Cryptosporidium scophthalmi n. sp. (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study
Cryptosporidium scophthalmi n. sp. is described from the turbot Scophthalmus maximus L., sampled from different farms on the coast of NW Spain. The parasite was found mainly in the intestinal epithelium and very seldom in the stomach. Oocysts were almost spherical, with 4 naked sporozoites and a res...
Published in: | Diseases of Aquatic Organisms |
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Format: | Article in Journal/Newspaper |
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Inter Research
2004
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Online Access: | http://hdl.handle.net/10261/253764 https://doi.org/10.3354/dao062133 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100006280 |
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ftcsic:oai:digital.csic.es:10261/253764 2024-02-11T10:08:26+01:00 Cryptosporidium scophthalmi n. sp. (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study Álvarez-Pellitero, Mª del Pilar Quiroga, María Isabel Sitjà-Bobadilla, Ariadna Redondo, Mª José Palenzuela, Oswaldo Padrós, Francesc Vázquez, Sonia Nieto, José M. European Commission Ministerio de Ciencia y Tecnología (España) Stolt Sea Farm 2004 http://hdl.handle.net/10261/253764 https://doi.org/10.3354/dao062133 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100006280 unknown Inter Research http://dx.doi.org/10.3354/dao062133 Sí doi:10.3354/dao062133 issn: 0177-5103 e-issn: 1616-1580 Diseases of Aquatic Organisms 62(1-2): 133-145 (2004) http://hdl.handle.net/10261/253764 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100006280 none Aquaculture Coccidia Cryptosporidium scophthalmi n. sp Histopathology Teleostei Ultrastructure artículo http://purl.org/coar/resource_type/c_6501 2004 ftcsic https://doi.org/10.3354/dao06213310.13039/50110000078010.13039/501100006280 2024-01-16T11:15:18Z Cryptosporidium scophthalmi n. sp. is described from the turbot Scophthalmus maximus L., sampled from different farms on the coast of NW Spain. The parasite was found mainly in the intestinal epithelium and very seldom in the stomach. Oocysts were almost spherical, with 4 naked sporozoites and a residuum, and measured 3.7-5.03 x 3.03-4.69 μm (mean 4.44 x 3.91) (shape index 1.05-1.34, mean 1.14). Sporulation was endogenous, as fully sporulated oocysts were found within the intestinal epithelium, lumen and faeces. Merogonial and gamogonial stages were in the typical extracytoplasmic position, whereas sporogonial stages were deep within the epithelium. Oocysts and other stages of C. scophthalmi comply with most of the diagnostic features of the genus Cryptosporidium, but differ from all hitherto described species. Ultrastructural features, including the characteristic feeding organelle, were mainly comparable with those of other Cryptosporidium species. Mitochondria were frequently observed in sporozoites. Infection prevalence was very variable, and juvenile fish were most frequently and intensively parasitised. External clinical signs were not detected, although some fish showed intestinal distension at necropsy. The marked histopathological damage occurring in severe infection includes distension of epithelial cells by large vacuoles, containing clusters of oocysts, and can lead to sloughing of epithelial cell remnants and oocysts or even detachment of intestinal mucosa. An inflammatory reaction involving leucocyte infiltration was sometimes observed. Funding for this study was provided by the EU and the Spanish Ministerio de Ciencia y Tecnología through research grants FEDER 1FD97-0679 and AGL2001- 2241. Additional support was obtained from Stolt Sea Farm S. A. We are thankful to technical services at the Universities of Barcelona and Valencia, to J. Monfort from IATS, and to M. C. Carreira Valle from the School of Veterinary Medicine of Lugo (University of Santiago) for assistance in processing electron and ... Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Diseases of Aquatic Organisms 62 133 145 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Aquaculture Coccidia Cryptosporidium scophthalmi n. sp Histopathology Teleostei Ultrastructure |
spellingShingle |
Aquaculture Coccidia Cryptosporidium scophthalmi n. sp Histopathology Teleostei Ultrastructure Álvarez-Pellitero, Mª del Pilar Quiroga, María Isabel Sitjà-Bobadilla, Ariadna Redondo, Mª José Palenzuela, Oswaldo Padrós, Francesc Vázquez, Sonia Nieto, José M. Cryptosporidium scophthalmi n. sp. (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study |
