Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes
12 pages, 4 figures, 2 tables,1 appendix supplementary materials https://doi.org/10.1016/j.hal.2022.102292.-- Data availability: Data will be made available on request Phaeocystis is a globally widespread marine phytoplankton genus, best known for its colony-forming species that can form large bloom...
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ftcsic:oai:digital.csic.es:10261/278479 2024-02-11T09:55:50+01:00 Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes Koppelle, Sebastiaan López-Escardó, David Brussaard, Corina P.D. Huisman, Jef Philippart, Catharina J.M. Massana, Ramon Wilken, Susanne Agencia Estatal de Investigación (España) 2022-08 http://hdl.handle.net/10261/278479 https://doi.org/10.1016/j.hal.2022.102292 https://doi.org/10.13039/501100011033 en eng Elsevier Publisher's version https://doi.org/10.1016/j.hal.2022.102292 Sí Harmful Algae 117: 102292 (2022) 1568-9883 CEX2019-000928-S http://hdl.handle.net/10261/278479 doi:10.1016/j.hal.2022.102292 1878-1470 http://dx.doi.org/10.13039/501100011033 open Phaeocystis globosa Mixotrophy Bacterivory Confocal microscopy Haptophyte artículo 2022 ftcsic https://doi.org/10.1016/j.hal.2022.10229210.13039/501100011033 2024-01-16T11:28:34Z 12 pages, 4 figures, 2 tables,1 appendix supplementary materials https://doi.org/10.1016/j.hal.2022.102292.-- Data availability: Data will be made available on request Phaeocystis is a globally widespread marine phytoplankton genus, best known for its colony-forming species that can form large blooms and odorous foam during bloom decline. In the North Sea, Phaeocystis globosa typically becomes abundant towards the end of the spring bloom, when nutrients are depleted and the share of mixotrophic protists increases. Although mixotrophy is widespread across the eukaryotic tree of life and is also found amongst haptophytes, a mixotrophic nutrition has not yet been demonstrated in Phaeocystis. Here, we sampled two consecutive Phaeocystis globosa spring blooms in the coastal North Sea. In both years, bacterial cells were observed inside 0.6 – 2% of P. globosa cells using double CARD-FISH hybridizations in combination with laser scanning confocal microscopy. Incubation experiments manipulating light and nutrient availability showed a trend towards higher occurrence of intracellular bacteria under P-deplete conditions. Based on counts of bacteria inside P. globosa cells in combination with theoretical values of prey digestion times, maximum ingestion rates of up to 0.08 bacteria cell−1 h−1 were estimated. In addition, a gene-based predictive model was applied to the transcriptome assemblies of seven Phaeocystis strains and 24 other haptophytes to assess their trophic mode. This model predicted a phago-mixotrophic feeding strategy in several (but not all) strains of P. globosa, P. antarctica and other haptophytes that were previously assumed to be autotrophic. The observation of bacterial cells inside P. globosa and the gene-based model predictions strongly suggest that the phago-mixotrophic feeding strategy is widespread among members of the Phaeocystis genus and other haptophytes, and might contribute to their remarkable success to form nuisance blooms under nutrient-limiting conditions This work was supported by a ... Article in Journal/Newspaper Antarc* Antarctica Digital.CSIC (Spanish National Research Council) Harmful Algae 117 102292 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
language |
English |
topic |
Phaeocystis globosa Mixotrophy Bacterivory Confocal microscopy Haptophyte |
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Phaeocystis globosa Mixotrophy Bacterivory Confocal microscopy Haptophyte Koppelle, Sebastiaan López-Escardó, David Brussaard, Corina P.D. Huisman, Jef Philippart, Catharina J.M. Massana, Ramon Wilken, Susanne Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
topic_facet |
Phaeocystis globosa Mixotrophy Bacterivory Confocal microscopy Haptophyte |
description |
12 pages, 4 figures, 2 tables,1 appendix supplementary materials https://doi.org/10.1016/j.hal.2022.102292.-- Data availability: Data will be made available on request Phaeocystis is a globally widespread marine phytoplankton genus, best known for its colony-forming species that can form large blooms and odorous foam during bloom decline. In the North Sea, Phaeocystis globosa typically becomes abundant towards the end of the spring bloom, when nutrients are depleted and the share of mixotrophic protists increases. Although mixotrophy is widespread across the eukaryotic tree of life and is also found amongst haptophytes, a mixotrophic nutrition has not yet been demonstrated in Phaeocystis. Here, we sampled two consecutive Phaeocystis globosa spring blooms in the coastal North Sea. In both years, bacterial cells were observed inside 0.6 – 2% of P. globosa cells using double CARD-FISH hybridizations in combination with laser scanning confocal microscopy. Incubation experiments manipulating light and nutrient availability showed a trend towards higher occurrence of intracellular bacteria under P-deplete conditions. Based on counts of bacteria inside P. globosa cells in combination with theoretical values of prey digestion times, maximum ingestion rates of up to 0.08 bacteria cell−1 h−1 were estimated. In addition, a gene-based predictive model was applied to the transcriptome assemblies of seven Phaeocystis strains and 24 other haptophytes to assess their trophic mode. This model predicted a phago-mixotrophic feeding strategy in several (but not all) strains of P. globosa, P. antarctica and other haptophytes that were previously assumed to be autotrophic. The observation of bacterial cells inside P. globosa and the gene-based model predictions strongly suggest that the phago-mixotrophic feeding strategy is widespread among members of the Phaeocystis genus and other haptophytes, and might contribute to their remarkable success to form nuisance blooms under nutrient-limiting conditions This work was supported by a ... |
author2 |
Agencia Estatal de Investigación (España) |
format |
Article in Journal/Newspaper |
author |
Koppelle, Sebastiaan López-Escardó, David Brussaard, Corina P.D. Huisman, Jef Philippart, Catharina J.M. Massana, Ramon Wilken, Susanne |
author_facet |
Koppelle, Sebastiaan López-Escardó, David Brussaard, Corina P.D. Huisman, Jef Philippart, Catharina J.M. Massana, Ramon Wilken, Susanne |
author_sort |
Koppelle, Sebastiaan |
title |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_short |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_full |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_fullStr |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_full_unstemmed |
Mixotrophy in the bloom-forming genus Phaeocystis and other haptophytes |
title_sort |
mixotrophy in the bloom-forming genus phaeocystis and other haptophytes |
publisher |
Elsevier |
publishDate |
2022 |
url |
http://hdl.handle.net/10261/278479 https://doi.org/10.1016/j.hal.2022.102292 https://doi.org/10.13039/501100011033 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
Publisher's version https://doi.org/10.1016/j.hal.2022.102292 Sí Harmful Algae 117: 102292 (2022) 1568-9883 CEX2019-000928-S http://hdl.handle.net/10261/278479 doi:10.1016/j.hal.2022.102292 1878-1470 http://dx.doi.org/10.13039/501100011033 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.hal.2022.10229210.13039/501100011033 |
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Harmful Algae |
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117 |
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102292 |
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