Neospora caninum antibodies in wild carnivores from Spain
Serum samples from 251 wild carnivores from different regions of Spain were tested for antibodies to Neospora caninum by the commercial competitive screening enzyme linked immunosorbent assay (c-ELISA) and confirmed by Neospora agglutination test (NAT) and/or by indirect fluorescent antibody test (I...
Published in: | Veterinary Parasitology |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Elsevier
2008
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Online Access: | http://hdl.handle.net/10261/283280 https://doi.org/10.1016/j.vetpar.2008.05.009 https://doi.org/10.13039/501100007273 https://doi.org/10.13039/501100011011 https://doi.org/10.13039/501100011698 https://doi.org/10.13039/100011941 |
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ftcsic:oai:digital.csic.es:10261/283280 2024-02-11T10:02:49+01:00 Neospora caninum antibodies in wild carnivores from Spain Sobrino, Raquel Dubey, Jitender P. Pabón, Marcela Linarez, N. Kwok, Oliver Chun Hung Millán, Javier Arnal, Maria Cruz Luco, Daniel F. López-Gatius, Fernando Thulliez, Philippe Gortázar, Christian Almería, Sonia Junta de Comunidades de Castilla-La Mancha Principado de Asturias Organismo Autónomo Parques Nacionales (España) Ministerio de Agricultura (España) Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Junta de Andalucía 2008 application/pdf http://hdl.handle.net/10261/283280 https://doi.org/10.1016/j.vetpar.2008.05.009 https://doi.org/10.13039/501100007273 https://doi.org/10.13039/501100011011 https://doi.org/10.13039/501100011698 https://doi.org/10.13039/100011941 en eng Elsevier #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MEC//AGL2007-65521-C02-02/ES/EFECTO DEL ESTRES CLIMATICO Y DE LA UTILIZACION DE TECNICAS PALIATIVAS SOBRE EL RIESGO DE ABORTO EN VACAS LECHERAS SEROPOSITIVAS A NEOSPORA CANINUM/ info:eu-repo/grantAgreement/MEC//AGL2007-65521-C02-01/ES/ASPECTOS ENDOCRINOS E INMUNOLOGICOS ASOCIADOS CON LA INFECCION Y EL ABORTO EN GANADO VACUNO Y ESTUDIO DEL PAPEL DE LOS CARNIVOROS SILVESTRES EN EL CICLO SILVATICO DEL PARASITO/ https://doi.org/10.1016/j.vetpar.2008.05.009 Sí Veterinary Parasitology 155(3-4): 190-197 (2008) 0304-4017 http://hdl.handle.net/10261/283280 doi:10.1016/j.vetpar.2008.05.009 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100011698 http://dx.doi.org/10.13039/100011941 18556128 none artículo 2008 ftcsic https://doi.org/10.1016/j.vetpar.2008.05.00910.13039/50110000727310.13039/50110001101110.13039/50110001169810.13039/100011941 2024-01-16T11:31:08Z Serum samples from 251 wild carnivores from different regions of Spain were tested for antibodies to Neospora caninum by the commercial competitive screening enzyme linked immunosorbent assay (c-ELISA) and confirmed by Neospora agglutination test (NAT) and/or by indirect fluorescent antibody test (IFAT). Samples with antibodies detected by at least two serological tests were considered seropositive. Antibodies to N. caninum were found in 3.2% of 95 red foxes (Vulpes vulpes); in 21.4% of 28 wolves (Canis lupus); in 12.0% of 25 Iberian lynx (Lynx pardinus); in 16.7% of 6 European wildcats (Felis silvestris); in 6.4% of 31 Eurasian badgers (Meles meles); in 21.4% of 14 stone martens (Martes foina); in 66.7% of 3 pine martens (M. martes) and in 50% of 2 polecats (Mustela putorius). Antibodies to N. caninum in common genets (Genetta genetta) and Egyptian mongooses (Herpestes ichneumon) were only observed by c-ELISA but were not confirmed by IFAT and/or NAT. No antibodies were detected in 5 Eurasian otters (Lutra lutra) by any technique. Statistically significant differences were observed among species and among geographical areas. The highest seroprevalence of N. caninum infection was observed in the Cantabric Coastal region characterized by high humidity. To our knowledge, this is the first report of antibodies to N. caninum in free ranging wild carnivores, other than wild canids, in Europe. The existence of a possible sylvatic cycle could have important implications in both sylvatic and domestic cycles since they might influence the prevalence of infection in cattle farms in those areas. This study is a contribution to GC-05-006, ICS, JCCM, and agreements between IREC and Principado de Asturias and Ministerio de Agricultura and OAPN, Spain. This study received partially support from the Spanish CICYT, grants AGL2004-06103, AGL2005-07401 and AGL2007-65521. This study was also partially included in the “Programa de Actuaciones para la Conservación del Lince en Andalucía II”. Consejería del Medio Ambiente, Junta de ... Article in Journal/Newspaper Canis lupus Lince Lutra lutra Lynx Digital.CSIC (Spanish National Research Council) Veterinary Parasitology 155 3-4 190 197 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
