Role of the spleen in the immune response of turbot (Scophthalmus maximus) to vaccination with the ciliate parasite Philasterides dicentrarchi
Trabajo presentado en la 3rd Conference of the International Society of Fish & Shellfish Immunology (ISFSI), celebrada en Las Palmas de Gran Canaria (España) del 16 al 20 de junio de 2019. Fish spleen is rich in lymphocytes, particularly in B lymphocytes, and plays an important role in the adapt...
Published in: | Fish & Shellfish Immunology |
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Elsevier
2019
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Online Access: | http://hdl.handle.net/10261/227260 https://doi.org/10.1016/j.fsi.2019.04.111 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003359 https://doi.org/10.13039/501100010801 |
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ftcsic:oai:digital.csic.es:10261/227260 2024-02-11T10:08:28+01:00 Role of the spleen in the immune response of turbot (Scophthalmus maximus) to vaccination with the ciliate parasite Philasterides dicentrarchi Fontenla, F. Estensoro, Itziar Valle, A. Noia, M. Tafalla, Carolina Sitjà-Bobadilla, Ariadna Leiro, José-Manuel Lamas, Jesús European Commission Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Xunta de Galicia Generalitat Valenciana 2019-06-17 http://hdl.handle.net/10261/227260 https://doi.org/10.1016/j.fsi.2019.04.111 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003359 https://doi.org/10.13039/501100010801 unknown Elsevier #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/634429 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-83577-R AGL2017-83577-R/AEI/10.13039/501100011033 Fish & Shellfish Immunology 91: 404-405 (2019) http://dx.doi.org/https://doi.org/10.1016/j.fsi.2019.04.111 Sí 3rd International Conference on Fish and Shellfish Immunology (2019) 1050-4648 http://hdl.handle.net/10261/227260 doi:10.1016/j.fsi.2019.04.111 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100010801 open Turbot Spleen Vaccination B lymphocytes Immunoglobulins póster de congreso http://purl.org/coar/resource_type/c_6670 2019 ftcsic https://doi.org/10.1016/j.fsi.2019.04.11110.13039/50110001103310.13039/50110000078010.13039/50110000335910.13039/501100010801 2024-01-16T11:01:37Z Trabajo presentado en la 3rd Conference of the International Society of Fish & Shellfish Immunology (ISFSI), celebrada en Las Palmas de Gran Canaria (España) del 16 al 20 de junio de 2019. Fish spleen is rich in lymphocytes, particularly in B lymphocytes, and plays an important role in the adaptive immune response after vaccination. After vaccination of fish by intraperitoneal injection, there is a strong migration of antigen-containing cells to the spleen, where antigen presentation occurs. In the present study, we analysed the B cell populations (IgM, IgT or IgD positive cells) and gene expression (IgM, IgT, IgD, MHCII, and several immune-related genes) in the spleen of turbot immunized with a vaccine containing an oleous adjuvant and a particulate Philasterides dicentrarchi antigen. Fish were immunized on days 0 and 30, and samples were obtained on days 3, 7, 33, 37 and 60. The vaccine provoked a significant increase in specific and total serum IgM at 37 dpi and at 60 dpi, but the specific IgT levels did not vary significantly in vaccinated fish. No significant regulation of sIgT, mIgT, sIgM, mIgM and IgD occurred before 37 dpi, whereas at 37 and 60 dpi, overexpression of sIgT and mIgT was detected in fish injected with adjuvant alone or with the vaccine. Immunofluorescence analysis enabled classification of the B lymphocytes into four groups: IgM+IgD-IgT- (the majority of IgM+ cells), IgM+IgD+IgT-, IgM-IgD+IgT- (very few cells, probably corresponding to lymphocytes with low levels of IgM) and IgM-IgD-IgT+ cells. The IgM+ and IgT+ cells were scattered throughout the parenchyma, and grouped around large vessels and surrounding melanomacrophage centres (MMC). Cell proliferation was estimated using a combination of anti-IgT, anti-IgM and anti-PCNA antibodies. Proliferation of both IgM+ and IgT+ B cells was observed in several areas of the spleen. In addition, vaccinated fish showed a mass of vaccine and cells (CVM) associated to the spleen. The CVM contained scattered PCNA+/IgM + and PCNA+/ IgT+ B cells, ... Still Image Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Las Palmas ENVELOPE(-60.674,-60.674,-62.971,-62.971) Fish & Shellfish Immunology 91 404 405 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
