Comparison of different procedures for serotyping aquatic birnavirus
5 pages, 2 figures, 5 tables. The current classification of aquatic birnaviruses is based on seroneutralization assays with polyclonal antibodies. In this study a comparison of several procedures used for serotyping aquatic birnaviruses was made with 10 virus strains (4 reference strains from salmon...
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ftcsic:oai:digital.csic.es:10261/25363 2024-02-11T10:08:27+01:00 Comparison of different procedures for serotyping aquatic birnavirus Novoa, Beatriz Blake, S. Nicholson, B. L. Figueras Huerta, Antonio 1995-08 391208 bytes application/pdf http://hdl.handle.net/10261/25363 en eng American Society for Microbiology http://aem.asm.org/cgi/content/abstract/61/8/2925 Applied and Environmental Microbiology 61(8): 2925-2929 (1995) 0099-2240 http://hdl.handle.net/10261/25363 none artículo http://purl.org/coar/resource_type/c_6501 1995 ftcsic 2024-01-16T09:27:18Z 5 pages, 2 figures, 5 tables. The current classification of aquatic birnaviruses is based on seroneutralization assays with polyclonal antibodies. In this study a comparison of several procedures used for serotyping aquatic birnaviruses was made with 10 virus strains (4 reference strains from salmonids and 6 birnaviruses isolated from turbot [Scophthalmus maximus]). The relationships among the birnavirus strains were studied by seroneutralization assay with polyclonal antibodies and by immunodot assay with both polyclonal and monoclonal antibodies. The results were compared with a presumptive classification obtained from analysis of restriction enzyme patterns of cDNA products obtained by PCR amplification. No correlation was found among the results obtained by the different procedures. The seroneutralization and the immunodot assays with polyclonal antibodies were not useful in classifying these birnaviruses strains; however, patterns of reaction with monoclonal antibodies emphasized the individuality of the strains, particularly in the case of two strains (231 and 460) whose patterns did not correspond to established serotypes. The application of PCR and restriction enzyme analysis is a promising system for approaching the classification of this viral group on the basis of genomic differences and similarities. The variable results obtained in this comparison lead us to think that the current classification of aquatic birnavirus may not be the most accurate and there is a need for modification incorporating recent isolates, not only from salmonid species but also from marine fish. This work was supported by grant AGF93-0769-C02-02 from the Comisión Interministerial de Ciencia y Tecnología. Beatriz Novoa acknowledges the Ministerio de Educación y Ciencia (Spain) for a research fellowship. Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) |
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Digital.CSIC (Spanish National Research Council) |
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English |
description |
5 pages, 2 figures, 5 tables. The current classification of aquatic birnaviruses is based on seroneutralization assays with polyclonal antibodies. In this study a comparison of several procedures used for serotyping aquatic birnaviruses was made with 10 virus strains (4 reference strains from salmonids and 6 birnaviruses isolated from turbot [Scophthalmus maximus]). The relationships among the birnavirus strains were studied by seroneutralization assay with polyclonal antibodies and by immunodot assay with both polyclonal and monoclonal antibodies. The results were compared with a presumptive classification obtained from analysis of restriction enzyme patterns of cDNA products obtained by PCR amplification. No correlation was found among the results obtained by the different procedures. The seroneutralization and the immunodot assays with polyclonal antibodies were not useful in classifying these birnaviruses strains; however, patterns of reaction with monoclonal antibodies emphasized the individuality of the strains, particularly in the case of two strains (231 and 460) whose patterns did not correspond to established serotypes. The application of PCR and restriction enzyme analysis is a promising system for approaching the classification of this viral group on the basis of genomic differences and similarities. The variable results obtained in this comparison lead us to think that the current classification of aquatic birnavirus may not be the most accurate and there is a need for modification incorporating recent isolates, not only from salmonid species but also from marine fish. This work was supported by grant AGF93-0769-C02-02 from the Comisión Interministerial de Ciencia y Tecnología. Beatriz Novoa acknowledges the Ministerio de Educación y Ciencia (Spain) for a research fellowship. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Novoa, Beatriz Blake, S. Nicholson, B. L. Figueras Huerta, Antonio |
spellingShingle |
Novoa, Beatriz Blake, S. Nicholson, B. L. Figueras Huerta, Antonio Comparison of different procedures for serotyping aquatic birnavirus |
author_facet |
Novoa, Beatriz Blake, S. Nicholson, B. L. Figueras Huerta, Antonio |
author_sort |
Novoa, Beatriz |
title |
Comparison of different procedures for serotyping aquatic birnavirus |
title_short |
Comparison of different procedures for serotyping aquatic birnavirus |
title_full |
Comparison of different procedures for serotyping aquatic birnavirus |
title_fullStr |
Comparison of different procedures for serotyping aquatic birnavirus |
title_full_unstemmed |
Comparison of different procedures for serotyping aquatic birnavirus |
title_sort |
comparison of different procedures for serotyping aquatic birnavirus |
publisher |
American Society for Microbiology |
publishDate |
1995 |
url |
http://hdl.handle.net/10261/25363 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://aem.asm.org/cgi/content/abstract/61/8/2925 Applied and Environmental Microbiology 61(8): 2925-2929 (1995) 0099-2240 http://hdl.handle.net/10261/25363 |
op_rights |
none |
_version_ |
1790607795210420224 |