Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes
© 2021 by the authors. Metamorphosis is a captivating process of change during which the morphology of the larva is completely reshaped to face the new challenges of adult life. In the case of fish, this process initiated in the brain has traditionally been considered to be a critical rearing point...
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Multidisciplinary Digital Publishing Institute
2021
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Online Access: | http://hdl.handle.net/10261/260368 https://doi.org/10.3390/biology10121256 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100010801 |
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ftcsic:oai:digital.csic.es:10261/260368 2024-02-11T10:09:16+01:00 Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes Guerrero-Peña, L. Suárez-Bregua, Paula Méndez-Martínez, Luis García-Fernández, Pablo Tur, Ricardo Rubiolo, Juan A. Tena, Juan J. Rotllant, Josep Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Xunta de Galicia 2021 http://hdl.handle.net/10261/260368 https://doi.org/10.3390/biology10121256 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100010801 unknown Multidisciplinary Digital Publishing Institute #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89648-P/ES/UN GENOMA PARA DOS DISEÑOS CORPORALES:ESTUDIOS DE GENOMICA FUNCIONAL Y TRANSCRIPTOMA A NIVEL DE GENOMA COMPLETO PARA COMPRENDER LAS BASES GENETICAS DE LA METAMORFOSIS EN PECES/ info:eu-repo/grantAgreement////PRE2018-085475 Publisher's version http://dx.doi.org/10.3390/biology10121256 Sí Biology 10(12): 1256 (2021) http://hdl.handle.net/10261/260368 doi:10.3390/biology10121256 2079-7737 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100010801 open Turbot Metamorphosis Brain RNA Sequencing Transcriptomes artículo http://purl.org/coar/resource_type/c_6501 2021 ftcsic https://doi.org/10.3390/biology1012125610.13039/50110001103310.13039/501100010801 2024-01-16T11:18:44Z © 2021 by the authors. Metamorphosis is a captivating process of change during which the morphology of the larva is completely reshaped to face the new challenges of adult life. In the case of fish, this process initiated in the brain has traditionally been considered to be a critical rearing point and despite the pioneering molecular work carried out in other flatfishes, the underlying molecular basis is still relatively poorly characterized. Turbot brain transcriptome of three developmental stages (pre-metamorphic, climax of metamorphosis and post-metamorphic) were analyzed to study the gene expression dynamics throughout the metamorphic process. A total of 1570 genes were differentially expressed in the three developmental stages and we found a specific pattern of gene expression at each stage. Unexpectedly, at the climax stage of metamorphosis, we found highly expressed genes related to the immune response, while the biological pathway enrichment analysis in pre-metamorphic and post-metamorphic were related to cell differentiation and oxygen carrier activity, respectively. In addition, our results confirm the importance of thyroid stimulating hormone, increasing its expression during metamorphosis. Based on our findings, we assume that immune system activation during the climax of metamorphosis stage could be related to processes of larval tissue inflammation, resorption and replacement, as occurs in other vertebrates. This research was funded by the MCIN/AEI/10.13039/501100011033 grant number AGL2017-89648P to JR and by “ERDF A way of making Europe”. L. Guerrero-Peña was supported by a pre-doctoral fellowship of the Spanish FPI Research Training Program funded by Spanish Economy and Competitiveness Ministry, grant number (PRE2018-085475). L. Méndez-Martínez was supported by a pre-doctoral fellowship of the Xunta de Galicia, grant number (IN606A-2020/006). Article in Journal/Newspaper Turbot Digital.CSIC (Spanish National Research Council) Martínez ENVELOPE(-62.183,-62.183,-64.650,-64.650) Biology 10 12 1256 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Turbot Metamorphosis Brain RNA Sequencing Transcriptomes |
spellingShingle |
