Specifc chemical incorporation of l‑DOPA and functionalized l‑DOPA‑hyaluronic acid in Candida antarctica lipase: creating potential mussel‑inspired bioadhesives
12 pags., 8 figs., 4 tabs. Mussel adhesives proteins have been extensively studied as a promising alternative in bioadhesives due to their ability to provide durable anchoring under diferent surfaces in seawater. These charasteristics have been attributed to the presence of the reduced catechol form...
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Springer Nature
2020
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Online Access: | http://hdl.handle.net/10261/236368 https://doi.org/10.1007/s42452-020-03545-w https://doi.org/10.13039/501100004587 https://doi.org/10.13039/501100007041 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003339 |
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ftcsic:oai:digital.csic.es:10261/236368 2024-02-11T09:57:57+01:00 Specifc chemical incorporation of l‑DOPA and functionalized l‑DOPA‑hyaluronic acid in Candida antarctica lipase: creating potential mussel‑inspired bioadhesives Jaramillo, Johana Rodríguez-Oliva, Ivan Abian, Olga Palomo, Jose M. Consejo Superior de Investigaciones Científicas (España) Instituto de Salud Carlos III European Commission Diputación General de Aragón Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España) Universidad de Zaragoza 2020-09-24 http://hdl.handle.net/10261/236368 https://doi.org/10.1007/s42452-020-03545-w https://doi.org/10.13039/501100004587 https://doi.org/10.13039/501100007041 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003339 en eng Springer Nature Postprint https://doi.org/10.1007/s42452-020-03545-w Sí SN Applied Sciences 2:1731 (2020) http://hdl.handle.net/10261/236368 doi:10.1007/s42452-020-03545-w 2523-3971 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100007041 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 none Mussel-inspired bioadhesives DOPA DOPA-polymers Chemical modification Hyaluronic acid Lipase artículo http://purl.org/coar/resource_type/c_6501 2020 ftcsic https://doi.org/10.1007/s42452-020-03545-w10.13039/50110000458710.13039/50110000704110.13039/50110000078010.13039/501100003339 2024-01-16T11:06:11Z 12 pags., 8 figs., 4 tabs. Mussel adhesives proteins have been extensively studied as a promising alternative in bioadhesives due to their ability to provide durable anchoring under diferent surfaces in seawater. These charasteristics have been attributed to the presence of the reduced catechol form, 3,4-dihydroxyphenylalanine (DOPA) of its structure. However, its practical applications have been limited due to drawbacks with natural extraction. Here, a novel method have been described for site-specifc chemical incorporation of l-3,4-dihydroxyphenylalanine methyl ester (l-DOPAME) into proteins, in particular Candida antartica fraction B (CAL-B) lipase. Two strategies were followed, direct conjugation of DOPA at the C-terminus on the surface of the protein, and protein conjugation with tailor-made glycopolymers (DOPA-hyaluronic acid (HA) polymers) at the N-terminus. In all cases, the characterization of the new DOPA-proteins was carried out using circular dichroism, fuorescence or mass spectrometry. An improvement in the activity (in some cases more than 2 times) or the thermostability of CAL-B (with a half live 4 fold greater in some cases) was found by the incorporation of DOPA molecules. These DOPA-proteins showed excellent underwater covalent adhesive ability on amino functionalized surfaces in aqueous media compared to other modifed [e.g. tyrosine modifed (TYR)] CAL-B proteins. At pH 8.5, CALB-DOPA proteins were completely adsorbed after 90 min of incubation, whereas about 10% of CALB-HA or CALB-TYR proteins were adsorbed at the same time. Native CAL-B adsorption was not observed. These results suggest a potential application of these DOPA-proteins as bioglues or bioadhesives for practical underwater applications. s This work was supported by the Spanish National Research Council (CSIC), Fondo de Investigaciones Sanitarias from Instituto de Salud Carlos III, and European Union (ERDF/ ESF, ‘Investing in your future’) (PI15/00663 and PI18/00349 to OA); Diputación General de Aragón (Translation Research ... Article in Journal/Newspaper Antarc* Antarctica antartic* Digital.CSIC (Spanish National Research Council) SN Applied Sciences 2 10 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Mussel-inspired bioadhesives DOPA DOPA-polymers Chemical modification Hyaluronic acid Lipase |
spellingShingle |
Mussel-inspired bioadhesives DOPA DOPA-polymers Chemical modification Hyaluronic acid Lipase Jaramillo, Johana Rodríguez-Oliva, Ivan Abian, Olga Palomo, Jose M. Specifc chemical incorporation of l‑DOPA and functionalized l‑DOPA‑hyaluronic acid in Candida antarctica lipase: creating potential mussel‑inspired bioadhesives |
