Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem
14 pages, 3 figures, 5 tables Spatial patterns in prokaryotic biodiversity and production were assessed in the Mackenzie shelf region of the Beaufort Sea during open-water conditions. The sampling transect extended 350 km northwards, from upstream freshwater sites in the Mackenzie River to coastal a...
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ftcsic:oai:digital.csic.es:10261/27392 2024-02-11T10:00:43+01:00 Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem Garneau, Marie-Ève Vicent, Warwick F. Alonso-Sáez, Laura Gratton, Yves Lovejoy, Connie 2006-02-06 5867 bytes application/pdf http://hdl.handle.net/10261/27392 https://doi.org/10.3354/ame042027 en eng Inter Research https://doi.org/10.3354/ame042027 Aquatic Microbial Ecology 42(1): 27-40 (2006) 0948-3055 http://hdl.handle.net/10261/27392 doi:10.3354/ame042027 1616-1564 open Prokaryote diversity Archaea Proteobacteria Cytophaga-Flavobacter-Bacteroides Arctic Ocean Mackenzie River estuary Picocyanobacteria CARD-FISH artículo http://purl.org/coar/resource_type/c_6501 2006 ftcsic https://doi.org/10.3354/ame042027 2024-01-16T09:28:11Z 14 pages, 3 figures, 5 tables Spatial patterns in prokaryotic biodiversity and production were assessed in the Mackenzie shelf region of the Beaufort Sea during open-water conditions. The sampling transect extended 350 km northwards, from upstream freshwater sites in the Mackenzie River to coastal and offshore sites, towards the edge of the perennial arctic ice pack. The analyses revealed strong gradients in community structure and prokaryotic cell concentrations, both of which correlated with salinity. Picocyanobacterial abundance was low (102 to 103 cells ml–1), particularly at the offshore stations that were least influenced by the river plume. Analysis by catalyzed reporter deposition for fluorescence in situ hybridization (CARD-FISH) showed that the dominant heterotrophic cell types were β-Proteobacteria at river sites, shifting to dominance by α-Proteobacteria offshore. Cells in the Cytophaga–Flavobacter–Bacteroides and γ-Proteobacteria groups each contributed <5% of total counts in the river, but >10% of counts in the marine samples. Archaea were detected among the surface-water microbiota, contributing on average 1.3% of the total DAPI counts in marine samples, but 6.0% in turbid coastal and riverine waters. 3H-leucine uptake rates were significantly higher at 2 stations influenced by the river (1.5 pmol l–1 h–1) than at other marine stations or in the river itself (≤0.5 pmol–1 h–1). Size-fractionation experiments at 2 coastal sites showed that >65% of heterotrophic production was associated with particles >3 µm. These results indicate the importance of particle-attached prokaryotes, and imply a broad functional diversity of heterotrophic microbes that likely facilitates breakdown of the heterogeneous dissolved and particulate terrestrial materials discharged into arctic seas This study was made possible with financial support from the Natural Sciences and Engineering Research Council of Canada (NSERC), the Canada Research Chair program, Fonds québécois de recherche sur la nature et les ... Article in Journal/Newspaper Arctic Arctic Ocean Beaufort Sea ice pack Mackenzie river Mackenzie Shelf Digital.CSIC (Spanish National Research Council) Arctic Arctic Ocean Canada Mackenzie River Aquatic Microbial Ecology 42 27 40 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Prokaryote diversity Archaea Proteobacteria Cytophaga-Flavobacter-Bacteroides Arctic Ocean Mackenzie River estuary Picocyanobacteria CARD-FISH |
spellingShingle |
Prokaryote diversity Archaea Proteobacteria Cytophaga-Flavobacter-Bacteroides Arctic Ocean Mackenzie River estuary Picocyanobacteria CARD-FISH Garneau, Marie-Ève Vicent, Warwick F. Alonso-Sáez, Laura Gratton, Yves Lovejoy, Connie Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem |
topic_facet |
Prokaryote diversity Archaea Proteobacteria Cytophaga-Flavobacter-Bacteroides Arctic Ocean Mackenzie River estuary Picocyanobacteria CARD-FISH |
description |
14 pages, 3 figures, 5 tables Spatial patterns in prokaryotic biodiversity and production were assessed in the Mackenzie shelf region of the Beaufort Sea during open-water conditions. The sampling transect extended 350 km northwards, from upstream freshwater sites in the Mackenzie River to coastal and offshore sites, towards the edge of the perennial arctic ice pack. The analyses revealed strong gradients in community structure and prokaryotic cell concentrations, both of which correlated with salinity. Picocyanobacterial abundance was low (102 to 103 cells ml–1), particularly at the offshore stations that were least influenced by the river plume. Analysis by catalyzed reporter deposition for fluorescence in situ hybridization (CARD-FISH) showed that the dominant heterotrophic cell types were β-Proteobacteria at river sites, shifting to dominance by α-Proteobacteria offshore. Cells in the Cytophaga–Flavobacter–Bacteroides and γ-Proteobacteria groups each contributed <5% of total counts in the river, but >10% of counts in the marine samples. Archaea were detected among the surface-water microbiota, contributing on average 1.3% of the total DAPI counts in marine samples, but 6.0% in turbid coastal and riverine waters. 3H-leucine uptake rates were significantly higher at 2 stations influenced by the river (1.5 pmol l–1 h–1) than at other marine stations or in the river itself (≤0.5 pmol–1 h–1). Size-fractionation experiments at 2 coastal sites showed that >65% of heterotrophic production was associated with particles >3 µm. These results indicate the importance of particle-attached prokaryotes, and imply a broad functional diversity of heterotrophic microbes that likely facilitates breakdown of the heterogeneous dissolved and particulate terrestrial materials discharged into arctic seas This study was made possible with financial support from the Natural Sciences and Engineering Research Council of Canada (NSERC), the Canada Research Chair program, Fonds québécois de recherche sur la nature et les ... |
format |
Article in Journal/Newspaper |
author |
Garneau, Marie-Ève Vicent, Warwick F. Alonso-Sáez, Laura Gratton, Yves Lovejoy, Connie |
author_facet |
Garneau, Marie-Ève Vicent, Warwick F. Alonso-Sáez, Laura Gratton, Yves Lovejoy, Connie |
author_sort |
Garneau, Marie-Ève |
title |
Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem |
title_short |
Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem |
title_full |
Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem |
title_fullStr |
Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem |
title_full_unstemmed |
Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem |
title_sort |
prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem |
publisher |
Inter Research |
publishDate |
2006 |
url |
http://hdl.handle.net/10261/27392 https://doi.org/10.3354/ame042027 |
geographic |
Arctic Arctic Ocean Canada Mackenzie River |
geographic_facet |
Arctic Arctic Ocean Canada Mackenzie River |
genre |
Arctic Arctic Ocean Beaufort Sea ice pack Mackenzie river Mackenzie Shelf |
genre_facet |
Arctic Arctic Ocean Beaufort Sea ice pack Mackenzie river Mackenzie Shelf |
op_relation |
https://doi.org/10.3354/ame042027 Aquatic Microbial Ecology 42(1): 27-40 (2006) 0948-3055 http://hdl.handle.net/10261/27392 doi:10.3354/ame042027 1616-1564 |
op_rights |
open |
op_doi |
https://doi.org/10.3354/ame042027 |
container_title |
Aquatic Microbial Ecology |
container_volume |
42 |
container_start_page |
27 |
op_container_end_page |
40 |
_version_ |
1790596433468981248 |