topic_facet |
Aquaculture Coccidia Cryptosporidium scophthalmi n. sp Histopathology Teleostei Ultrastructure |
description |
Cryptosporidium scophthalmi n. sp. is described from the turbot Scophthalmus maximus L., sampled from different farms on the coast of NW Spain. The parasite was found mainly in the intestinal epithelium and very seldom in the stomach. Oocysts were almost spherical, with 4 naked sporozoites and a residuum, and measured 3.7-5.03 x 3.03-4.69 μm (mean 4.44 x 3.91) (shape index 1.05-1.34, mean 1.14). Sporulation was endogenous, as fully sporulated oocysts were found within the intestinal epithelium, lumen and faeces. Merogonial and gamogonial stages were in the typical extracytoplasmic position, whereas sporogonial stages were deep within the epithelium. Oocysts and other stages of C. scophthalmi comply with most of the diagnostic features of the genus Cryptosporidium, but differ from all hitherto described species. Ultrastructural features, including the characteristic feeding organelle, were mainly comparable with those of other Cryptosporidium species. Mitochondria were frequently observed in sporozoites. Infection prevalence was very variable, and juvenile fish were most frequently and intensively parasitised. External clinical signs were not detected, although some fish showed intestinal distension at necropsy. The marked histopathological damage occurring in severe infection includes distension of epithelial cells by large vacuoles, containing clusters of oocysts, and can lead to sloughing of epithelial cell remnants and oocysts or even detachment of intestinal mucosa. An inflammatory reaction involving leucocyte infiltration was sometimes observed. Funding for this study was provided by the EU and the Spanish Ministerio de Ciencia y Tecnología through research grants FEDER 1FD97-0679 and AGL2001- 2241. Additional support was obtained from Stolt Sea Farm S. A. We are thankful to technical services at the Universities of Barcelona and Valencia, to J. Monfort from IATS, and to M. C. Carreira Valle from the School of Veterinary Medicine of Lugo (University of Santiago) for assistance in processing electron and ... |
author2 |
European Commission Ministerio de Ciencia y Tecnología (España) Stolt Sea Farm |
format |
Article in Journal/Newspaper |
author |
Álvarez-Pellitero, Mª del Pilar Quiroga, María Isabel Sitjà-Bobadilla, Ariadna Redondo, Mª José Palenzuela, Oswaldo Padrós, Francesc Vázquez, Sonia Nieto, José M. |
author_facet |
Álvarez-Pellitero, Mª del Pilar Quiroga, María Isabel Sitjà-Bobadilla, Ariadna Redondo, Mª José Palenzuela, Oswaldo Padrós, Francesc Vázquez, Sonia Nieto, José M. |
author_sort |
Álvarez-Pellitero, Mª del Pilar |
title |
Cryptosporidium scophthalmi n. sp. (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study |
title_short |
Cryptosporidium scophthalmi n. sp. (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study |
title_full |
Cryptosporidium scophthalmi n. sp. (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study |
title_fullStr |
Cryptosporidium scophthalmi n. sp. (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study |
title_full_unstemmed |
Cryptosporidium scophthalmi n. sp. (Apicomplexa: Cryptosporidiidae) from cultured turbot Scophthalmus maximus. Light and electron microscope description and histopathological study |
title_sort |
cryptosporidium scophthalmi n. sp. (apicomplexa: cryptosporidiidae) from cultured turbot scophthalmus maximus. light and electron microscope description and histopathological study |
publisher |
Inter Research |
publishDate |
2004 |
url |
http://hdl.handle.net/10261/253764 https://doi.org/10.3354/dao062133 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100006280 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.3354/dao062133 Sí doi:10.3354/dao062133 issn: 0177-5103 e-issn: 1616-1580 Diseases of Aquatic Organisms 62(1-2): 133-145 (2004) http://hdl.handle.net/10261/253764 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100006280 |
op_rights |
none |
op_doi |
https://doi.org/10.3354/dao06213310.13039/50110000078010.13039/501100006280 |
container_title |
Diseases of Aquatic Organisms |
container_volume |
62 |
container_start_page |
133 |
op_container_end_page |
145 |
_version_ |
1790607770407403520 |