Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins
The recently cloned rice transglutaminase gene (tgo) is the second plant transglutaminase identified to date (Campos et al. Plant Sci. 205–206 (2013) 97–110). Similarly to its counterpart in maize (tgz), this rice TGase was localized in the chloroplast, although in this case not exclusively. To furt...
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Online Access: | http://hdl.handle.net/10261/249762 https://doi.org/10.1016/j.plantsci.2014.09.004 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100002809 https://doi.org/10.13039/501100003086 https://doi.org/10.13039/501100004892 |
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ftcsic:oai:digital.csic.es:10261/249762 2024-02-11T10:08:23+01:00 Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins Campos, Narciso Torné, Josep María Bleda, María José Manich, Albert M. Urreta, I. Montalbán, I. A. Castañón, S. Moncalean, P. Santos, Mireya Ministerio de Economía y Competitividad (España) Eusko Jaurlaritza Agencia Española de Cooperación Internacional para el Desarrollo Ministerio de Ciencia e Innovación (España) Generalitat de Catalunya 2014 http://hdl.handle.net/10261/249762 https://doi.org/10.1016/j.plantsci.2014.09.004 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100002809 https://doi.org/10.13039/501100003086 https://doi.org/10.13039/501100004892 en eng Elsevier https://doi.org/10.1016/j.plantsci.2014.09.004 Sí Plant Science 229: 142-153 (2014) 0168-9452 http://hdl.handle.net/10261/249762 doi:10.1016/j.plantsci.2014.09.004 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003086 http://dx.doi.org/10.13039/501100004892 none artículo http://purl.org/coar/resource_type/c_6501 2014 ftcsic https://doi.org/10.1016/j.plantsci.2014.09.00410.13039/50110000483710.13039/50110000332910.13039/50110000280910.13039/50110000308610.13039/501100004892 2024-01-16T11:13:10Z The recently cloned rice transglutaminase gene (tgo) is the second plant transglutaminase identified to date (Campos et al. Plant Sci. 205–206 (2013) 97–110). Similarly to its counterpart in maize (tgz), this rice TGase was localized in the chloroplast, although in this case not exclusively. To further characterise plastidial tgo functionality, proteomic and transcriptomic studies were carried out to identify possible TGO-related proteins. Some LHCII antenna proteins were identified as TGO related using an in vitro proteomic approach, as well as ATPase and some PSII core proteins by mass spectrometry. To study the relationship between TGO and other plastidial proteins, a transcriptomic in vivo Dynamic Array (Fluidigm™) was used to analyse the mRNA expression of 30 plastidial genes with respect to that of tgo, in rice plants subjected to different periods of continuous illumination. The results indicated a gene-dependent tendency in the expression pattern that was related to tgo expression and to the illumination cycle. For certain genes, including tgo, significant differences between treatments, principally at the initiation and/or at the end of the illumination period, connected with the day/night cycling of gene expression, were observed. The tgo expression was especially related to plastidial proteins involved in photoprotection and the thylakoid electrochemical gradient. This study was supported by the Spanish projects MEC BFU2006-15115-01/BMC, BFU2009-08575 and a collaborative project (TRANSBIO 2011) with the Biotechnology Department of Neiker Technalia, financed by the Environmental, Territorial Planification, Agriculture and Fish Dept., basque Gov, Spain. N. Campos had a pre-doctoral fellowship from the Agencia Española de Cooperación Internacional para el Desarrollo (AECID). The authors would especially like to thank Oriol Casagran (CRAG Genomic Services), Sami Irar (CRAG Proteomic Services), and Shirley Burgess (English correction). The authors would also like to thank the Spanish Ministry of Science ... Article in Journal/Newspaper sami Digital.CSIC (Spanish National Research Council) Burgess ENVELOPE(76.128,76.128,-69.415,-69.415) Española ENVELOPE(-60.383,-60.383,-62.660,-62.660) Plant Science 229 142 153 |
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Open Polar |
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Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
