Characterization of a birnavirus isolated from diseased turbot cultured in Spain
7 pages, 5 figures, 2 tables. During 1989, light but persistent mortalities were detected in a turbot Scophthalmus maximus L. farm in Galicia (northwestern Spain) and a virus with the characteristics of a birnavirus was isolated. The purpose of this study was to characterize the viral agent and dete...
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ftcsic:oai:digital.csic.es:10261/25338 2024-02-11T10:08:27+01:00 Characterization of a birnavirus isolated from diseased turbot cultured in Spain Novoa, Beatriz Figueras Huerta, Antonio Puentes, C. F. Ledo, A. Toranzo, A. E. 1993-04-29 2140533 bytes application/pdf http://hdl.handle.net/10261/25338 en eng Inter Research http://www.int-res.com/abstracts/dao/v15/ Diseases of Aquatic Organisms 15: 163-169 (1993) 0177-5103 http://hdl.handle.net/10261/25338 open artículo http://purl.org/coar/resource_type/c_6501 1993 ftcsic 2024-01-16T09:27:18Z 7 pages, 5 figures, 2 tables. During 1989, light but persistent mortalities were detected in a turbot Scophthalmus maximus L. farm in Galicia (northwestern Spain) and a virus with the characteristics of a birnavirus was isolated. The purpose of this study was to characterize the viral agent and determine the susceptibility of turbot to this virus. Electron microscopic examination revealed that the particles were isometric, hexagonal and unenveloped with an average diameter of 58 to 60 nm. The molecular weights of the RNA segments were 1.9 and 2.0 x 10(up to 6) daltons. The cells most susceptible to the turbot isolate were the CHSE-214, FHM and RTG-2 lines and the optimal temperature range for its replication was 15 to 2OºC. The RNA and polypeptide electropherotypes show that this virus resembles the Ab serotype of infectious pancreatic necrosis virus (IPNV); however, it differs in that it replicates in the FHM cell line and is not neutralized by antisera to the classical serotypes of IPNV. Infectivity trials conducted in turbot of dlfferent sizes indicated that the virus produced mortality only in small fish (2 g), although the larger fish (30 g) harbored the virus for at least 35 d. Fish inoculated with this isolate showed no pancreatic necrosis although necrosis of the hematopoietic elements of the kidney and spleen was detected. This work was supported by Grants MAR 89-0270 from the Comision Interministenal de Ciencia y Tecnologia (CICYT), and by XUGA 70708888 from Xunta de Galicia, Spain. Beatriz Novoa acknowledges the Ministerio de Educacion y Ciencia (Spain) for a research fellowship. Peer reviewed Article in Journal/Newspaper Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) |
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Digital.CSIC (Spanish National Research Council) |
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language |
English |
description |
7 pages, 5 figures, 2 tables. During 1989, light but persistent mortalities were detected in a turbot Scophthalmus maximus L. farm in Galicia (northwestern Spain) and a virus with the characteristics of a birnavirus was isolated. The purpose of this study was to characterize the viral agent and determine the susceptibility of turbot to this virus. Electron microscopic examination revealed that the particles were isometric, hexagonal and unenveloped with an average diameter of 58 to 60 nm. The molecular weights of the RNA segments were 1.9 and 2.0 x 10(up to 6) daltons. The cells most susceptible to the turbot isolate were the CHSE-214, FHM and RTG-2 lines and the optimal temperature range for its replication was 15 to 2OºC. The RNA and polypeptide electropherotypes show that this virus resembles the Ab serotype of infectious pancreatic necrosis virus (IPNV); however, it differs in that it replicates in the FHM cell line and is not neutralized by antisera to the classical serotypes of IPNV. Infectivity trials conducted in turbot of dlfferent sizes indicated that the virus produced mortality only in small fish (2 g), although the larger fish (30 g) harbored the virus for at least 35 d. Fish inoculated with this isolate showed no pancreatic necrosis although necrosis of the hematopoietic elements of the kidney and spleen was detected. This work was supported by Grants MAR 89-0270 from the Comision Interministenal de Ciencia y Tecnologia (CICYT), and by XUGA 70708888 from Xunta de Galicia, Spain. Beatriz Novoa acknowledges the Ministerio de Educacion y Ciencia (Spain) for a research fellowship. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Novoa, Beatriz Figueras Huerta, Antonio Puentes, C. F. Ledo, A. Toranzo, A. E. |
spellingShingle |
Novoa, Beatriz Figueras Huerta, Antonio Puentes, C. F. Ledo, A. Toranzo, A. E. Characterization of a birnavirus isolated from diseased turbot cultured in Spain |
author_facet |
Novoa, Beatriz Figueras Huerta, Antonio Puentes, C. F. Ledo, A. Toranzo, A. E. |
author_sort |
Novoa, Beatriz |
title |
Characterization of a birnavirus isolated from diseased turbot cultured in Spain |
title_short |
Characterization of a birnavirus isolated from diseased turbot cultured in Spain |
title_full |
Characterization of a birnavirus isolated from diseased turbot cultured in Spain |
title_fullStr |
Characterization of a birnavirus isolated from diseased turbot cultured in Spain |
title_full_unstemmed |
Characterization of a birnavirus isolated from diseased turbot cultured in Spain |
title_sort |
characterization of a birnavirus isolated from diseased turbot cultured in spain |
publisher |
Inter Research |
publishDate |
1993 |
url |
http://hdl.handle.net/10261/25338 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
http://www.int-res.com/abstracts/dao/v15/ Diseases of Aquatic Organisms 15: 163-169 (1993) 0177-5103 http://hdl.handle.net/10261/25338 |
op_rights |
open |
_version_ |
1790607794439716864 |