Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands
The strain KG01T was isolated from a soil sample from King George Island, Antarctica. Cells of KG01T are rod-shaped and motile by means of multiple polar flagella. The absence of arginine dihydrolase activity could be a key feature to readily distinguish KG01T from its closest phylogenetic relative...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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Microbiology Society
2020
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Online Access: | http://hdl.handle.net/10261/229684 https://doi.org/10.1099/ijsem.0.004165 https://doi.org/10.13039/501100002848 |
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ftcsic:oai:digital.csic.es:10261/229684 2024-02-11T09:55:42+01:00 Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands Pavlov, Maria S. Lira, Felipe Martínez, José Luis Olivares-Pacheco, Jorge Marshall, Sergio H. Comisión Nacional de Investigación Científica y Tecnológica (Chile) Instituto Antártico Chileno 2020 http://hdl.handle.net/10261/229684 https://doi.org/10.1099/ijsem.0.004165 https://doi.org/10.13039/501100002848 unknown Microbiology Society http://dx.doi.org/10.1099/ijsem.0.004165 Sí International Journal of Systematic and Evolutionary Microbiology 70(5): 3255-3263 (2020) 1466-5026 http://hdl.handle.net/10261/229684 doi:10.1099/ijsem.0.004165 1466-5034 http://dx.doi.org/10.13039/501100002848 none Antarctic bacteria King George Island Pseudomonas Pseudomonas fildesensis Psychrotolerant bacterium artículo http://purl.org/coar/resource_type/c_6501 2020 ftcsic https://doi.org/10.1099/ijsem.0.00416510.13039/501100002848 2024-01-16T11:02:52Z The strain KG01T was isolated from a soil sample from King George Island, Antarctica. Cells of KG01T are rod-shaped and motile by means of multiple polar flagella. The absence of arginine dihydrolase activity could be a key feature to readily distinguish KG01T from its closest phylogenetic relative species. The main fatty acids of the strain include summed feature 3 (C16 : 1 ω7c and/or C15 : 0 iso 2-OH), C16 : 0 and C18 : 1 ω7c. Phylogenetic analysis based on the 16S rRNA gene sequence and on a multilocus sequence analysis (MLSA) using housekeeping genes (16S rRNA, rpoB, rpoD, gyrB) were carried out. These analyses allowed us to include the strain within the Pseudomonas fluorescens group, presenting the highest similarity of multilocus sequence with Pseudomonas veronii LMG 17761T (96.67 %). The genome of KG01T was sequenced and in silico compared with genomes of the most closely related species of the P. fluore scens group. The average nucleotide identity (ANIb) and average amino acid identity (AAI) values of the species phylogenetically closest to KG01T were less than 95–96 %, threshold currently accepted to define strain as belonging to a bacterial species, the highest scores being those to Pseudomonas veronii LMG 17761T (87.98 %) and Pseudomonas marginalis ICMP 3553T (91.90 %). Therefore, the phenotypic and genotypic analyses results, allow us to propose that KG01T represents a member of a novel species of the genus Pseudomonas , for which the name Pseudomonas fildesensis is proposed, and KG01T (=CECT 9084T;=DSM 102036T) is established as the type strain . This study was supported by the: Soledad S Pavlov , Comisión Nacional de Investigación Científica y Tecnológica , (Award PhD scolarship) Soledad S Pavlov , INACH , (Award DT_02_13) Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island South Shetland Islands Digital.CSIC (Spanish National Research Council) Antarctic Inach ENVELOPE(-60.783,-60.783,-62.467,-62.467) King George Island South Shetland Islands International Journal of Systematic and Evolutionary Microbiology 70 5 3255 3263 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Antarctic bacteria King George Island Pseudomonas Pseudomonas fildesensis Psychrotolerant bacterium |
spellingShingle |
Antarctic bacteria King George Island Pseudomonas Pseudomonas fildesensis Psychrotolerant bacterium Pavlov, Maria S. Lira, Felipe Martínez, José Luis Olivares-Pacheco, Jorge Marshall, Sergio H. Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands |
