Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica
11 pages, 6 figures, 4 tables A previous report of high levels of members of the domainArchaea in Antarctic coastal waters prompted us to investigate the ecology of Antarctic planktonic prokaryotes. rRNA hybridization techniques and denaturing gradient gel electrophoresis (DGGE) analysis of the bact...
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1998
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Online Access: | http://hdl.handle.net/10261/243294 https://doi.org/10.1128/AEM.64.7.2585-2595.1998 |
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ftcsic:oai:digital.csic.es:10261/243294 2024-02-11T09:56:31+01:00 Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica Murray, Alison Preston, C.M. Massana, Ramon Taylor, Lance T. Blakis, A. Wu, Key Y. DeLong, Edward F. 1998-07 http://hdl.handle.net/10261/243294 https://doi.org/10.1128/AEM.64.7.2585-2595.1998 en eng American Society for Microbiology https://doi.org/10.1128/AEM.64.7.2585-2595.1998 No Applied and Environmental Microbiology 64(7): 2585-2595 (1998) 0099-2240 http://hdl.handle.net/10261/243294 doi:10.1128/AEM.64.7.2585-2595.1998 1098-5336 none artículo http://purl.org/coar/resource_type/c_6501 1998 ftcsic https://doi.org/10.1128/AEM.64.7.2585-2595.1998 2024-01-16T11:09:49Z 11 pages, 6 figures, 4 tables A previous report of high levels of members of the domainArchaea in Antarctic coastal waters prompted us to investigate the ecology of Antarctic planktonic prokaryotes. rRNA hybridization techniques and denaturing gradient gel electrophoresis (DGGE) analysis of the bacterial V3 region were used to study variation in Antarctic picoplankton assemblages. In Anvers Island nearshore waters during late winter to early spring, the amounts of archaeal rRNA ranged from 17.1 to 3.6% of the total picoplankton rRNA in 1996 and from 16.0 to 1.0% of the total rRNA in 1995. Offshore in the Palmer Basin, the levels of archaeal rRNA throughout the water column were higher (average, 24% of the total rRNA) during the same period in 1996. The archaeal rRNA levels in nearshore waters followed a highly seasonal pattern and markedly decreased during the austral summer at two stations. There was a significant negative correlation between archaeal rRNA levels and phytoplankton levels (as inferred from chlorophyll a concentrations) in nearshore surface waters during the early spring of 1995 and during an 8-month period in 1996 and 1997. In situ hybridization experiments revealed that 5 to 14% of DAPI (4′,6-diamidino-2-phenylindole)-stained cells were archaeal, corresponding to 0.9 × 104 to 2.7 × 104 archaeal cells per ml, in late winter 1996 samples. Analysis of bacterial ribosomal DNA fragments by DGGE revealed that the assemblage composition may reflect changes in water column stability, depth, or season. The data indicate that changes in Antarctic seasons are accompanied by significant shifts in the species composition of bacterioplankton assemblages and by large decreases in the relative proportion of archaeal rRNA in the nearshore water column Peer reviewed Article in Journal/Newspaper Antarc* Antarctic Antarctica Anvers Island Digital.CSIC (Spanish National Research Council) Antarctic Anvers ENVELOPE(-63.500,-63.500,-64.600,-64.600) Anvers Island ENVELOPE(-63.500,-63.500,-64.600,-64.600) Austral Palmer Basin ENVELOPE(-64.400,-64.400,-64.950,-64.950) Applied and Environmental Microbiology 64 7 2585 2595 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
description |
11 pages, 6 figures, 4 tables A previous report of high levels of members of the domainArchaea in Antarctic coastal waters prompted us to investigate the ecology of Antarctic planktonic prokaryotes. rRNA hybridization techniques and denaturing gradient gel electrophoresis (DGGE) analysis of the bacterial V3 region were used to study variation in Antarctic picoplankton assemblages. In Anvers Island nearshore waters during late winter to early spring, the amounts of archaeal rRNA ranged from 17.1 to 3.6% of the total picoplankton rRNA in 1996 and from 16.0 to 1.0% of the total rRNA in 1995. Offshore in the Palmer Basin, the levels of archaeal rRNA throughout the water column were higher (average, 24% of the total rRNA) during the same period in 1996. The archaeal rRNA levels in nearshore waters followed a highly seasonal pattern and markedly decreased during the austral summer at two stations. There was a significant negative correlation between archaeal rRNA levels and phytoplankton levels (as inferred from chlorophyll a concentrations) in nearshore surface waters during the early spring of 1995 and during an 8-month period in 1996 and 1997. In situ hybridization experiments revealed that 5 to 14% of DAPI (4′,6-diamidino-2-phenylindole)-stained cells were archaeal, corresponding to 0.9 × 104 to 2.7 × 104 archaeal cells per ml, in late winter 1996 samples. Analysis of bacterial ribosomal DNA fragments by DGGE revealed that the assemblage composition may reflect changes in water column stability, depth, or season. The data indicate that changes in Antarctic seasons are accompanied by significant shifts in the species composition of bacterioplankton assemblages and by large decreases in the relative proportion of archaeal rRNA in the nearshore water column Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Murray, Alison Preston, C.M. Massana, Ramon Taylor, Lance T. Blakis, A. Wu, Key Y. DeLong, Edward F. |
spellingShingle |
Murray, Alison Preston, C.M. Massana, Ramon Taylor, Lance T. Blakis, A. Wu, Key Y. DeLong, Edward F. Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica |
author_facet |
Murray, Alison Preston, C.M. Massana, Ramon Taylor, Lance T. Blakis, A. Wu, Key Y. DeLong, Edward F. |
author_sort |
Murray, Alison |
title |
Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica |
title_short |
Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica |
title_full |
Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica |
title_fullStr |
Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica |
title_full_unstemmed |
Seasonal and Spatial Variability of Bacterial and Archaeal Assemblages in the Coastal Waters near Anvers Island, Antarctica |
title_sort |
seasonal and spatial variability of bacterial and archaeal assemblages in the coastal waters near anvers island, antarctica |
publisher |
American Society for Microbiology |
publishDate |
1998 |
url |
http://hdl.handle.net/10261/243294 https://doi.org/10.1128/AEM.64.7.2585-2595.1998 |
long_lat |
ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(-64.400,-64.400,-64.950,-64.950) |
geographic |
Antarctic Anvers Anvers Island Austral Palmer Basin |
geographic_facet |
Antarctic Anvers Anvers Island Austral Palmer Basin |
genre |
Antarc* Antarctic Antarctica Anvers Island |
genre_facet |
Antarc* Antarctic Antarctica Anvers Island |
op_relation |
https://doi.org/10.1128/AEM.64.7.2585-2595.1998 No Applied and Environmental Microbiology 64(7): 2585-2595 (1998) 0099-2240 http://hdl.handle.net/10261/243294 doi:10.1128/AEM.64.7.2585-2595.1998 1098-5336 |
op_rights |
none |
op_doi |
https://doi.org/10.1128/AEM.64.7.2585-2595.1998 |
container_title |
Applied and Environmental Microbiology |
container_volume |
64 |
container_issue |
7 |
container_start_page |
2585 |
op_container_end_page |
2595 |
_version_ |
1790603745144340480 |