Brains in Metamorphosis: Temporal Transcriptome Dynamics in Hatchery-Reared Flatfishes

• Published in: Biology
• Main Authors: Guerrero-Peña, L., Suárez-Bregua, Paula, Méndez-Martínez, Luis, García-Fernández, Pablo, Tur, Ricardo, Rubiolo, Juan A., Tena, Juan J., Rotllant, Josep
• Other Authors: Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Xunta de Galicia
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: Multidisciplinary Digital Publishing Institute 2021
• Subjects: Turbot; Metamorphosis; Brain; RNA; Sequencing; Transcriptomes; Martínez
• Online Access: http://hdl.handle.net/10261/260368; https://doi.org/10.3390/biology10121256; https://doi.org/10.13039/501100011033; https://doi.org/10.13039/501100010801

© 2021 by the authors. Metamorphosis is a captivating process of change during which the morphology of the larva is completely reshaped to face the new challenges of adult life. In the case of fish, this process initiated in the brain has traditionally been considered to be a critical rearing point and despite the pioneering molecular work carried out in other flatfishes, the underlying molecular basis is still relatively poorly characterized. Turbot brain transcriptome of three developmental stages (pre-metamorphic, climax of metamorphosis and post-metamorphic) were analyzed to study the gene expression dynamics throughout the metamorphic process. A total of 1570 genes were differentially expressed in the three developmental stages and we found a specific pattern of gene expression at each stage. Unexpectedly, at the climax stage of metamorphosis, we found highly expressed genes related to the immune response, while the biological pathway enrichment analysis in pre-metamorphic and post-metamorphic were related to cell differentiation and oxygen carrier activity, respectively. In addition, our results confirm the importance of thyroid stimulating hormone, increasing its expression during metamorphosis. Based on our findings, we assume that immune system activation during the climax of metamorphosis stage could be related to processes of larval tissue inflammation, resorption and replacement, as occurs in other vertebrates. This research was funded by the MCIN/AEI/10.13039/501100011033 grant number AGL2017-89648P to JR and by “ERDF A way of making Europe”. L. Guerrero-Peña was supported by a pre-doctoral fellowship of the Spanish FPI Research Training Program funded by Spanish Economy and Competitiveness Ministry, grant number (PRE2018-085475). L. Méndez-Martínez was supported by a pre-doctoral fellowship of the Xunta de Galicia, grant number (IN606A-2020/006).