Levamisole activates several innate immune factors in Scophthalmus maximus (L.) (Teleostei)
Levamisole, originally synthesized as an anti-helminthic, has been widely used in human and veterinary medicine as an immunomodulator or adjuvant. However, data on its use in fish are scarce, and no information is available for turbot. To study the effects of levamisole treatment on the innate immun...
Published in: | International Journal of Immunopathology and Pharmacology |
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Sage Publications
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Online Access: | http://hdl.handle.net/10261/253732 https://doi.org/10.1177/039463200601900403 https://doi.org/10.13039/501100006280 |
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ftcsic:oai:digital.csic.es:10261/253732 2024-02-11T10:08:28+01:00 Levamisole activates several innate immune factors in Scophthalmus maximus (L.) (Teleostei) Álvarez-Pellitero, Mª del Pilar Sitjà-Bobadilla, Ariadna Bermúdez, Roberto Quiroga, María Isabel Ministerio de Ciencia y Tecnología (España) Stolt Sea Farm Ministerio de Educación y Ciencia (España) 2006-10 http://hdl.handle.net/10261/253732 https://doi.org/10.1177/039463200601900403 https://doi.org/10.13039/501100006280 unknown Sage Publications http://dx.doi.org/10.1177/039463200601900403 Sí doi:10.1177/039463200601900403 issn: 0394-6320 e-issn: 2058-7384 International Journal of Immunopathology and Pharmacology 19(4): 727-738 (2006) http://hdl.handle.net/10261/253732 http://dx.doi.org/10.13039/501100006280 none Fish Immune response Immunomodulation Levamisole Psetta Scophthalmus Turbot artículo http://purl.org/coar/resource_type/c_6501 2006 ftcsic https://doi.org/10.1177/03946320060190040310.13039/501100006280 2024-01-16T11:15:14Z Levamisole, originally synthesized as an anti-helminthic, has been widely used in human and veterinary medicine as an immunomodulator or adjuvant. However, data on its use in fish are scarce, and no information is available for turbot. To study the effects of levamisole treatment on the innate immune system of juvenile turbot, two different doses (D1=500 mg/kg; D2=250 mg/kg dry food) were orally administered for 2 weeks and samplings were performed at -10, 14, 28, 49 and 77 days post treatment (p.t.). Biometrical, haematological, histological and immunological data were obtained. Specific growth rate was higher in the medicated groups than in the control (C), but the difference was statistically significant only for Dl fish at day 49 p.t. The leucocytes/trombocytes ratio was significantly higher in D1 than in C fish at 14 days p.t., but decreased subsequently. At most samplings, the percentage of the circulating lymphocytes was lower and that of the progranulocytes was higher in the medicated fish than in the C ones. The percentage of fish with high haemotopoietic activity in the kidney was clearly higher in D1 and D2 fish than in C ones at some sampling points. The respiratory burst activity of blood leucocytes was significantly higher in D1 fish than in C ones in all samplings, except at day 77 p.t. when control fish experienced a rebound effect. In all medicated fish, an initial increase of such activity was observed, followed by a further decrease. Their serum peroxidases followed a contrary pattern, with a decrease in the second sampling and a subsequent and non-significant recovery, a situation also observed for serum lysozyme and complement. Therefore, oral levamisole treatment actually affects some turbot immune factors, although stimulation or depression can occur depending on the considered factor and the administered dose. These results point out the interest of further studies on the mechanisms involved in the levamisole action for its adequate use as immunomodulator. Funding for this work was ... Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) International Journal of Immunopathology and Pharmacology 19 4 727 738 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Fish Immune response Immunomodulation Levamisole Psetta Scophthalmus Turbot |