topic_facet |
Mussel-inspired bioadhesives DOPA DOPA-polymers Chemical modification Hyaluronic acid Lipase |
description |
12 pags., 8 figs., 4 tabs. Mussel adhesives proteins have been extensively studied as a promising alternative in bioadhesives due to their ability to provide durable anchoring under diferent surfaces in seawater. These charasteristics have been attributed to the presence of the reduced catechol form, 3,4-dihydroxyphenylalanine (DOPA) of its structure. However, its practical applications have been limited due to drawbacks with natural extraction. Here, a novel method have been described for site-specifc chemical incorporation of l-3,4-dihydroxyphenylalanine methyl ester (l-DOPAME) into proteins, in particular Candida antartica fraction B (CAL-B) lipase. Two strategies were followed, direct conjugation of DOPA at the C-terminus on the surface of the protein, and protein conjugation with tailor-made glycopolymers (DOPA-hyaluronic acid (HA) polymers) at the N-terminus. In all cases, the characterization of the new DOPA-proteins was carried out using circular dichroism, fuorescence or mass spectrometry. An improvement in the activity (in some cases more than 2 times) or the thermostability of CAL-B (with a half live 4 fold greater in some cases) was found by the incorporation of DOPA molecules. These DOPA-proteins showed excellent underwater covalent adhesive ability on amino functionalized surfaces in aqueous media compared to other modifed [e.g. tyrosine modifed (TYR)] CAL-B proteins. At pH 8.5, CALB-DOPA proteins were completely adsorbed after 90 min of incubation, whereas about 10% of CALB-HA or CALB-TYR proteins were adsorbed at the same time. Native CAL-B adsorption was not observed. These results suggest a potential application of these DOPA-proteins as bioglues or bioadhesives for practical underwater applications. s This work was supported by the Spanish National Research Council (CSIC), Fondo de Investigaciones Sanitarias from Instituto de Salud Carlos III, and European Union (ERDF/ ESF, ‘Investing in your future’) (PI15/00663 and PI18/00349 to OA); Diputación General de Aragón (Translation Research ... |
author2 |
Consejo Superior de Investigaciones Científicas (España) Instituto de Salud Carlos III European Commission Diputación General de Aragón Centro de Investigación Biomédica en Red Enfermedades Hepáticas y Digestivas (España) Universidad de Zaragoza |
format |
Article in Journal/Newspaper |
author |
Jaramillo, Johana Rodríguez-Oliva, Ivan Abian, Olga Palomo, Jose M. |
author_facet |
Jaramillo, Johana Rodríguez-Oliva, Ivan Abian, Olga Palomo, Jose M. |
author_sort |
Jaramillo, Johana |
title |
Specifc chemical incorporation of l‑DOPA and functionalized l‑DOPA‑hyaluronic acid in Candida antarctica lipase: creating potential mussel‑inspired bioadhesives |
title_short |
Specifc chemical incorporation of l‑DOPA and functionalized l‑DOPA‑hyaluronic acid in Candida antarctica lipase: creating potential mussel‑inspired bioadhesives |
title_full |
Specifc chemical incorporation of l‑DOPA and functionalized l‑DOPA‑hyaluronic acid in Candida antarctica lipase: creating potential mussel‑inspired bioadhesives |
title_fullStr |
Specifc chemical incorporation of l‑DOPA and functionalized l‑DOPA‑hyaluronic acid in Candida antarctica lipase: creating potential mussel‑inspired bioadhesives |
title_full_unstemmed |
Specifc chemical incorporation of l‑DOPA and functionalized l‑DOPA‑hyaluronic acid in Candida antarctica lipase: creating potential mussel‑inspired bioadhesives |
title_sort |
specifc chemical incorporation of l‑dopa and functionalized l‑dopa‑hyaluronic acid in candida antarctica lipase: creating potential mussel‑inspired bioadhesives |
publisher |
Springer Nature |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/236368 https://doi.org/10.1007/s42452-020-03545-w https://doi.org/10.13039/501100004587 https://doi.org/10.13039/501100007041 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003339 |
genre |
Antarc* Antarctica antartic* |
genre_facet |
Antarc* Antarctica antartic* |
op_relation |
Postprint https://doi.org/10.1007/s42452-020-03545-w Sí SN Applied Sciences 2:1731 (2020) http://hdl.handle.net/10261/236368 doi:10.1007/s42452-020-03545-w 2523-3971 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100007041 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 |
op_rights |
none |
op_doi |
https://doi.org/10.1007/s42452-020-03545-w10.13039/50110000458710.13039/50110000704110.13039/50110000078010.13039/501100003339 |
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SN Applied Sciences |
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2 |
container_issue |
10 |
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1790593488524410880 |