Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean
11 pages, 4 figures, 3 tables Bacterioplankton have the potential to significantly affect the cycling of organic matter in the ocean; however, little is known about the linkage between bacterial assemblage structure and carbon metabolism. In this study, we investigated whether changes in the phyloge...
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ftcsic:oai:digital.csic.es:10261/26797 2024-02-11T10:06:38+01:00 Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean Alonso-Sáez, Laura Arístegui, Javier Pinhassi, Jarone Gómez-Consarnau, Laura González, José M. Vaqué, Dolors Agustí, Susana Gasol, Josep M. 2007-01-19 5867 bytes application/pdf http://hdl.handle.net/10261/26797 https://doi.org/10.3354/ame046043 en eng Inter Research https://doi.org/10.3354/ame046043 Aquatic Microbial Ecology 46(1): 43-53 (2007) 0948-3055 http://hdl.handle.net/10261/26797 doi:10.3354/ame046043 open Bacteria Atlantic Ocean Diversity Metabolism DGGE CARD-FISH artículo http://purl.org/coar/resource_type/c_6501 2007 ftcsic https://doi.org/10.3354/ame046043 2024-01-16T09:27:54Z 11 pages, 4 figures, 3 tables Bacterioplankton have the potential to significantly affect the cycling of organic matter in the ocean; however, little is known about the linkage between bacterial assemblage structure and carbon metabolism. In this study, we investigated whether changes in the phylogenetic composition of bacterioplankton were associated with changes in bacterial carbon processing (bacterial production, respiration and biomass) in the subtropical NE Atlantic Ocean. We found consistent differences in the composition of the bacterial assemblage, as revealed by denaturing gradient gel electrophoresis (DGGE) and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH), along a gradient from the NW African upwelling to the oligotrophic North Atlantic Subtropical Gyre. The percent contribution of Bacteroidetes, Roseobacter and Gammaproteobacteria significantly increased towards more productive waters, whereas the SAR11 clade of the Alphaproteobacteria remained relatively constant (average 28% of DAPI-stained cells) throughout the area. Changes in the composition of the bacterial assemblage detected by DGGE were weakly but significantly correlated with changes in carbon processing variables. The abundances of Roseobacter and Gammaproteobacteria were highly correlated with the concentration of particulate organic carbon and chlorophyll a, reflecting the affinity of these groups to nutrient-enriched conditions. The abundance of Roseobacter was also positively correlated with heterotrophic bacterial production, suggesting their active participation in carbon processing Fieldwork was supported by the COCA (REN2000 1471-CO2-O1-MAR) and DEBACOCA (REN2001-4211-E) projects. Molecular work was funded by projects MICRODIFF (REN2001-2120/MAR), BASICS (EVK3-CT- 2002-00078) and GENMUMAR (CTM2004-02586/MAR). This is a contribution to the MARBEF European Network of Excellence (FP6-2002-Global-1). Financial support was provided by a Ph D fellowship from the Spanish government to L.A.S. Peer ... Article in Journal/Newspaper North Atlantic Digital.CSIC (Spanish National Research Council) Aquatic Microbial Ecology 46 43 53 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Bacteria Atlantic Ocean Diversity Metabolism DGGE CARD-FISH |
spellingShingle |
Bacteria Atlantic Ocean Diversity Metabolism DGGE CARD-FISH Alonso-Sáez, Laura Arístegui, Javier Pinhassi, Jarone Gómez-Consarnau, Laura González, José M. Vaqué, Dolors Agustí, Susana Gasol, Josep M. Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean |
topic_facet |
Bacteria Atlantic Ocean Diversity Metabolism DGGE CARD-FISH |
description |
11 pages, 4 figures, 3 tables Bacterioplankton have the potential to significantly affect the cycling of organic matter in the ocean; however, little is known about the linkage between bacterial assemblage structure and carbon metabolism. In this study, we investigated whether changes in the phylogenetic composition of bacterioplankton were associated with changes in bacterial carbon processing (bacterial production, respiration and biomass) in the subtropical NE Atlantic Ocean. We found consistent differences in the composition of the bacterial assemblage, as revealed by denaturing gradient gel electrophoresis (DGGE) and catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH), along a gradient from the NW African upwelling to the oligotrophic North Atlantic Subtropical Gyre. The percent contribution of Bacteroidetes, Roseobacter and Gammaproteobacteria significantly increased towards more productive waters, whereas the SAR11 clade of the Alphaproteobacteria remained relatively constant (average 28% of DAPI-stained cells) throughout the area. Changes in the composition of the bacterial assemblage detected by DGGE were weakly but significantly correlated with changes in carbon processing variables. The abundances of Roseobacter and Gammaproteobacteria were highly correlated with the concentration of particulate organic carbon and chlorophyll a, reflecting the affinity of these groups to nutrient-enriched conditions. The abundance of Roseobacter was also positively correlated with heterotrophic bacterial production, suggesting their active participation in carbon processing Fieldwork was supported by the COCA (REN2000 1471-CO2-O1-MAR) and DEBACOCA (REN2001-4211-E) projects. Molecular work was funded by projects MICRODIFF (REN2001-2120/MAR), BASICS (EVK3-CT- 2002-00078) and GENMUMAR (CTM2004-02586/MAR). This is a contribution to the MARBEF European Network of Excellence (FP6-2002-Global-1). Financial support was provided by a Ph D fellowship from the Spanish government to L.A.S. Peer ... |
format |
Article in Journal/Newspaper |
author |
Alonso-Sáez, Laura Arístegui, Javier Pinhassi, Jarone Gómez-Consarnau, Laura González, José M. Vaqué, Dolors Agustí, Susana Gasol, Josep M. |
author_facet |
Alonso-Sáez, Laura Arístegui, Javier Pinhassi, Jarone Gómez-Consarnau, Laura González, José M. Vaqué, Dolors Agustí, Susana Gasol, Josep M. |
author_sort |
Alonso-Sáez, Laura |
title |
Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean |
title_short |
Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean |
title_full |
Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean |
title_fullStr |
Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean |
title_full_unstemmed |
Bacterial assemblage structure and carbon metabolism along a productivity gradient in the NE Atlantic Ocean |
title_sort |
bacterial assemblage structure and carbon metabolism along a productivity gradient in the ne atlantic ocean |
publisher |
Inter Research |
publishDate |
2007 |
url |
http://hdl.handle.net/10261/26797 https://doi.org/10.3354/ame046043 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
https://doi.org/10.3354/ame046043 Aquatic Microbial Ecology 46(1): 43-53 (2007) 0948-3055 http://hdl.handle.net/10261/26797 doi:10.3354/ame046043 |
op_rights |
open |
op_doi |
https://doi.org/10.3354/ame046043 |
container_title |
Aquatic Microbial Ecology |
container_volume |
46 |
container_start_page |
43 |
op_container_end_page |
53 |
_version_ |
1790604456167997440 |