Compound-Specific Stable Isotope Analysis of Amino Acids in Pelagic Shark Vertebrae Reveals Baseline, Trophic, and Physiological Effects on Bulk Protein Isotope Records
17 pages, 6 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) Variations in stable carbon and nitrogen isotope compositions in incremental tissues of pelagic sharks can be used to infer aspects of their spatial and trop...
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Online Access: | http://hdl.handle.net/10261/258635 https://doi.org/10.3389/fmars.2021.673016 |
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ftcsic:oai:digital.csic.es:10261/258635 2024-02-11T10:06:45+01:00 Compound-Specific Stable Isotope Analysis of Amino Acids in Pelagic Shark Vertebrae Reveals Baseline, Trophic, and Physiological Effects on Bulk Protein Isotope Records Magozzi, Sarah Thorrold, Simon R. Houghton, Leah Bendall, Victoria A. Hetherington, Stuart Mucientes, Gonzalo Natanson, Lisa J. Queiroz, Nuno Santos, Miguel N. Trueman, Clive N. 2021 http://hdl.handle.net/10261/258635 https://doi.org/10.3389/fmars.2021.673016 en eng Frontiers Media Publisher's version https://doi.org/10.3389/fmars.2021.673016 Sí Frontiers in Marine Science 8: 673016 (2021) http://hdl.handle.net/10261/258635 doi:10.3389/fmars.2021.673016 2296-7745 open artículo http://purl.org/coar/resource_type/c_6501 2021 ftcsic https://doi.org/10.3389/fmars.2021.673016 2024-01-16T11:17:50Z 17 pages, 6 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) Variations in stable carbon and nitrogen isotope compositions in incremental tissues of pelagic sharks can be used to infer aspects of their spatial and trophic ecology across life-histories. Interpretations from bulk tissue isotopic compositions are complicated, however, because multiple processes influence these values, including variations in primary producer isotope ratios and consumer diets and physiological processing of metabolites. Here we challenge inferences about shark tropho-spatial ecology drawn from bulk tissue isotope data using data for amino acids. Stable isotope compositions of individual amino acids can partition the isotopic variance in bulk tissue into components associated with primary production on the one hand, and diet and physiology on the other. The carbon framework of essential amino acids (EAAs) can be synthesised de novo only by plants, fungi and bacteria and must be acquired by consumers through the diet. Consequently, the carbon isotopic composition of EAAs in consumers reflects that of primary producers in the location of feeding, whereas that of non-essential amino acids (non-EAAs) is additionally influenced by trophic fractionation and isotope dynamics of metabolic processing. We determined isotope chronologies from vertebrae of individual blue sharks and porbeagles from the North Atlantic. We measured carbon and nitrogen isotope compositions in bulk collagen and carbon isotope compositions of amino acids. Despite variability among individuals, common ontogenetic patterns in bulk isotope compositions were seen in both species. However, while life-history movement inferences from bulk analyses for blue sharks were supported by carbon isotope data from essential amino acids, inferences for porbeagles were not, implying that the observed trends in bulk protein isotope compositions in porbeagles have a trophic or physiological explanation, ... Article in Journal/Newspaper North Atlantic Digital.CSIC (Spanish National Research Council) Frontiers in Marine Science 8 |
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Digital.CSIC (Spanish National Research Council) |
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ftcsic |
language |
English |
description |