Turbot Metamorphosis Brain RNA Sequencing Transcriptomes Guerrero-Peña, L. Suárez-Bregua, Paula Méndez-Martínez, Luis García-Fernández, Pablo Tur, Ricardo Rubiolo, Juan A. Tena, Juan J. Rotllant, Josep Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes |
topic_facet |
Turbot Metamorphosis Brain RNA Sequencing Transcriptomes |
description |
© 2021 by the authors. Metamorphosis is a captivating process of change during which the morphology of the larva is completely reshaped to face the new challenges of adult life. In the case of fish, this process initiated in the brain has traditionally been considered to be a critical rearing point and despite the pioneering molecular work carried out in other flatfishes, the underlying molecular basis is still relatively poorly characterized. Turbot brain transcriptome of three developmental stages (pre-metamorphic, climax of metamorphosis and post-metamorphic) were analyzed to study the gene expression dynamics throughout the metamorphic process. A total of 1570 genes were differentially expressed in the three developmental stages and we found a specific pattern of gene expression at each stage. Unexpectedly, at the climax stage of metamorphosis, we found highly expressed genes related to the immune response, while the biological pathway enrichment analysis in pre-metamorphic and post-metamorphic were related to cell differentiation and oxygen carrier activity, respectively. In addition, our results confirm the importance of thyroid stimulating hormone, increasing its expression during metamorphosis. Based on our findings, we assume that immune system activation during the climax of metamorphosis stage could be related to processes of larval tissue inflammation, resorption and replacement, as occurs in other vertebrates. This research was funded by the MCIN/AEI/10.13039/501100011033 grant number AGL2017-89648P to JR and by “ERDF A way of making Europe”. L. Guerrero-Peña was supported by a pre-doctoral fellowship of the Spanish FPI Research Training Program funded by Spanish Economy and Competitiveness Ministry, grant number (PRE2018-085475). L. Méndez-Martínez was supported by a pre-doctoral fellowship of the Xunta de Galicia, grant number (IN606A-2020/006). |
author2 |
Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Xunta de Galicia |
format |
Article in Journal/Newspaper |
author |
Guerrero-Peña, L. Suárez-Bregua, Paula Méndez-Martínez, Luis García-Fernández, Pablo Tur, Ricardo Rubiolo, Juan A. Tena, Juan J. Rotllant, Josep |
author_facet |
Guerrero-Peña, L. Suárez-Bregua, Paula Méndez-Martínez, Luis García-Fernández, Pablo Tur, Ricardo Rubiolo, Juan A. Tena, Juan J. Rotllant, Josep |
author_sort |
Guerrero-Peña, L. |
title |
Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes |
title_short |
Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes |
title_full |
Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes |
title_fullStr |
Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes |
title_full_unstemmed |
Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes |
title_sort |
brains in metamorphosis: temporal transcriptome dynamics in hatchery-reared flatfishes |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
http://hdl.handle.net/10261/260368 https://doi.org/10.3390/biology10121256 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100010801 |
long_lat |
ENVELOPE(-62.183,-62.183,-64.650,-64.650) |
geographic |
Martínez |
geographic_facet |
Martínez |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-89648-P/ES/UN GENOMA PARA DOS DISEÑOS CORPORALES:ESTUDIOS DE GENOMICA FUNCIONAL Y TRANSCRIPTOMA A NIVEL DE GENOMA COMPLETO PARA COMPRENDER LAS BASES GENETICAS DE LA METAMORFOSIS EN PECES/ info:eu-repo/grantAgreement////PRE2018-085475 Publisher's version http://dx.doi.org/10.3390/biology10121256 Sí Biology 10(12): 1256 (2021) http://hdl.handle.net/10261/260368 doi:10.3390/biology10121256 2079-7737 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100010801 |
op_rights |
open |
op_doi |
https://doi.org/10.3390/biology1012125610.13039/50110001103310.13039/501100010801 |
container_title |
Biology |
container_volume |
10 |
container_issue |
12 |
container_start_page |
1256 |
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1790609090084339712 |