Leukocyte counts in blood smears of Antarctic seals and penguins: a new less time-consuming method
Research on immune response in polar fauna is gaining great importance due to different scenarios of environmental change. Total leukocyte counts in blood smears are one of the most widespread practices and provide useful information about the health status of individuals. However, there is no metho...
Published in: | Polar Biology |
---|---|
Main Authors: | , , , , , , |
Other Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Springer Nature
2021
|
Subjects: | |
Online Access: | http://hdl.handle.net/10261/256788 https://doi.org/10.1007/s00300-021-02950-0 https://doi.org/10.13039/501100010964 https://doi.org/10.13039/501100003329 |
id |
ftcsic:oai:digital.csic.es:10261/256788 |
---|---|
record_format |
openpolar |
spelling |
ftcsic:oai:digital.csic.es:10261/256788 2024-02-11T09:58:25+01:00 Leukocyte counts in blood smears of Antarctic seals and penguins: a new less time-consuming method Menéndez-Blázquez, Javier Soto, Florencia Negrete, Javier Colominas-Ciuró, Roger Marín-Sierra, Andrea Ricca, Melina Barbosa, Andrés Ministerio de Economía y Competitividad (España) Instituto Antártico Argentino 2021-10-04 http://hdl.handle.net/10261/256788 https://doi.org/10.1007/s00300-021-02950-0 https://doi.org/10.13039/501100010964 https://doi.org/10.13039/501100003329 unknown Springer Nature #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//CTM2011-24427/ES/PARASITOS, RESPUESTA INMUNE Y ESTRES OXIDATIVO EN PINGUINOS ANTARTICOS: CONTRIBUCION AL CONOCIMIENTO DE LOS EFECTOS DEL CAMBIO GLOBAL (PINGUCLIM III)/ info:eu-repo/grantAgreement/MINECO//CTM2015-64720-R/ES/RESPUESTA INMUNE, PARASITISMO, CONTAMINANTES Y BUSQUEDA DEL ALIMENTO EN PINGUINOS ANTARTICOS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108597RB-I00/ES/CONSECUENCIAS ECOLOGICAS Y EVOLUTIVAS DE LA PERSONALIDAD EN PINGUINOS ANTARTICOS EN UN MUNDO CAMBIANTE/ http://dx.doi.org/10.1007/s00300-021-02950-0 Sí doi:10.1007/s00300-021-02950-0 issn: 1432-2056 Polar Biology 44: 2195-2198 (2021) http://hdl.handle.net/10261/256788 http://dx.doi.org/10.13039/501100010964 http://dx.doi.org/10.13039/501100003329 none Cell counts Cellular immune response Marine birds Marine mammals Methodology artículo http://purl.org/coar/resource_type/c_6501 2021 ftcsic https://doi.org/10.1007/s00300-021-02950-010.13039/50110001096410.13039/501100003329 2024-01-16T11:16:52Z Research on immune response in polar fauna is gaining great importance due to different scenarios of environmental change. Total leukocyte counts in blood smears are one of the most widespread practices and provide useful information about the health status of individuals. However, there is no methodological agreement for these analyses. Total leukocyte counts can be performed at ×400 magnification in optical microscopy using 10,000 erythrocytes for standardizing. However, counting such number of erythrocytes is costly and time-consuming. Here, we describe a new technique to simplify leukocyte counts in blood smears from Antarctic wildlife based on the number of microscope fields instead of the number of erythrocytes which reduces considerably the time spent. We have counted total leukocytes using both methods in the three penguin species of the genus Pygoscelis—Adélie (P. adeliae), gentoo (P. papua), and chinstrap penguin (P. antarcticus)—and four Antarctic mammal species: crabeater seal (Lobodon carcinophaga), leopard seal (Hydrurga leptonyx), weddell seal (Leptonychotes weddellii), and southern elephant seal (Mirounga leonina) for validation. Our results show a high correlation between the total leukocyte counts using 10,000 erythrocytes or 10 microscope fields for standardizing. These results show the reliability of the latter method for counting the total number of leukocytes in different species of birds and mammals saving time and effort. The study was funded by the Spanish Ministry of Economy and Competitiveness through projects CTM2011-24427, CTM2015-64720, and PID2019-108597 and by the Dirección Nacional del Antártico, Instituto Antártico Argentino (PICTA 2010-01). Article in Journal/Newspaper Antarc* Antarctic antarcticus Chinstrap penguin Crabeater Seal Dirección Nacional del Antártico Elephant Seal Hydrurga leptonyx Instituto Antártico Argentino Leopard Seal Mirounga leonina Polar Biology Southern Elephant Seal Weddell Seal Digital.CSIC (Spanish National Research Council) Antarctic Argentino Hydrurga ENVELOPE(-61.626,-61.626,-64.145,-64.145) Weddell Polar Biology 44 11 2195 2198 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Cell counts Cellular immune response Marine birds Marine mammals Methodology |
spellingShingle |
Cell counts Cellular immune response Marine birds Marine mammals Methodology Menéndez-Blázquez, Javier Soto, Florencia Negrete, Javier Colominas-Ciuró, Roger Marín-Sierra, Andrea Ricca, Melina Barbosa, Andrés Leukocyte counts in blood smears of Antarctic seals and penguins: a new less time-consuming method |