The recently cloned rice transglutaminase gene (tgo) is the second plant transglutaminase identified to date (Campos et al. Plant Sci. 205–206 (2013) 97–110). Similarly to its counterpart in maize (tgz), this rice TGase was localized in the chloroplast, although in this case not exclusively. To further characterise plastidial tgo functionality, proteomic and transcriptomic studies were carried out to identify possible TGO-related proteins. Some LHCII antenna proteins were identified as TGO related using an in vitro proteomic approach, as well as ATPase and some PSII core proteins by mass spectrometry. To study the relationship between TGO and other plastidial proteins, a transcriptomic in vivo Dynamic Array (Fluidigm™) was used to analyse the mRNA expression of 30 plastidial genes with respect to that of tgo, in rice plants subjected to different periods of continuous illumination. The results indicated a gene-dependent tendency in the expression pattern that was related to tgo expression and to the illumination cycle. For certain genes, including tgo, significant differences between treatments, principally at the initiation and/or at the end of the illumination period, connected with the day/night cycling of gene expression, were observed. The tgo expression was especially related to plastidial proteins involved in photoprotection and the thylakoid electrochemical gradient. This study was supported by the Spanish projects MEC BFU2006-15115-01/BMC, BFU2009-08575 and a collaborative project (TRANSBIO 2011) with the Biotechnology Department of Neiker Technalia, financed by the Environmental, Territorial Planification, Agriculture and Fish Dept., basque Gov, Spain. N. Campos had a pre-doctoral fellowship from the Agencia Española de Cooperación Internacional para el Desarrollo (AECID). The authors would especially like to thank Oriol Casagran (CRAG Genomic Services), Sami Irar (CRAG Proteomic Services), and Shirley Burgess (English correction). The authors would also like to thank the Spanish Ministry of Science ... |
author2 |
Ministerio de Economía y Competitividad (España) Eusko Jaurlaritza Agencia Española de Cooperación Internacional para el Desarrollo Ministerio de Ciencia e Innovación (España) Generalitat de Catalunya |
format |
Article in Journal/Newspaper |
author |
Campos, Narciso Torné, Josep María Bleda, María José Manich, Albert M. Urreta, I. Montalbán, I. A. Castañón, S. Moncalean, P. Santos, Mireya |
spellingShingle |
Campos, Narciso Torné, Josep María Bleda, María José Manich, Albert M. Urreta, I. Montalbán, I. A. Castañón, S. Moncalean, P. Santos, Mireya Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins |
author_facet |
Campos, Narciso Torné, Josep María Bleda, María José Manich, Albert M. Urreta, I. Montalbán, I. A. Castañón, S. Moncalean, P. Santos, Mireya |
author_sort |
Campos, Narciso |
title |
Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins |
title_short |
Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins |
title_full |
Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins |
title_fullStr |
Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins |
title_full_unstemmed |
Proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins |
title_sort |
proteomic and transcriptomic analysis of rice tranglutaminase and chloroplast-related proteins |
publisher |
Elsevier |
publishDate |
2014 |
url |
http://hdl.handle.net/10261/249762 https://doi.org/10.1016/j.plantsci.2014.09.004 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100003329 https://doi.org/10.13039/501100002809 https://doi.org/10.13039/501100003086 https://doi.org/10.13039/501100004892 |
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ENVELOPE(76.128,76.128,-69.415,-69.415) ENVELOPE(-60.383,-60.383,-62.660,-62.660) |
geographic |
Burgess Española |
geographic_facet |
Burgess Española |
genre |
sami |
genre_facet |
sami |
op_relation |
https://doi.org/10.1016/j.plantsci.2014.09.004 Sí Plant Science 229: 142-153 (2014) 0168-9452 http://hdl.handle.net/10261/249762 doi:10.1016/j.plantsci.2014.09.004 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002809 http://dx.doi.org/10.13039/501100003086 http://dx.doi.org/10.13039/501100004892 |
op_rights |
none |
op_doi |
https://doi.org/10.1016/j.plantsci.2014.09.00410.13039/50110000483710.13039/50110000332910.13039/50110000280910.13039/50110000308610.13039/501100004892 |
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Plant Science |
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229 |
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142 |
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153 |
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1790607610845593600 |