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Fish Immune response Immunomodulation Levamisole Psetta Scophthalmus Turbot Álvarez-Pellitero, Mª del Pilar Sitjà-Bobadilla, Ariadna Bermúdez, Roberto Quiroga, María Isabel Levamisole activates several innate immune factors in Scophthalmus maximus (L.) (Teleostei) |
topic_facet |
Fish Immune response Immunomodulation Levamisole Psetta Scophthalmus Turbot |
description |
Levamisole, originally synthesized as an anti-helminthic, has been widely used in human and veterinary medicine as an immunomodulator or adjuvant. However, data on its use in fish are scarce, and no information is available for turbot. To study the effects of levamisole treatment on the innate immune system of juvenile turbot, two different doses (D1=500 mg/kg; D2=250 mg/kg dry food) were orally administered for 2 weeks and samplings were performed at -10, 14, 28, 49 and 77 days post treatment (p.t.). Biometrical, haematological, histological and immunological data were obtained. Specific growth rate was higher in the medicated groups than in the control (C), but the difference was statistically significant only for Dl fish at day 49 p.t. The leucocytes/trombocytes ratio was significantly higher in D1 than in C fish at 14 days p.t., but decreased subsequently. At most samplings, the percentage of the circulating lymphocytes was lower and that of the progranulocytes was higher in the medicated fish than in the C ones. The percentage of fish with high haemotopoietic activity in the kidney was clearly higher in D1 and D2 fish than in C ones at some sampling points. The respiratory burst activity of blood leucocytes was significantly higher in D1 fish than in C ones in all samplings, except at day 77 p.t. when control fish experienced a rebound effect. In all medicated fish, an initial increase of such activity was observed, followed by a further decrease. Their serum peroxidases followed a contrary pattern, with a decrease in the second sampling and a subsequent and non-significant recovery, a situation also observed for serum lysozyme and complement. Therefore, oral levamisole treatment actually affects some turbot immune factors, although stimulation or depression can occur depending on the considered factor and the administered dose. These results point out the interest of further studies on the mechanisms involved in the levamisole action for its adequate use as immunomodulator. Funding for this work was ... |
author2 |
Ministerio de Ciencia y Tecnología (España) Stolt Sea Farm Ministerio de Educación y Ciencia (España) |
format |
Article in Journal/Newspaper |
author |
Álvarez-Pellitero, Mª del Pilar Sitjà-Bobadilla, Ariadna Bermúdez, Roberto Quiroga, María Isabel |
author_facet |
Álvarez-Pellitero, Mª del Pilar Sitjà-Bobadilla, Ariadna Bermúdez, Roberto Quiroga, María Isabel |
author_sort |
Álvarez-Pellitero, Mª del Pilar |
title |
Levamisole activates several innate immune factors in Scophthalmus maximus (L.) (Teleostei) |
title_short |
Levamisole activates several innate immune factors in Scophthalmus maximus (L.) (Teleostei) |
title_full |
Levamisole activates several innate immune factors in Scophthalmus maximus (L.) (Teleostei) |
title_fullStr |
Levamisole activates several innate immune factors in Scophthalmus maximus (L.) (Teleostei) |
title_full_unstemmed |
Levamisole activates several innate immune factors in Scophthalmus maximus (L.) (Teleostei) |
title_sort |
levamisole activates several innate immune factors in scophthalmus maximus (l.) (teleostei) |
publisher |
Sage Publications |
publishDate |
2006 |
url |
http://hdl.handle.net/10261/253732 https://doi.org/10.1177/039463200601900403 https://doi.org/10.13039/501100006280 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://dx.doi.org/10.1177/039463200601900403 Sí doi:10.1177/039463200601900403 issn: 0394-6320 e-issn: 2058-7384 International Journal of Immunopathology and Pharmacology 19(4): 727-738 (2006) http://hdl.handle.net/10261/253732 http://dx.doi.org/10.13039/501100006280 |
op_rights |
none |
op_doi |
https://doi.org/10.1177/03946320060190040310.13039/501100006280 |
container_title |
International Journal of Immunopathology and Pharmacology |
container_volume |
19 |
container_issue |
4 |
container_start_page |
727 |
op_container_end_page |
738 |
_version_ |
1790607809697546240 |