Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica
The three-dimensional structures of two types of cyanobacterium-dominated microbial mats from meltwater ponds on the McMurdo Ice Shelf were as determined by using a broad suite of complementary techniques, including optical and fluorescence microscopy, confocal scanning laser microscopy, scanning el...
Published in: | Applied and Environmental Microbiology |
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American Society for Microbiology
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Online Access: | http://hdl.handle.net/10261/2842 https://doi.org/10.1128/AEM.70.1.569-580.2004 |
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ftcsic:oai:digital.csic.es:10261/2842 2024-02-11T09:56:10+01:00 Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica Ríos, Asunción de los Ascaso, Carmen Wierzchos, Jacek Fernández-Valiente, Eduardo Quesada, Antonio 2004-01 5313479 bytes application/pdf http://hdl.handle.net/10261/2842 https://doi.org/10.1128/AEM.70.1.569-580.2004 en eng American Society for Microbiology Appl Environ Microbiol. 2004 January; 70(1): 569–580. 0099-2240 http://hdl.handle.net/10261/2842 doi:10.1128/AEM.70.1.569-580.2004 14711689 open artículo http://purl.org/coar/resource_type/c_6501 2004 ftcsic https://doi.org/10.1128/AEM.70.1.569-580.2004 2024-01-16T09:18:51Z The three-dimensional structures of two types of cyanobacterium-dominated microbial mats from meltwater ponds on the McMurdo Ice Shelf were as determined by using a broad suite of complementary techniques, including optical and fluorescence microscopy, confocal scanning laser microscopy, scanning electron microscopy with back-scattered electron-imaging mode, low-temperature scanning electron microscopy, and microanalyitical X-ray energy dispersive spectroscopy. By using a combination of the different in situ microscopic techniques, the Antarctic microbial mats were found to be structures with vertical stratification of groups of cyanobacteria and mineral sediments, high contents of extracellular polymeric substances, and large void spaces occupied by water. In cyanobacterium-rich layers, heterocystous nostocalean and nonheterocystous oscillatorialean taxa were the most abundant taxa and appeared to be intermixed with fine-size deposits of epicellular silica and calcium carbonate. Most of the cyanobacterial filaments had similar orientations in zones without sediment particles, but thin filaments were tangled among thicker filaments. The combination of the microscopic techniques used showed the relative positions of biological and mineral entities within the microbial mats and enabled some speculation about their interactions. This work was supported by grants BOS2000-1121, REN2002-03542, ANT96-2174-E, ANT99-1319-E, and REN2000-0435-ANT. Peer reviewed Article in Journal/Newspaper Antarc* Antarctic Antarctica Ice Shelf McMurdo Ice Shelf Digital.CSIC (Spanish National Research Council) Antarctic McMurdo Ice Shelf ENVELOPE(166.500,166.500,-78.000,-78.000) The Antarctic Applied and Environmental Microbiology 70 1 569 580 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
language |
English |
description |
The three-dimensional structures of two types of cyanobacterium-dominated microbial mats from meltwater ponds on the McMurdo Ice Shelf were as determined by using a broad suite of complementary techniques, including optical and fluorescence microscopy, confocal scanning laser microscopy, scanning electron microscopy with back-scattered electron-imaging mode, low-temperature scanning electron microscopy, and microanalyitical X-ray energy dispersive spectroscopy. By using a combination of the different in situ microscopic techniques, the Antarctic microbial mats were found to be structures with vertical stratification of groups of cyanobacteria and mineral sediments, high contents of extracellular polymeric substances, and large void spaces occupied by water. In cyanobacterium-rich layers, heterocystous nostocalean and nonheterocystous oscillatorialean taxa were the most abundant taxa and appeared to be intermixed with fine-size deposits of epicellular silica and calcium carbonate. Most of the cyanobacterial filaments had similar orientations in zones without sediment particles, but thin filaments were tangled among thicker filaments. The combination of the microscopic techniques used showed the relative positions of biological and mineral entities within the microbial mats and enabled some speculation about their interactions. This work was supported by grants BOS2000-1121, REN2002-03542, ANT96-2174-E, ANT99-1319-E, and REN2000-0435-ANT. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Ríos, Asunción de los Ascaso, Carmen Wierzchos, Jacek Fernández-Valiente, Eduardo Quesada, Antonio |
spellingShingle |
Ríos, Asunción de los Ascaso, Carmen Wierzchos, Jacek Fernández-Valiente, Eduardo Quesada, Antonio Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica |
author_facet |
Ríos, Asunción de los Ascaso, Carmen Wierzchos, Jacek Fernández-Valiente, Eduardo Quesada, Antonio |
author_sort |
Ríos, Asunción de los |
title |
Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica |
title_short |
Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica |
title_full |
Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica |
title_fullStr |
Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica |
title_full_unstemmed |
Microstructural Characterization of Cyanobacterial Mats from the McMurdo Ice Shelf, Antarctica |
title_sort |
microstructural characterization of cyanobacterial mats from the mcmurdo ice shelf, antarctica |
publisher |
American Society for Microbiology |
publishDate |
2004 |
url |
http://hdl.handle.net/10261/2842 https://doi.org/10.1128/AEM.70.1.569-580.2004 |
long_lat |
ENVELOPE(166.500,166.500,-78.000,-78.000) |
geographic |
Antarctic McMurdo Ice Shelf The Antarctic |
geographic_facet |
Antarctic McMurdo Ice Shelf The Antarctic |
genre |
Antarc* Antarctic Antarctica Ice Shelf McMurdo Ice Shelf |
genre_facet |
Antarc* Antarctic Antarctica Ice Shelf McMurdo Ice Shelf |
op_relation |
Appl Environ Microbiol. 2004 January; 70(1): 569–580. 0099-2240 http://hdl.handle.net/10261/2842 doi:10.1128/AEM.70.1.569-580.2004 14711689 |
op_rights |
open |
op_doi |
https://doi.org/10.1128/AEM.70.1.569-580.2004 |
container_title |
Applied and Environmental Microbiology |
container_volume |
70 |
container_issue |
1 |
container_start_page |
569 |
op_container_end_page |
580 |
_version_ |
1790601041799020544 |