810868805632 |