Changes in Activity and Community Composition Shape Bacterial Responses to Size-Fractionated Marine DOM
14 pages, 4 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) To study the response of bacteria to different size-fractions of naturally occurring dissolved organic matter (DOM), a natural prokaryotic community from Nor...
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Online Access: | http://hdl.handle.net/10261/233596 https://doi.org/10.3389/fmicb.2020.586148 |
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ftcsic:oai:digital.csic.es:10261/233596 2024-02-11T10:06:41+01:00 Changes in Activity and Community Composition Shape Bacterial Responses to Size-Fractionated Marine DOM Varela, Marta M. Rodríguez-Ramos, Tamara Guerrero-Feijóo, E. Nieto-Cid, Mar 2020 http://hdl.handle.net/10261/233596 https://doi.org/10.3389/fmicb.2020.586148 en eng Frontiers Media Publisher's version https://doi.org/10.3389/fmicb.2020.586148 Sí Frontiers in Microbiology 11: 586148 (2020) http://hdl.handle.net/10261/233596 doi:10.3389/fmicb.2020.586148 1664-302X 33329457 open Bacterial diversity Amplicon sequencing variants Flow cytometry Dissolved organic matter DOM optical properties Tangential ultrafiltration Size-fractionated DOM Remineralization artículo http://purl.org/coar/resource_type/c_6501 2020 ftcsic https://doi.org/10.3389/fmicb.2020.586148 2024-01-16T11:04:25Z 14 pages, 4 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) To study the response of bacteria to different size-fractions of naturally occurring dissolved organic matter (DOM), a natural prokaryotic community from North Atlantic mesopelagic waters (1000 m depth) was isolated and grown in (i) 0.1-μm filtered seawater (CONTROL), (ii) the low-molecular-weight (<1 kDa) DOM fraction (L-DOM), and (iii) the recombination of high- (>1 kDa) and low-molecular-weight DOM fractions (H + L-DOM), to test the potential effect of ultrafiltration on breaking the DOM size continuum. Prokaryotic abundance and leucine incorporation were consistently higher in the H + L-DOM niche than in the L-DOM and CONTROL treatments, suggesting a different interaction with each DOM fraction and the disruption of the structural DOM continuum by ultrafiltration, respectively. Rhodobacterales (Alphaproteobacteria) and Flavobacteriales (Bacteroidetes) were particularly enriched in L-DOM and closely related to the colored DOM (CDOM) fraction, indicating the tight link between these groups and changes in DOM aromaticity. Conversely, some other taxa that were rare or undetectable in the original bacterial community were enriched in the H + L-DOM treatment (e.g., Alteromonadales belonging to Gammaproteobacteria), highlighting the role of the rare biosphere as a seed bank of diversity against ecosystem disturbance. The relationship between the fluorescence of protein-like CDOM and community composition of populations in the H + L-DOM treatment suggested their preference for labile DOM. Conversely, the communities growing on the L-DOM niche were coupled to humic-like CDOM, which may indicate their ability to degrade more reworked DOM and/or the generation of refractory substrates (as by-products of the respiration processes). Most importantly, L- and/or H + L-DOM treatments stimulated the growth of unique bacterial amplicon sequence variants (ASVs), suggesting the ... Article in Journal/Newspaper North Atlantic Digital.CSIC (Spanish National Research Council) Frontiers in Microbiology 11 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Bacterial diversity Amplicon sequencing variants Flow cytometry Dissolved organic matter DOM optical properties Tangential ultrafiltration Size-fractionated DOM Remineralization |
spellingShingle |
Bacterial diversity Amplicon sequencing variants Flow cytometry Dissolved organic matter DOM optical properties Tangential ultrafiltration Size-fractionated DOM Remineralization Varela, Marta M. Rodríguez-Ramos, Tamara Guerrero-Feijóo, E. Nieto-Cid, Mar Changes in Activity and Community Composition Shape Bacterial Responses to Size-Fractionated Marine DOM |
topic_facet |
Bacterial diversity Amplicon sequencing variants Flow cytometry Dissolved organic matter DOM optical properties Tangential ultrafiltration Size-fractionated DOM Remineralization |
description |
14 pages, 4 figures, 1 table.-- This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) To study the response of bacteria to different size-fractions of naturally occurring dissolved organic matter (DOM), a natural prokaryotic community from North Atlantic mesopelagic waters (1000 m depth) was isolated and grown in (i) 0.1-μm filtered seawater (CONTROL), (ii) the low-molecular-weight (<1 kDa) DOM fraction (L-DOM), and (iii) the recombination of high- (>1 kDa) and low-molecular-weight DOM fractions (H + L-DOM), to test the potential effect of ultrafiltration on breaking the DOM size continuum. Prokaryotic abundance and leucine incorporation were consistently higher in the H + L-DOM niche than in the L-DOM and CONTROL treatments, suggesting a different interaction with each DOM fraction and the disruption of the structural DOM continuum by ultrafiltration, respectively. Rhodobacterales (Alphaproteobacteria) and Flavobacteriales (Bacteroidetes) were particularly enriched in L-DOM and closely related to the colored DOM (CDOM) fraction, indicating the tight link between these groups and changes in DOM aromaticity. Conversely, some other taxa that were rare or undetectable in the original bacterial community were enriched in the H + L-DOM treatment (e.g., Alteromonadales belonging to Gammaproteobacteria), highlighting the role of the rare biosphere as a seed bank of diversity against ecosystem disturbance. The relationship between the fluorescence of protein-like CDOM and community composition of populations in the H + L-DOM treatment suggested their preference for labile DOM. Conversely, the communities growing on the L-DOM niche were coupled to humic-like CDOM, which may indicate their ability to degrade more reworked DOM and/or the generation of refractory substrates (as by-products of the respiration processes). Most importantly, L- and/or H + L-DOM treatments stimulated the growth of unique bacterial amplicon sequence variants (ASVs), suggesting the ... |
format |
Article in Journal/Newspaper |
author |
Varela, Marta M. Rodríguez-Ramos, Tamara Guerrero-Feijóo, E. Nieto-Cid, Mar |
author_facet |
Varela, Marta M. Rodríguez-Ramos, Tamara Guerrero-Feijóo, E. Nieto-Cid, Mar |
author_sort |
Varela, Marta M. |
title |
Changes in Activity and Community Composition Shape Bacterial Responses to Size-Fractionated Marine DOM |
title_short |
Changes in Activity and Community Composition Shape Bacterial Responses to Size-Fractionated Marine DOM |
title_full |
Changes in Activity and Community Composition Shape Bacterial Responses to Size-Fractionated Marine DOM |
title_fullStr |
Changes in Activity and Community Composition Shape Bacterial Responses to Size-Fractionated Marine DOM |
title_full_unstemmed |
Changes in Activity and Community Composition Shape Bacterial Responses to Size-Fractionated Marine DOM |
title_sort |
changes in activity and community composition shape bacterial responses to size-fractionated marine dom |
publisher |
Frontiers Media |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/233596 https://doi.org/10.3389/fmicb.2020.586148 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
Publisher's version https://doi.org/10.3389/fmicb.2020.586148 Sí Frontiers in Microbiology 11: 586148 (2020) http://hdl.handle.net/10261/233596 doi:10.3389/fmicb.2020.586148 1664-302X 33329457 |
op_rights |
open |
op_doi |
https://doi.org/10.3389/fmicb.2020.586148 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
_version_ |
1790604561350656000 |