Sex identification of wolf (Canis lupus) using non-invasive samples
We have developed new specific primers for sex determination from forensic samples of wolves (Canis lupus), such as hair, saliva, faecal, tooth and urine samples. In order to improve molecular sexing, we performed a multiplex semi-nested polymerase chain reaction (PCR) and several replicated amplifi...
Published in: | Conservation Genetics |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Nature
2009
|
Subjects: | |
Online Access: | http://hdl.handle.net/10261/250047 https://doi.org/10.1007/s10592-008-9565-6 |
id |
ftcsic:oai:digital.csic.es:10261/250047 |
---|---|
record_format |
openpolar |
spelling |
ftcsic:oai:digital.csic.es:10261/250047 2024-02-11T10:02:42+01:00 Sex identification of wolf (Canis lupus) using non-invasive samples Sastre, Natalia Francino, Olga Lampreave, Gabriel Bologov, Vladimir V. López-Martín, José María Sánchez, Armand Ramírez, Óscar 2009 http://hdl.handle.net/10261/250047 https://doi.org/10.1007/s10592-008-9565-6 en eng Springer Nature https://doi.org/10.1007/s10592-008-9565-6 No Conservation Genetics 10: 555–558 (2009) 1566-0621 http://hdl.handle.net/10261/250047 doi:10.1007/s10592-008-9565-6 none artículo http://purl.org/coar/resource_type/c_6501 2009 ftcsic https://doi.org/10.1007/s10592-008-9565-6 2024-01-16T11:13:20Z We have developed new specific primers for sex determination from forensic samples of wolves (Canis lupus), such as hair, saliva, faecal, tooth and urine samples. In order to improve molecular sexing, we performed a multiplex semi-nested polymerase chain reaction (PCR) and several replicated amplifications per sample to avoid errors in low quantity DNA samples, such as allelic dropout and false alleles. The sex of individuals is automatically determined by capillary electrophoresis with a fluorescently labelled internal sex-specific primer from each pair. Our method yielded sex identification on 100% of invasive samples and 93% of forensic samples, being one of the highest success rates obtained from wild animals. Peer reviewed Article in Journal/Newspaper Canis lupus Digital.CSIC (Spanish National Research Council) Conservation Genetics 10 3 555 558 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
We have developed new specific primers for sex determination from forensic samples of wolves (Canis lupus), such as hair, saliva, faecal, tooth and urine samples. In order to improve molecular sexing, we performed a multiplex semi-nested polymerase chain reaction (PCR) and several replicated amplifications per sample to avoid errors in low quantity DNA samples, such as allelic dropout and false alleles. The sex of individuals is automatically determined by capillary electrophoresis with a fluorescently labelled internal sex-specific primer from each pair. Our method yielded sex identification on 100% of invasive samples and 93% of forensic samples, being one of the highest success rates obtained from wild animals. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Sastre, Natalia Francino, Olga Lampreave, Gabriel Bologov, Vladimir V. López-Martín, José María Sánchez, Armand Ramírez, Óscar |
spellingShingle |
Sastre, Natalia Francino, Olga Lampreave, Gabriel Bologov, Vladimir V. López-Martín, José María Sánchez, Armand Ramírez, Óscar Sex identification of wolf (Canis lupus) using non-invasive samples |
author_facet |
Sastre, Natalia Francino, Olga Lampreave, Gabriel Bologov, Vladimir V. López-Martín, José María Sánchez, Armand Ramírez, Óscar |
author_sort |
Sastre, Natalia |
title |
Sex identification of wolf (Canis lupus) using non-invasive samples |
title_short |
Sex identification of wolf (Canis lupus) using non-invasive samples |
title_full |
Sex identification of wolf (Canis lupus) using non-invasive samples |
title_fullStr |
Sex identification of wolf (Canis lupus) using non-invasive samples |
title_full_unstemmed |
Sex identification of wolf (Canis lupus) using non-invasive samples |
title_sort |
sex identification of wolf (canis lupus) using non-invasive samples |
publisher |
Springer Nature |
publishDate |
2009 |
url |
http://hdl.handle.net/10261/250047 https://doi.org/10.1007/s10592-008-9565-6 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
https://doi.org/10.1007/s10592-008-9565-6 No Conservation Genetics 10: 555–558 (2009) 1566-0621 http://hdl.handle.net/10261/250047 doi:10.1007/s10592-008-9565-6 |
op_rights |
none |
op_doi |
https://doi.org/10.1007/s10592-008-9565-6 |
container_title |
Conservation Genetics |
container_volume |
10 |
container_issue |
3 |
container_start_page |
555 |
op_container_end_page |
558 |
_version_ |
1790598753036533760 |