Sperm cryopreservation in brown bear (Ursus arctos): Preliminary aspects
The development of sperm cryopreservation procedures in brown bear is the basis for establishing a specific genetic resource bank aimed at the preservation of a Cantabric brown bear population, which is seriously threatened. Several issues complicate the development of these cryopreservation procedu...
Published in: | Reproduction in Domestic Animals |
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Blackwell Publishing
2008
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Online Access: | http://hdl.handle.net/10261/283896 https://doi.org/10.1111/j.1439-0531.2008.01248.x https://doi.org/10.13039/501100007273 https://doi.org/10.13039/501100004837 |
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ftcsic:oai:digital.csic.es:10261/283896 2024-02-11T10:09:20+01:00 Sperm cryopreservation in brown bear (Ursus arctos): Preliminary aspects Anel, Luis Álvarez, Mercedes Martínez-Pastor, Felipe Gomes-Alves, Susana Nicolás, M. Mata, Manuel Martínez, A. F. Borragán, Santiago Anel, Enrique Paz, Paulino de Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Universidad de León Ministerio de Ciencia e Innovación (España) 2008 application/pdf http://hdl.handle.net/10261/283896 https://doi.org/10.1111/j.1439-0531.2008.01248.x https://doi.org/10.13039/501100007273 https://doi.org/10.13039/501100004837 en eng Blackwell Publishing Preprint https://doi.org/10.1111/j.1439-0531.2008.01248.x Sí Reproduction in Domestic Animals 43(s4): 9-17 (2008) 0936-6768 http://hdl.handle.net/10261/283896 doi:10.1111/j.1439-0531.2008.01248.x http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100004837 18803752 open artículo 2008 ftcsic https://doi.org/10.1111/j.1439-0531.2008.01248.x10.13039/50110000727310.13039/501100004837 2024-01-16T11:31:29Z The development of sperm cryopreservation procedures in brown bear is the basis for establishing a specific genetic resource bank aimed at the preservation of a Cantabric brown bear population, which is seriously threatened. Several issues complicate the development of these cryopreservation procedures: lack of previous specific studies, a high incidence of urospermia and spermagglutination observed in bear ejaculates. Moreover, the availability of individuals for research from these threatened populations is problematic. In the case of the Cantabric brown bear, we have used males from other populations, but of the same species, as surrogates, to carry out a direct extrapolation of the results. Urospermia – Moreover, 70% of the ejaculates are urine contaminated and spermagglutination have a detrimental effect on post-thawing cell quality recovery in this species. Considering the high value of these samples (autochthonous population with few individuals), a pre-selection of the ejaculates is not a viable alternative. Preventive methods reducing the mentioned detrimental effects need to be developed. On the basis of previous data, we can suppose that bear spermatozoa resist freezing injuries well. Nevertheless, because of the scarcity of this information, it is necessary to conduct further research on bear semen freezing under field conditions. Epidydimal spermatozoa can be important for genetic resource banking of threatened populations and thus specific cryobiological protocols need to be assayed. To date, 168 brown bear ejaculates have been frozen by the ITRA-ULE group at the University of León (Spain) in the development of methodologies for the preservation of brown bear sperm. Our experiments have been supported in part by CICYT (CLG2004-02178/BOS, CLG2007-63748/BOS), CANTUR S.A. and University of Leon. Felipe Martínez-Pastor was supported by the Juan de la Cierva program (Spanish Ministry of Science and Innovation). Peer reviewed Article in Journal/Newspaper Ursus arctos Digital.CSIC (Spanish National Research Council) Cierva ENVELOPE(-60.873,-60.873,-64.156,-64.156) Martínez ENVELOPE(-62.183,-62.183,-64.650,-64.650) Reproduction in Domestic Animals 43 9 17 |
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Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
