In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus
In vitro cultivation of the myxozoan Enteromyxum scophthalmi was attempted using different culture media and conditions. The progress of the cultures was monitored using dye-exclusion viability counts, tetrazolium-based cell-proliferation assays, measuring the incorporation of BrdU during DNA synthe...
Published in: | Diseases of Aquatic Organisms |
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Inter Research
2003
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Online Access: | http://hdl.handle.net/10261/253781 https://doi.org/10.3354/dao055133 https://doi.org/10.13039/501100000780 |
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ftcsic:oai:digital.csic.es:10261/253781 2024-02-11T10:08:27+01:00 In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus Redondo, Mª José Palenzuela, Oswaldo Álvarez-Pellitero, Mª del Pilar European Commission Stolt Sea Farm 2003 http://hdl.handle.net/10261/253781 https://doi.org/10.3354/dao055133 https://doi.org/10.13039/501100000780 unknown Inter Research http://dx.doi.org/10.3354/dao055133 Sí doi:10.3354/dao055133 issn: 0177-5103 e-issn: 1616-1580 Diseases of Aquatic Organisms 55(2): 133-144 (2003) http://hdl.handle.net/10261/253781 http://dx.doi.org/10.13039/501100000780 none In vitro cultivation Life cycle Myxozoa Parasites artículo http://purl.org/coar/resource_type/c_6501 2003 ftcsic https://doi.org/10.3354/dao05513310.13039/501100000780 2024-01-16T11:15:18Z In vitro cultivation of the myxozoan Enteromyxum scophthalmi was attempted using different culture media and conditions. The progress of the cultures was monitored using dye-exclusion viability counts, tetrazolium-based cell-proliferation assays, measuring the incorporation of BrdU during DNA synthesis, and by morphological studies using light and electron microscopes. In preliminary experiments, the persistence of viable stages for a few days was ascertained in both medium 199 (M199) and in seawater. An apparent initial proliferation was noticed in the culture media, with many young stages observed by Day 7 post-inoculation (p.i.). In contrast, fast degeneration occurred in seawater, with but a few living stages persisting to Day 1 p.i and none to Day 5 p.i. Both tetrazolium-based cell-proliferation assays and dye-exclusion viability counts demonstrated a progressive degeneration of the cultures. Although M199 medium and neutral pH with the addition of sera appeared to provide the most favourable conditions during the first few hours, all cultures degenerated with time and no parasite proliferation or maintenance could be achieved in the long term in any of the conditions assayed, including attempts of co-cultivation with a turbot cell line. The ultrastructure of stages cultured for 15 d demonstrated complete degeneration of organelles and mitochondria, although the plasma membrane remained intact in many stages. Unknown factors related to the metabolism or the life cycle of this myxozoan are probably responsible for the inability to culture the parasite, which seems to be strictly dependent on the target host tissues for survival. This study was financed by the EU and Spanish Government through the research grant FEDER 1FD97-0679-C02-01. Additional support was provided by Stolt Sea Farm, S.A. The authors are thankful to the Electron Microscopy Services from the Universities of Barcelona and Valencia. Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Diseases of Aquatic Organisms 55 133 144 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
In vitro cultivation Life cycle Myxozoa Parasites |
spellingShingle |
In vitro cultivation Life cycle Myxozoa Parasites Redondo, Mª José Palenzuela, Oswaldo Álvarez-Pellitero, Mª del Pilar In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus |
topic_facet |
In vitro cultivation Life cycle Myxozoa Parasites |
description |
In vitro cultivation of the myxozoan Enteromyxum scophthalmi was attempted using different culture media and conditions. The progress of the cultures was monitored using dye-exclusion viability counts, tetrazolium-based cell-proliferation assays, measuring the incorporation of BrdU during DNA synthesis, and by morphological studies using light and electron microscopes. In preliminary experiments, the persistence of viable stages for a few days was ascertained in both medium 199 (M199) and in seawater. An apparent initial proliferation was noticed in the culture media, with many young stages observed by Day 7 post-inoculation (p.i.). In contrast, fast degeneration occurred in seawater, with but a few living stages persisting to Day 1 p.i and none to Day 5 p.i. Both tetrazolium-based cell-proliferation assays and dye-exclusion viability counts demonstrated a progressive degeneration of the cultures. Although M199 medium and neutral pH with the addition of sera appeared to provide the most favourable conditions during the first few hours, all cultures degenerated with time and no parasite proliferation or maintenance could be achieved in the long term in any of the conditions assayed, including attempts of co-cultivation with a turbot cell line. The ultrastructure of stages cultured for 15 d demonstrated complete degeneration of organelles and mitochondria, although the plasma membrane remained intact in many stages. Unknown factors related to the metabolism or the life cycle of this myxozoan are probably responsible for the inability to culture the parasite, which seems to be strictly dependent on the target host tissues for survival. This study was financed by the EU and Spanish Government through the research grant FEDER 1FD97-0679-C02-01. Additional support was provided by Stolt Sea Farm, S.A. The authors are thankful to the Electron Microscopy Services from the Universities of Barcelona and Valencia. |
author2 |
European Commission Stolt Sea Farm |
format |
Article in Journal/Newspaper |
author |
Redondo, Mª José Palenzuela, Oswaldo Álvarez-Pellitero, Mª del Pilar |
author_facet |
Redondo, Mª José Palenzuela, Oswaldo Álvarez-Pellitero, Mª del Pilar |
author_sort |
Redondo, Mª José |
title |
In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus |
title_short |
In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus |
title_full |
In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus |
title_fullStr |
In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus |
title_full_unstemmed |
In vitro studies on viability and proliferation of Enteromyxum scophthalmi (Myxozoa), an enteric parasite of cultured turbot Scophthalmus maximus |
title_sort |
in vitro studies on viability and proliferation of enteromyxum scophthalmi (myxozoa), an enteric parasite of cultured turbot scophthalmus maximus |
publisher |
Inter Research |
publishDate |
2003 |
url |
http://hdl.handle.net/10261/253781 https://doi.org/10.3354/dao055133 https://doi.org/10.13039/501100000780 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.3354/dao055133 Sí doi:10.3354/dao055133 issn: 0177-5103 e-issn: 1616-1580 Diseases of Aquatic Organisms 55(2): 133-144 (2003) http://hdl.handle.net/10261/253781 http://dx.doi.org/10.13039/501100000780 |
op_rights |
none |
op_doi |
https://doi.org/10.3354/dao05513310.13039/501100000780 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
55 |
container_start_page |
133 |
op_container_end_page |
144 |
_version_ |
1790607806042210304 |