topic_facet |
Cell counts Cellular immune response Marine birds Marine mammals Methodology |
description |
Research on immune response in polar fauna is gaining great importance due to different scenarios of environmental change. Total leukocyte counts in blood smears are one of the most widespread practices and provide useful information about the health status of individuals. However, there is no methodological agreement for these analyses. Total leukocyte counts can be performed at ×400 magnification in optical microscopy using 10,000 erythrocytes for standardizing. However, counting such number of erythrocytes is costly and time-consuming. Here, we describe a new technique to simplify leukocyte counts in blood smears from Antarctic wildlife based on the number of microscope fields instead of the number of erythrocytes which reduces considerably the time spent. We have counted total leukocytes using both methods in the three penguin species of the genus Pygoscelis—Adélie (P. adeliae), gentoo (P. papua), and chinstrap penguin (P. antarcticus)—and four Antarctic mammal species: crabeater seal (Lobodon carcinophaga), leopard seal (Hydrurga leptonyx), weddell seal (Leptonychotes weddellii), and southern elephant seal (Mirounga leonina) for validation. Our results show a high correlation between the total leukocyte counts using 10,000 erythrocytes or 10 microscope fields for standardizing. These results show the reliability of the latter method for counting the total number of leukocytes in different species of birds and mammals saving time and effort. The study was funded by the Spanish Ministry of Economy and Competitiveness through projects CTM2011-24427, CTM2015-64720, and PID2019-108597 and by the Dirección Nacional del Antártico, Instituto Antártico Argentino (PICTA 2010-01). |
author2 |
Ministerio de Economía y Competitividad (España) Instituto Antártico Argentino |
format |
Article in Journal/Newspaper |
author |
Menéndez-Blázquez, Javier Soto, Florencia Negrete, Javier Colominas-Ciuró, Roger Marín-Sierra, Andrea Ricca, Melina Barbosa, Andrés |
author_facet |
Menéndez-Blázquez, Javier Soto, Florencia Negrete, Javier Colominas-Ciuró, Roger Marín-Sierra, Andrea Ricca, Melina Barbosa, Andrés |
author_sort |
Menéndez-Blázquez, Javier |
title |
Leukocyte counts in blood smears of Antarctic seals and penguins: a new less time-consuming method |
title_short |
Leukocyte counts in blood smears of Antarctic seals and penguins: a new less time-consuming method |
title_full |
Leukocyte counts in blood smears of Antarctic seals and penguins: a new less time-consuming method |
title_fullStr |
Leukocyte counts in blood smears of Antarctic seals and penguins: a new less time-consuming method |
title_full_unstemmed |
Leukocyte counts in blood smears of Antarctic seals and penguins: a new less time-consuming method |
title_sort |
leukocyte counts in blood smears of antarctic seals and penguins: a new less time-consuming method |
publisher |
Springer Nature |
publishDate |
2021 |
url |
http://hdl.handle.net/10261/256788 https://doi.org/10.1007/s00300-021-02950-0 https://doi.org/10.13039/501100010964 https://doi.org/10.13039/501100003329 |
long_lat |
ENVELOPE(-61.626,-61.626,-64.145,-64.145) |
geographic |
Antarctic Argentino Hydrurga Weddell |
geographic_facet |
Antarctic Argentino Hydrurga Weddell |
genre |
Antarc* Antarctic antarcticus Chinstrap penguin Crabeater Seal Dirección Nacional del Antártico Elephant Seal Hydrurga leptonyx Instituto Antártico Argentino Leopard Seal Mirounga leonina Polar Biology Southern Elephant Seal Weddell Seal |
genre_facet |
Antarc* Antarctic antarcticus Chinstrap penguin Crabeater Seal Dirección Nacional del Antártico Elephant Seal Hydrurga leptonyx Instituto Antártico Argentino Leopard Seal Mirounga leonina Polar Biology Southern Elephant Seal Weddell Seal |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//CTM2011-24427/ES/PARASITOS, RESPUESTA INMUNE Y ESTRES OXIDATIVO EN PINGUINOS ANTARTICOS: CONTRIBUCION AL CONOCIMIENTO DE LOS EFECTOS DEL CAMBIO GLOBAL (PINGUCLIM III)/ info:eu-repo/grantAgreement/MINECO//CTM2015-64720-R/ES/RESPUESTA INMUNE, PARASITISMO, CONTAMINANTES Y BUSQUEDA DEL ALIMENTO EN PINGUINOS ANTARTICOS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108597RB-I00/ES/CONSECUENCIAS ECOLOGICAS Y EVOLUTIVAS DE LA PERSONALIDAD EN PINGUINOS ANTARTICOS EN UN MUNDO CAMBIANTE/ http://dx.doi.org/10.1007/s00300-021-02950-0 Sí doi:10.1007/s00300-021-02950-0 issn: 1432-2056 Polar Biology 44: 2195-2198 (2021) http://hdl.handle.net/10261/256788 http://dx.doi.org/10.13039/501100010964 http://dx.doi.org/10.13039/501100003329 |
op_rights |
none |
op_doi |
https://doi.org/10.1007/s00300-021-02950-010.13039/50110001096410.13039/501100003329 |
container_title |
Polar Biology |
container_volume |
44 |
container_issue |
11 |
container_start_page |
2195 |
op_container_end_page |
2198 |
_version_ |
1790594050486697984 |