The development of sperm cryopreservation procedures in brown bear is the basis for establishing a specific genetic resource bank aimed at the preservation of a Cantabric brown bear population, which is seriously threatened. Several issues complicate the development of these cryopreservation procedures: lack of previous specific studies, a high incidence of urospermia and spermagglutination observed in bear ejaculates. Moreover, the availability of individuals for research from these threatened populations is problematic. In the case of the Cantabric brown bear, we have used males from other populations, but of the same species, as surrogates, to carry out a direct extrapolation of the results. Urospermia – Moreover, 70% of the ejaculates are urine contaminated and spermagglutination have a detrimental effect on post-thawing cell quality recovery in this species. Considering the high value of these samples (autochthonous population with few individuals), a pre-selection of the ejaculates is not a viable alternative. Preventive methods reducing the mentioned detrimental effects need to be developed. On the basis of previous data, we can suppose that bear spermatozoa resist freezing injuries well. Nevertheless, because of the scarcity of this information, it is necessary to conduct further research on bear semen freezing under field conditions. Epidydimal spermatozoa can be important for genetic resource banking of threatened populations and thus specific cryobiological protocols need to be assayed. To date, 168 brown bear ejaculates have been frozen by the ITRA-ULE group at the University of León (Spain) in the development of methodologies for the preservation of brown bear sperm. Our experiments have been supported in part by CICYT (CLG2004-02178/BOS, CLG2007-63748/BOS), CANTUR S.A. and University of Leon. Felipe Martínez-Pastor was supported by the Juan de la Cierva program (Spanish Ministry of Science and Innovation). Peer reviewed |
author2 |
Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Universidad de León Ministerio de Ciencia e Innovación (España) |
format |
Article in Journal/Newspaper |
author |
Anel, Luis Álvarez, Mercedes Martínez-Pastor, Felipe Gomes-Alves, Susana Nicolás, M. Mata, Manuel Martínez, A. F. Borragán, Santiago Anel, Enrique Paz, Paulino de |
spellingShingle |
Anel, Luis Álvarez, Mercedes Martínez-Pastor, Felipe Gomes-Alves, Susana Nicolás, M. Mata, Manuel Martínez, A. F. Borragán, Santiago Anel, Enrique Paz, Paulino de Sperm cryopreservation in brown bear (Ursus arctos): Preliminary aspects |
author_facet |
Anel, Luis Álvarez, Mercedes Martínez-Pastor, Felipe Gomes-Alves, Susana Nicolás, M. Mata, Manuel Martínez, A. F. Borragán, Santiago Anel, Enrique Paz, Paulino de |
author_sort |
Anel, Luis |
title |
Sperm cryopreservation in brown bear (Ursus arctos): Preliminary aspects |
title_short |
Sperm cryopreservation in brown bear (Ursus arctos): Preliminary aspects |
title_full |
Sperm cryopreservation in brown bear (Ursus arctos): Preliminary aspects |
title_fullStr |
Sperm cryopreservation in brown bear (Ursus arctos): Preliminary aspects |
title_full_unstemmed |
Sperm cryopreservation in brown bear (Ursus arctos): Preliminary aspects |
title_sort |
sperm cryopreservation in brown bear (ursus arctos): preliminary aspects |
publisher |
Blackwell Publishing |
publishDate |
2008 |
url |
http://hdl.handle.net/10261/283896 https://doi.org/10.1111/j.1439-0531.2008.01248.x https://doi.org/10.13039/501100007273 https://doi.org/10.13039/501100004837 |
long_lat |
ENVELOPE(-60.873,-60.873,-64.156,-64.156) ENVELOPE(-62.183,-62.183,-64.650,-64.650) |
geographic |
Cierva Martínez |
geographic_facet |
Cierva Martínez |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_relation |
Preprint https://doi.org/10.1111/j.1439-0531.2008.01248.x Sí Reproduction in Domestic Animals 43(s4): 9-17 (2008) 0936-6768 http://hdl.handle.net/10261/283896 doi:10.1111/j.1439-0531.2008.01248.x http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100004837 18803752 |
op_rights |
open |
op_doi |
https://doi.org/10.1111/j.1439-0531.2008.01248.x10.13039/50110000727310.13039/501100004837 |
container_title |
Reproduction in Domestic Animals |
container_volume |
43 |
container_start_page |
9 |
op_container_end_page |
17 |
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1790609192533360640 |