17 pages, 6 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) Variations in stable carbon and nitrogen isotope compositions in incremental tissues of pelagic sharks can be used to infer aspects of their spatial and trophic ecology across life-histories. Interpretations from bulk tissue isotopic compositions are complicated, however, because multiple processes influence these values, including variations in primary producer isotope ratios and consumer diets and physiological processing of metabolites. Here we challenge inferences about shark tropho-spatial ecology drawn from bulk tissue isotope data using data for amino acids. Stable isotope compositions of individual amino acids can partition the isotopic variance in bulk tissue into components associated with primary production on the one hand, and diet and physiology on the other. The carbon framework of essential amino acids (EAAs) can be synthesised de novo only by plants, fungi and bacteria and must be acquired by consumers through the diet. Consequently, the carbon isotopic composition of EAAs in consumers reflects that of primary producers in the location of feeding, whereas that of non-essential amino acids (non-EAAs) is additionally influenced by trophic fractionation and isotope dynamics of metabolic processing. We determined isotope chronologies from vertebrae of individual blue sharks and porbeagles from the North Atlantic. We measured carbon and nitrogen isotope compositions in bulk collagen and carbon isotope compositions of amino acids. Despite variability among individuals, common ontogenetic patterns in bulk isotope compositions were seen in both species. However, while life-history movement inferences from bulk analyses for blue sharks were supported by carbon isotope data from essential amino acids, inferences for porbeagles were not, implying that the observed trends in bulk protein isotope compositions in porbeagles have a trophic or physiological explanation, ... |
format |
Article in Journal/Newspaper |
author |
Magozzi, Sarah Thorrold, Simon R. Houghton, Leah Bendall, Victoria A. Hetherington, Stuart Mucientes, Gonzalo Natanson, Lisa J. Queiroz, Nuno Santos, Miguel N. Trueman, Clive N. |
spellingShingle |
Magozzi, Sarah Thorrold, Simon R. Houghton, Leah Bendall, Victoria A. Hetherington, Stuart Mucientes, Gonzalo Natanson, Lisa J. Queiroz, Nuno Santos, Miguel N. Trueman, Clive N. Compound-Specific Stable Isotope Analysis of Amino Acids in Pelagic Shark Vertebrae Reveals Baseline, Trophic, and Physiological Effects on Bulk Protein Isotope Records |
author_facet |
Magozzi, Sarah Thorrold, Simon R. Houghton, Leah Bendall, Victoria A. Hetherington, Stuart Mucientes, Gonzalo Natanson, Lisa J. Queiroz, Nuno Santos, Miguel N. Trueman, Clive N. |
author_sort |
Magozzi, Sarah |
title |
Compound-Specific Stable Isotope Analysis of Amino Acids in Pelagic Shark Vertebrae Reveals Baseline, Trophic, and Physiological Effects on Bulk Protein Isotope Records |
title_short |
Compound-Specific Stable Isotope Analysis of Amino Acids in Pelagic Shark Vertebrae Reveals Baseline, Trophic, and Physiological Effects on Bulk Protein Isotope Records |
title_full |
Compound-Specific Stable Isotope Analysis of Amino Acids in Pelagic Shark Vertebrae Reveals Baseline, Trophic, and Physiological Effects on Bulk Protein Isotope Records |
title_fullStr |
Compound-Specific Stable Isotope Analysis of Amino Acids in Pelagic Shark Vertebrae Reveals Baseline, Trophic, and Physiological Effects on Bulk Protein Isotope Records |
title_full_unstemmed |
Compound-Specific Stable Isotope Analysis of Amino Acids in Pelagic Shark Vertebrae Reveals Baseline, Trophic, and Physiological Effects on Bulk Protein Isotope Records |
title_sort |
compound-specific stable isotope analysis of amino acids in pelagic shark vertebrae reveals baseline, trophic, and physiological effects on bulk protein isotope records |
publisher |
Frontiers Media |
publishDate |
2021 |
url |
http://hdl.handle.net/10261/258635 https://doi.org/10.3389/fmars.2021.673016 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Publisher's version https://doi.org/10.3389/fmars.2021.673016 Sí Frontiers in Marine Science 8: 673016 (2021) http://hdl.handle.net/10261/258635 doi:10.3389/fmars.2021.673016 2296-7745 |
op_rights |
open |
op_doi |
https://doi.org/10.3389/fmars.2021.673016 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
_version_ |
1790604659374686208 |