Lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides
Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact on pro...
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ftcsic:oai:digital.csic.es:10261/267126 2024-02-11T10:02:31+01:00 Lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides Bautista-Expósito, Sara Vandenberg, Albert Peñas, Elena Frías, Juana Martínez Villaluenga, Cristina Natural Sciences and Engineering Research Council of Canada 2021 http://hdl.handle.net/10261/267126 https://doi.org/10.3389/fpls.2021.754287 https://doi.org/10.13039/501100000038 en eng Frontiers Media Publisher's version https://doi.org/10.3389/fpls.2021.754287 Sí Frontiers in Plant Science 12: 754287 (2021) http://hdl.handle.net/10261/267126 doi:10.3389/fpls.2021.754287 1664-462X http://dx.doi.org/10.13039/501100000038 open artículo http://purl.org/coar/resource_type/c_6501 2021 ftcsic https://doi.org/10.3389/fpls.2021.75428710.13039/501100000038 2024-01-16T11:23:07Z Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact on protein digestibility and bioactivity. The objective was to compare the effect of seed germination on protease activity, structure, and proteolysis of four selected legumes with contrasting seed coat polyphenol profiles (gray zero-tannin lentil [GZL], beluga lentil [BL], and dehulled red lentil [DL]; and zero tannin/low vicine–convicine fava bean [ZF]). Protein hydrolysis was characterized during germination and digestion with respect to proteins, peptides, and free amino acids (FAAs). In vitro antihypertensive and antioxidant activities of digests were investigated, and the peptidomic characterization [high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS)] and identification of bioactive fragments in intestinal digests were performed. Regardless of the seed type, germination increased protease activity and reduced the levels of phytic acid, trypsin inhibitors, and tannins (only in BL). A significant proteolysis of the 7S and 11S globulins and a concomitant increase of peptides and FAAs were observed in all sprouted legumes. Digestion kinetics in sprouts revealed a faster generation of FAAs and peptides than in dry seeds, with changes being more evident for DL, associated with a faster imbibition, germination, and sprout growth. In contrast, BL sprouts showed the lowest protein digestibility, likely due to a lower protease activity, seed structure disintegration, and higher anti-nutrient levels in comparison to GZL, DL, and ZF. Moreover, the digestion of sprouts resulted in a higher number of resistant peptides in DL and ZF that matched with previously reported bioactive sequences, suggesting a promising health potential of legume sprouts that was confirmed in vitro. The results ... Article in Journal/Newspaper Beluga Beluga* Digital.CSIC (Spanish National Research Council) Frontiers in Plant Science 12 |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
language |
English |
description |
Germination offers advantages to improve legume protein digestibility as it disintegrates seed structure and hydrolyzes proteins and anti-nutrients. Seed permeability (related to polyphenol content of seed coats) is an important factor affecting the duration of seed germination and its impact on protein digestibility and bioactivity. The objective was to compare the effect of seed germination on protease activity, structure, and proteolysis of four selected legumes with contrasting seed coat polyphenol profiles (gray zero-tannin lentil [GZL], beluga lentil [BL], and dehulled red lentil [DL]; and zero tannin/low vicine–convicine fava bean [ZF]). Protein hydrolysis was characterized during germination and digestion with respect to proteins, peptides, and free amino acids (FAAs). In vitro antihypertensive and antioxidant activities of digests were investigated, and the peptidomic characterization [high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS)] and identification of bioactive fragments in intestinal digests were performed. Regardless of the seed type, germination increased protease activity and reduced the levels of phytic acid, trypsin inhibitors, and tannins (only in BL). A significant proteolysis of the 7S and 11S globulins and a concomitant increase of peptides and FAAs were observed in all sprouted legumes. Digestion kinetics in sprouts revealed a faster generation of FAAs and peptides than in dry seeds, with changes being more evident for DL, associated with a faster imbibition, germination, and sprout growth. In contrast, BL sprouts showed the lowest protein digestibility, likely due to a lower protease activity, seed structure disintegration, and higher anti-nutrient levels in comparison to GZL, DL, and ZF. Moreover, the digestion of sprouts resulted in a higher number of resistant peptides in DL and ZF that matched with previously reported bioactive sequences, suggesting a promising health potential of legume sprouts that was confirmed in vitro. The results ... |
author2 |
Natural Sciences and Engineering Research Council of Canada |
format |
Article in Journal/Newspaper |
author |
Bautista-Expósito, Sara Vandenberg, Albert Peñas, Elena Frías, Juana Martínez Villaluenga, Cristina |
spellingShingle |
Bautista-Expósito, Sara Vandenberg, Albert Peñas, Elena Frías, Juana Martínez Villaluenga, Cristina Lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides |
author_facet |
Bautista-Expósito, Sara Vandenberg, Albert Peñas, Elena Frías, Juana Martínez Villaluenga, Cristina |
author_sort |
Bautista-Expósito, Sara |
title |
Lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides |
title_short |
Lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides |
title_full |
Lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides |
title_fullStr |
Lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides |
title_full_unstemmed |
Lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides |
title_sort |
lentil and fava bean with contrasting germination kinetics: a focus on digestion of proteins and bioactivity of resistant peptides |
publisher |
Frontiers Media |
publishDate |
2021 |
url |
http://hdl.handle.net/10261/267126 https://doi.org/10.3389/fpls.2021.754287 https://doi.org/10.13039/501100000038 |
genre |
Beluga Beluga* |
genre_facet |
Beluga Beluga* |
op_relation |
Publisher's version https://doi.org/10.3389/fpls.2021.754287 Sí Frontiers in Plant Science 12: 754287 (2021) http://hdl.handle.net/10261/267126 doi:10.3389/fpls.2021.754287 1664-462X http://dx.doi.org/10.13039/501100000038 |
op_rights |
open |
op_doi |
https://doi.org/10.3389/fpls.2021.75428710.13039/501100000038 |
container_title |
Frontiers in Plant Science |
container_volume |
12 |
_version_ |
1790598532837670912 |