Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production
7 pages, 4 figures. The effect of viral hemorrhagic septicemia virus (VHSV) in vitro infection on the nitric oxide (NO) production by turbot Scophthalmus maximus kidney macrophages has been addressed in the past. Previously, we had determined that only a small fraction of turbot possess head kidney...
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ftcsic:oai:digital.csic.es:10261/26266 2024-02-11T10:08:26+01:00 Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production Tafalla, Carolina Figueras Huerta, Antonio Novoa, Beatriz 2001-10 150957 bytes application/pdf http://hdl.handle.net/10261/26266 https://doi.org/10.3354/dao047101 en eng Inter Research http://dx.doi.org/10.3354/dao047101 Diseases of Aquatic Organisms 47(2): 101-107 (2001) 0177-5103 http://hdl.handle.net/10261/26266 doi:10.3354/dao047101 open Nitric oxide Lipopolysacharide Macrophage Viral hemorrhagic virus Turbot Scophthalmus maximus Macrophage activating factor Tumor necrosis factor α artículo http://purl.org/coar/resource_type/c_6501 2001 ftcsic https://doi.org/10.3354/dao047101 2024-01-16T09:27:40Z 7 pages, 4 figures. The effect of viral hemorrhagic septicemia virus (VHSV) in vitro infection on the nitric oxide (NO) production by turbot Scophthalmus maximus kidney macrophages has been addressed in the past. Previously, we had determined that only a small fraction of turbot possess head kidney macrophages that respond to a single exposure of lipopolysaccharide (LPS) with NO production (LPS-responsive macrophages), whereas macrophage cultures from other individuals were not activated by LPS alone and needed a combination of stimuli to respond (LPS-non-responsive macrophages). In the current work, we examined the effect of VHSV on NO production by macrophages characterized as LPS-responsive macrophages or LPS-non-responsive macrophages. Combinations of LPS and tumor necrosis factor α (TNF-α) and macrophage-activating factor (MAF) were also used to stimulate the cells for NO production. The effect of VHSV on NO production depends on the response to LPS alone. When a low multiplicity of infection was used (1.78 x 10-3), the NO production in response to LPS in LPS-responsive macrophages was significantly decreased. However, LPS-non-responsive macrophage cultures produced NO when a combination of LPS and VHSV was used. In the case of a higher VHSV multiplicity of infection (1.78), no significant change was observed in LPS-non-responsive animals. Combinations of LPS with TNF- α, LPS with MAF, and TNF-α with MAF were used to induce NO production in LPS-non-responsive macrophages. In all these cases, VHSV suppressed NO production, although at a significant level only when a combination of TNF-α and MAF was used for the induction of NO. This research was supported by grant MAR 96-1775 from the Comisión Interministerial de Ciencia y Tecnología (CICYT) (Spain). C.T. acknowledges the Fundación Ramón Areces for a research fellowship. Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Diseases of Aquatic Organisms 47 101 107 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Nitric oxide Lipopolysacharide Macrophage Viral hemorrhagic virus Turbot Scophthalmus maximus Macrophage activating factor Tumor necrosis factor α |
spellingShingle |
Nitric oxide Lipopolysacharide Macrophage Viral hemorrhagic virus Turbot Scophthalmus maximus Macrophage activating factor Tumor necrosis factor α Tafalla, Carolina Figueras Huerta, Antonio Novoa, Beatriz Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production |
topic_facet |
Nitric oxide Lipopolysacharide Macrophage Viral hemorrhagic virus Turbot Scophthalmus maximus Macrophage activating factor Tumor necrosis factor α |
description |
7 pages, 4 figures. The effect of viral hemorrhagic septicemia virus (VHSV) in vitro infection on the nitric oxide (NO) production by turbot Scophthalmus maximus kidney macrophages has been addressed in the past. Previously, we had determined that only a small fraction of turbot possess head kidney macrophages that respond to a single exposure of lipopolysaccharide (LPS) with NO production (LPS-responsive macrophages), whereas macrophage cultures from other individuals were not activated by LPS alone and needed a combination of stimuli to respond (LPS-non-responsive macrophages). In the current work, we examined the effect of VHSV on NO production by macrophages characterized as LPS-responsive macrophages or LPS-non-responsive macrophages. Combinations of LPS and tumor necrosis factor α (TNF-α) and macrophage-activating factor (MAF) were also used to stimulate the cells for NO production. The effect of VHSV on NO production depends on the response to LPS alone. When a low multiplicity of infection was used (1.78 x 10-3), the NO production in response to LPS in LPS-responsive macrophages was significantly decreased. However, LPS-non-responsive macrophage cultures produced NO when a combination of LPS and VHSV was used. In the case of a higher VHSV multiplicity of infection (1.78), no significant change was observed in LPS-non-responsive animals. Combinations of LPS with TNF- α, LPS with MAF, and TNF-α with MAF were used to induce NO production in LPS-non-responsive macrophages. In all these cases, VHSV suppressed NO production, although at a significant level only when a combination of TNF-α and MAF was used for the induction of NO. This research was supported by grant MAR 96-1775 from the Comisión Interministerial de Ciencia y Tecnología (CICYT) (Spain). C.T. acknowledges the Fundación Ramón Areces for a research fellowship. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Tafalla, Carolina Figueras Huerta, Antonio Novoa, Beatriz |
author_facet |
Tafalla, Carolina Figueras Huerta, Antonio Novoa, Beatriz |
author_sort |
Tafalla, Carolina |
title |
Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production |
title_short |
Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production |
title_full |
Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production |
title_fullStr |
Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production |
title_full_unstemmed |
Viral hemorrhagic septicemia virus alters turbot Scophthalmus maximus macrophage nitric oxide production |
title_sort |
viral hemorrhagic septicemia virus alters turbot scophthalmus maximus macrophage nitric oxide production |
publisher |
Inter Research |
publishDate |
2001 |
url |
http://hdl.handle.net/10261/26266 https://doi.org/10.3354/dao047101 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.3354/dao047101 Diseases of Aquatic Organisms 47(2): 101-107 (2001) 0177-5103 http://hdl.handle.net/10261/26266 doi:10.3354/dao047101 |
op_rights |
open |
op_doi |
https://doi.org/10.3354/dao047101 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
47 |
container_start_page |
101 |
op_container_end_page |
107 |
_version_ |
1790607751759527936 |