Serum samples from 251 wild carnivores from different regions of Spain were tested for antibodies to Neospora caninum by the commercial competitive screening enzyme linked immunosorbent assay (c-ELISA) and confirmed by Neospora agglutination test (NAT) and/or by indirect fluorescent antibody test (IFAT). Samples with antibodies detected by at least two serological tests were considered seropositive. Antibodies to N. caninum were found in 3.2% of 95 red foxes (Vulpes vulpes); in 21.4% of 28 wolves (Canis lupus); in 12.0% of 25 Iberian lynx (Lynx pardinus); in 16.7% of 6 European wildcats (Felis silvestris); in 6.4% of 31 Eurasian badgers (Meles meles); in 21.4% of 14 stone martens (Martes foina); in 66.7% of 3 pine martens (M. martes) and in 50% of 2 polecats (Mustela putorius). Antibodies to N. caninum in common genets (Genetta genetta) and Egyptian mongooses (Herpestes ichneumon) were only observed by c-ELISA but were not confirmed by IFAT and/or NAT. No antibodies were detected in 5 Eurasian otters (Lutra lutra) by any technique. Statistically significant differences were observed among species and among geographical areas. The highest seroprevalence of N. caninum infection was observed in the Cantabric Coastal region characterized by high humidity. To our knowledge, this is the first report of antibodies to N. caninum in free ranging wild carnivores, other than wild canids, in Europe. The existence of a possible sylvatic cycle could have important implications in both sylvatic and domestic cycles since they might influence the prevalence of infection in cattle farms in those areas. This study is a contribution to GC-05-006, ICS, JCCM, and agreements between IREC and Principado de Asturias and Ministerio de Agricultura and OAPN, Spain. This study received partially support from the Spanish CICYT, grants AGL2004-06103, AGL2005-07401 and AGL2007-65521. This study was also partially included in the “Programa de Actuaciones para la Conservación del Lince en Andalucía II”. Consejería del Medio Ambiente, Junta de ... |
author2 |
Junta de Comunidades de Castilla-La Mancha Principado de Asturias Organismo Autónomo Parques Nacionales (España) Ministerio de Agricultura (España) Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Junta de Andalucía |
format |
Article in Journal/Newspaper |
author |
Sobrino, Raquel Dubey, Jitender P. Pabón, Marcela Linarez, N. Kwok, Oliver Chun Hung Millán, Javier Arnal, Maria Cruz Luco, Daniel F. López-Gatius, Fernando Thulliez, Philippe Gortázar, Christian Almería, Sonia |
spellingShingle |
Sobrino, Raquel Dubey, Jitender P. Pabón, Marcela Linarez, N. Kwok, Oliver Chun Hung Millán, Javier Arnal, Maria Cruz Luco, Daniel F. López-Gatius, Fernando Thulliez, Philippe Gortázar, Christian Almería, Sonia Neospora caninum antibodies in wild carnivores from Spain |
author_facet |
Sobrino, Raquel Dubey, Jitender P. Pabón, Marcela Linarez, N. Kwok, Oliver Chun Hung Millán, Javier Arnal, Maria Cruz Luco, Daniel F. López-Gatius, Fernando Thulliez, Philippe Gortázar, Christian Almería, Sonia |
author_sort |
Sobrino, Raquel |
title |
Neospora caninum antibodies in wild carnivores from Spain |
title_short |
Neospora caninum antibodies in wild carnivores from Spain |
title_full |
Neospora caninum antibodies in wild carnivores from Spain |
title_fullStr |
Neospora caninum antibodies in wild carnivores from Spain |
title_full_unstemmed |
Neospora caninum antibodies in wild carnivores from Spain |
title_sort |
neospora caninum antibodies in wild carnivores from spain |
publisher |
Elsevier |
publishDate |
2008 |
url |
http://hdl.handle.net/10261/283280 https://doi.org/10.1016/j.vetpar.2008.05.009 https://doi.org/10.13039/501100007273 https://doi.org/10.13039/501100011011 https://doi.org/10.13039/501100011698 https://doi.org/10.13039/100011941 |
genre |
Canis lupus Lince Lutra lutra Lynx |
genre_facet |
Canis lupus Lince Lutra lutra Lynx |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MEC//AGL2007-65521-C02-02/ES/EFECTO DEL ESTRES CLIMATICO Y DE LA UTILIZACION DE TECNICAS PALIATIVAS SOBRE EL RIESGO DE ABORTO EN VACAS LECHERAS SEROPOSITIVAS A NEOSPORA CANINUM/ info:eu-repo/grantAgreement/MEC//AGL2007-65521-C02-01/ES/ASPECTOS ENDOCRINOS E INMUNOLOGICOS ASOCIADOS CON LA INFECCION Y EL ABORTO EN GANADO VACUNO Y ESTUDIO DEL PAPEL DE LOS CARNIVOROS SILVESTRES EN EL CICLO SILVATICO DEL PARASITO/ https://doi.org/10.1016/j.vetpar.2008.05.009 Sí Veterinary Parasitology 155(3-4): 190-197 (2008) 0304-4017 http://hdl.handle.net/10261/283280 doi:10.1016/j.vetpar.2008.05.009 http://dx.doi.org/10.13039/501100007273 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100011698 http://dx.doi.org/10.13039/100011941 18556128 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.vetpar.2008.05.00910.13039/50110000727310.13039/50110001101110.13039/50110001169810.13039/100011941 |
container_title |
Veterinary Parasitology |
container_volume |
155 |
container_issue |
3-4 |
container_start_page |
190 |
op_container_end_page |
197 |
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1790598887384285184 |