Immobilization of Phaeobacter 27-4 in biofilters as a strategy for the control of Vibrionaceae infections in marine fish larval rearing

• Main Author: Prol García, María Jesús
• Other Authors: Pintado, José, Planas, Miguel, Santos, Ysabel
• Format: Doctoral or Postdoctoral Thesis
• Language: Galician; English
• Published: 2010
• Subjects: Biofilters; Larval rearing; Probiotics; Bacterial infections; PCR; Hatcheries; Turbot; Rotifer
• Online Access: http://hdl.handle.net/10261/24283

The purpose of this Ph.D. Thesis was to study the immobilization of the probiotic strain Phaeobacter 27-4 in biofilters as a new strategy for the control of Vibrionaceae infections in turbot (Psetta maxima) larval rearing. This new strategy guarantees the permanence of the probiotic bacteria in the rearing system and protects turbot larvae against the fish pathogen Listonella anguillarum 90-11-287 at the beginning of exogenous feeding on the rotifer Brachionus plicatilis. As a first step to accomplish the objectives, a reproducible and specific real-time PCR method was developed for detection and quantification of the fish probiotic Phaeobacter 27-4 and the target pathogens L. anguillarum 90-11-287 and V. splendidus DMC-1 in presence of all the organisms involved in turbot larval rearing: microalgae (Isochrysis galbana), rotifers (Brachionus plicatilis), Artemia nauplii and turbot larvae.The developed real-time PCR protocol allowed monitoring and quantification of probiotic and pathogenic bacteria during in vivo trials.The developed real-time PCR protocol allowed monitoring and quantification of probiotic and pathogenic bacteria during in vivo trials.The developed real-time PCR protocol allowed monitoring and quantification of probiotic and pathogenic bacteria during in vivo trials.The developed real-time PCR protocol allowed monitoring and quantification of probiotic and pathogenic bacteria during in vivo trials. The developed real-time PCR protocol allowed monitoring and quantification of probiotic and pathogenic bacteria during in vivo trials. As rotifer is the usual way of entry for potential pathogenic Vibrionaceae into fish larvae, in a second step in this Ph.D. Thesis, the colonization and residence time of two pathogenic Vibrionaceae strains (L. anguillarum 90-11-287 and V. splendidus DMC-1) in rotifers were studied. L. anguillarum colonized rotifers more efficiently than V. splendidus and both pathogenic strains were released from rotifers to seawater, after infected rotifers were transferred to ...