Enteromyxum leei and E. scophthalmi (Myxozoa) induce modifications in the intestinal carbohydrate patterns of their host, Sparus aurata L. amd Psetta maxima

• Main Authors: Redondo, Mª José, Álvarez-Pellitero, Mª del Pilar
• Other Authors: Ministerio de Educación y Ciencia (España), Consejo Superior de Investigaciones Científicas (España), European Commission
• Format: Still Image
• Language: unknown
• Published: 2009
• Subjects: Turbot
• Online Access: http://hdl.handle.net/10261/255107; https://doi.org/10.13039/501100003339; https://doi.org/10.13039/501100000780

Resumen del trabajo presentado en la 14th EAFP International Conference (European Association of Fish Pathologists), celebrada en Praga (República Checa), del 14 al 19 de septiembre de 2009 Enteromyxum leei and Enteromyxum scophthalmi are myxosporean parasites which produces serious losses in sparids and turbot aquaculture, respectively (1, 2). Both myxozoans show a high affinity for the digestive tract, the target organ. Mucins and glycoconjugates present at the mucosal epithelium playa significant role in the host¬parasite interactions. In the current work, the carbohydrate terminals present in the intestine of E. leei-infected gilthead sea bream (GSB) and E. scophthalmi-infected tur¬bot (TB) were studied in comparison with uninfected fish. Lectin staining (see Table) and other histochemical techniques for carbohydrate detection (PAS, alcian blue, PAS/ alcian blue) were used. The mucin pattern in goblet cells was the contrary for both fish species, with acidic mucins in the intestine of GSB and the esophagus of TB and neutral mucins in sorne parts of the GSB esophagus and the TB intestine. However, no differences were observed between infected and uninfected fish regarding the presence of different types of mucins and glycogen in the intestinal epithelium. In contrast, clear differences in the presence and abundance of the different carbohydrate terminals were observed in infected fish with respect to uninfected fish and also according to the considered host/ parasite model. In comparison to uninfected fish, fucose terminals clearly decreased in E. leei-infected GSB (both in the epithelial cells and in the brush border) and they were slightly less expressed in E. scophtlami-infected TB. Similarly, N-acetyl-glucosamine and glusose/mannose terminals diminished in both infected fish, but the decreasing was especially evident in E. leei-infected GSB. N-acetyl -neuraminic acid, which was present in uninfected GSB, was not found in E. leei-infected fish. This influence of Enteromyxum spp. infection on the ...