Metabolt: An in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures
Metabolt is a portable soil incubator to characterize the metabolic activity of microbial ecosystems in soils. It measures the electrical conductivity, the redox potential, and the concentration of certain metabolism-related gases in the headspace just above a given sample of regolith. In its curren...
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Molecular Diversity Preservation International
2020
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ftcsic:oai:digital.csic.es:10261/234090 2024-02-11T10:01:44+01:00 Metabolt: An in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures Nazarious, Miracle Israel Zorzano, María Paz Martín-Torres, F. J. 2020 http://hdl.handle.net/10261/234090 unknown Molecular Diversity Preservation International http://dx.doi.org/10.3390/s20164479 Sí issn: 1424-8220 Sensors 20: 1- 18 (2020) http://hdl.handle.net/10261/234090 open artículo http://purl.org/coar/resource_type/c_6501 2020 ftcsic https://doi.org/10.3390/s20164479 2024-01-16T11:04:41Z Metabolt is a portable soil incubator to characterize the metabolic activity of microbial ecosystems in soils. It measures the electrical conductivity, the redox potential, and the concentration of certain metabolism-related gases in the headspace just above a given sample of regolith. In its current design, the overall weight of Metabolt, including the soils (250 g), is 1.9 kg with a maximum power consumption of 1.5 W. Metabolt has been designed to monitor the activity of the soil microbiome for Earth and space applications. In particular, it can be used to monitor the health of soils, the atmospheric-regolith fixation, and release of gaseous species such as N2, H2O, CO2, O2, N2O, NH3, etc., that affect the Earth climate and atmospheric chemistry. It may be used to detect and monitor life signatures in soils, treated or untreated, as well as in controlled environments like greenhouse facilities in space, laboratory research environments like anaerobic chambers, or simulating facilities with different atmospheres and pressures. To illustrate its operation, we tested the instrument with sub-arctic soil samples at Earth environmental conditions under three different conditions: (i) no treatment (unperturbed); (ii) sterilized soil: after heating at 125¿C for 35.4 h (thermal stress); (iii) stressed soil: after adding 25% CaCl2 brine (osmotic stress); with and without addition of 0.5% glucose solution (for control). All the samples showed some distinguishable metabolic response, however there was a time delay on its appearance which depends on the treatment applied to the samples: 80 h for thermal stress without glucose, 59 h with glucose; 36 h for osmotic stress with glucose and no significant reactivation in the pure water case. This instrument shows that, over time, there is a clear observable footprint of the electrochemical signatures in the redox profile which is complementary to the gaseous footprint of the metabolic activity through respiration With funding from the Spanish government through the "María de ... Article in Journal/Newspaper Arctic Digital.CSIC (Spanish National Research Council) Arctic Sensors 20 16 4479 |
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Digital.CSIC (Spanish National Research Council) |
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unknown |
description |
Metabolt is a portable soil incubator to characterize the metabolic activity of microbial ecosystems in soils. It measures the electrical conductivity, the redox potential, and the concentration of certain metabolism-related gases in the headspace just above a given sample of regolith. In its current design, the overall weight of Metabolt, including the soils (250 g), is 1.9 kg with a maximum power consumption of 1.5 W. Metabolt has been designed to monitor the activity of the soil microbiome for Earth and space applications. In particular, it can be used to monitor the health of soils, the atmospheric-regolith fixation, and release of gaseous species such as N2, H2O, CO2, O2, N2O, NH3, etc., that affect the Earth climate and atmospheric chemistry. It may be used to detect and monitor life signatures in soils, treated or untreated, as well as in controlled environments like greenhouse facilities in space, laboratory research environments like anaerobic chambers, or simulating facilities with different atmospheres and pressures. To illustrate its operation, we tested the instrument with sub-arctic soil samples at Earth environmental conditions under three different conditions: (i) no treatment (unperturbed); (ii) sterilized soil: after heating at 125¿C for 35.4 h (thermal stress); (iii) stressed soil: after adding 25% CaCl2 brine (osmotic stress); with and without addition of 0.5% glucose solution (for control). All the samples showed some distinguishable metabolic response, however there was a time delay on its appearance which depends on the treatment applied to the samples: 80 h for thermal stress without glucose, 59 h with glucose; 36 h for osmotic stress with glucose and no significant reactivation in the pure water case. This instrument shows that, over time, there is a clear observable footprint of the electrochemical signatures in the redox profile which is complementary to the gaseous footprint of the metabolic activity through respiration With funding from the Spanish government through the "María de ... |
format |
Article in Journal/Newspaper |
author |
Nazarious, Miracle Israel Zorzano, María Paz Martín-Torres, F. J. |
spellingShingle |
Nazarious, Miracle Israel Zorzano, María Paz Martín-Torres, F. J. Metabolt: An in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures |
author_facet |
Nazarious, Miracle Israel Zorzano, María Paz Martín-Torres, F. J. |
author_sort |
Nazarious, Miracle Israel |
title |
Metabolt: An in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures |
title_short |
Metabolt: An in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures |
title_full |
Metabolt: An in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures |
title_fullStr |
Metabolt: An in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures |
title_full_unstemmed |
Metabolt: An in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures |
title_sort |
metabolt: an in-situ instrument to characterize the metabolic activity of microbial soil ecosystems using electrochemical and gaseous signatures |
publisher |
Molecular Diversity Preservation International |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/234090 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://dx.doi.org/10.3390/s20164479 Sí issn: 1424-8220 Sensors 20: 1- 18 (2020) http://hdl.handle.net/10261/234090 |
op_rights |
open |
op_doi |
https://doi.org/10.3390/s20164479 |
container_title |
Sensors |
container_volume |
20 |
container_issue |
16 |
container_start_page |
4479 |
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1790597540707565568 |