topic_facet |
Antarctic bacteria King George Island Pseudomonas Pseudomonas fildesensis Psychrotolerant bacterium |
description |
The strain KG01T was isolated from a soil sample from King George Island, Antarctica. Cells of KG01T are rod-shaped and motile by means of multiple polar flagella. The absence of arginine dihydrolase activity could be a key feature to readily distinguish KG01T from its closest phylogenetic relative species. The main fatty acids of the strain include summed feature 3 (C16 : 1 ω7c and/or C15 : 0 iso 2-OH), C16 : 0 and C18 : 1 ω7c. Phylogenetic analysis based on the 16S rRNA gene sequence and on a multilocus sequence analysis (MLSA) using housekeeping genes (16S rRNA, rpoB, rpoD, gyrB) were carried out. These analyses allowed us to include the strain within the Pseudomonas fluorescens group, presenting the highest similarity of multilocus sequence with Pseudomonas veronii LMG 17761T (96.67 %). The genome of KG01T was sequenced and in silico compared with genomes of the most closely related species of the P. fluore scens group. The average nucleotide identity (ANIb) and average amino acid identity (AAI) values of the species phylogenetically closest to KG01T were less than 95–96 %, threshold currently accepted to define strain as belonging to a bacterial species, the highest scores being those to Pseudomonas veronii LMG 17761T (87.98 %) and Pseudomonas marginalis ICMP 3553T (91.90 %). Therefore, the phenotypic and genotypic analyses results, allow us to propose that KG01T represents a member of a novel species of the genus Pseudomonas , for which the name Pseudomonas fildesensis is proposed, and KG01T (=CECT 9084T;=DSM 102036T) is established as the type strain . This study was supported by the: Soledad S Pavlov , Comisión Nacional de Investigación Científica y Tecnológica , (Award PhD scolarship) Soledad S Pavlov , INACH , (Award DT_02_13) |
author2 |
Comisión Nacional de Investigación Científica y Tecnológica (Chile) Instituto Antártico Chileno |
format |
Article in Journal/Newspaper |
author |
Pavlov, Maria S. Lira, Felipe Martínez, José Luis Olivares-Pacheco, Jorge Marshall, Sergio H. |
author_facet |
Pavlov, Maria S. Lira, Felipe Martínez, José Luis Olivares-Pacheco, Jorge Marshall, Sergio H. |
author_sort |
Pavlov, Maria S. |
title |
Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands |
title_short |
Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands |
title_full |
Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands |
title_fullStr |
Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands |
title_full_unstemmed |
Pseudomonas fildesensis sp. Nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, South Shetland Islands |
title_sort |
pseudomonas fildesensis sp. nov., a psychrotolerant bacterium isolated from antarctic soil of king george island, south shetland islands |
publisher |
Microbiology Society |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/229684 https://doi.org/10.1099/ijsem.0.004165 https://doi.org/10.13039/501100002848 |
long_lat |
ENVELOPE(-60.783,-60.783,-62.467,-62.467) |
geographic |
Antarctic Inach King George Island South Shetland Islands |
geographic_facet |
Antarctic Inach King George Island South Shetland Islands |
genre |
Antarc* Antarctic Antarctica King George Island South Shetland Islands |
genre_facet |
Antarc* Antarctic Antarctica King George Island South Shetland Islands |
op_relation |
http://dx.doi.org/10.1099/ijsem.0.004165 Sí International Journal of Systematic and Evolutionary Microbiology 70(5): 3255-3263 (2020) 1466-5026 http://hdl.handle.net/10261/229684 doi:10.1099/ijsem.0.004165 1466-5034 http://dx.doi.org/10.13039/501100002848 |
op_rights |
none |
op_doi |
https://doi.org/10.1099/ijsem.0.00416510.13039/501100002848 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
70 |
container_issue |
5 |
container_start_page |
3255 |
op_container_end_page |
3263 |
_version_ |
1790598361654493184 |