unknown |
topic |
Turbot Spleen Vaccination B lymphocytes Immunoglobulins |
spellingShingle |
Turbot Spleen Vaccination B lymphocytes Immunoglobulins Fontenla, F. Estensoro, Itziar Valle, A. Noia, M. Tafalla, Carolina Sitjà-Bobadilla, Ariadna Leiro, José-Manuel Lamas, Jesús Role of the spleen in the immune response of turbot (Scophthalmus maximus) to vaccination with the ciliate parasite Philasterides dicentrarchi |
topic_facet |
Turbot Spleen Vaccination B lymphocytes Immunoglobulins |
description |
Trabajo presentado en la 3rd Conference of the International Society of Fish & Shellfish Immunology (ISFSI), celebrada en Las Palmas de Gran Canaria (España) del 16 al 20 de junio de 2019. Fish spleen is rich in lymphocytes, particularly in B lymphocytes, and plays an important role in the adaptive immune response after vaccination. After vaccination of fish by intraperitoneal injection, there is a strong migration of antigen-containing cells to the spleen, where antigen presentation occurs. In the present study, we analysed the B cell populations (IgM, IgT or IgD positive cells) and gene expression (IgM, IgT, IgD, MHCII, and several immune-related genes) in the spleen of turbot immunized with a vaccine containing an oleous adjuvant and a particulate Philasterides dicentrarchi antigen. Fish were immunized on days 0 and 30, and samples were obtained on days 3, 7, 33, 37 and 60. The vaccine provoked a significant increase in specific and total serum IgM at 37 dpi and at 60 dpi, but the specific IgT levels did not vary significantly in vaccinated fish. No significant regulation of sIgT, mIgT, sIgM, mIgM and IgD occurred before 37 dpi, whereas at 37 and 60 dpi, overexpression of sIgT and mIgT was detected in fish injected with adjuvant alone or with the vaccine. Immunofluorescence analysis enabled classification of the B lymphocytes into four groups: IgM+IgD-IgT- (the majority of IgM+ cells), IgM+IgD+IgT-, IgM-IgD+IgT- (very few cells, probably corresponding to lymphocytes with low levels of IgM) and IgM-IgD-IgT+ cells. The IgM+ and IgT+ cells were scattered throughout the parenchyma, and grouped around large vessels and surrounding melanomacrophage centres (MMC). Cell proliferation was estimated using a combination of anti-IgT, anti-IgM and anti-PCNA antibodies. Proliferation of both IgM+ and IgT+ B cells was observed in several areas of the spleen. In addition, vaccinated fish showed a mass of vaccine and cells (CVM) associated to the spleen. The CVM contained scattered PCNA+/IgM + and PCNA+/ IgT+ B cells, ... |
author2 |
European Commission Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Xunta de Galicia Generalitat Valenciana |
format |
Still Image |
author |
Fontenla, F. Estensoro, Itziar Valle, A. Noia, M. Tafalla, Carolina Sitjà-Bobadilla, Ariadna Leiro, José-Manuel Lamas, Jesús |
author_facet |
Fontenla, F. Estensoro, Itziar Valle, A. Noia, M. Tafalla, Carolina Sitjà-Bobadilla, Ariadna Leiro, José-Manuel Lamas, Jesús |
author_sort |
Fontenla, F. |
title |
Role of the spleen in the immune response of turbot (Scophthalmus maximus) to vaccination with the ciliate parasite Philasterides dicentrarchi |
title_short |
Role of the spleen in the immune response of turbot (Scophthalmus maximus) to vaccination with the ciliate parasite Philasterides dicentrarchi |
title_full |
Role of the spleen in the immune response of turbot (Scophthalmus maximus) to vaccination with the ciliate parasite Philasterides dicentrarchi |
title_fullStr |
Role of the spleen in the immune response of turbot (Scophthalmus maximus) to vaccination with the ciliate parasite Philasterides dicentrarchi |
title_full_unstemmed |
Role of the spleen in the immune response of turbot (Scophthalmus maximus) to vaccination with the ciliate parasite Philasterides dicentrarchi |
title_sort |
role of the spleen in the immune response of turbot (scophthalmus maximus) to vaccination with the ciliate parasite philasterides dicentrarchi |
publisher |
Elsevier |
publishDate |
2019 |
url |
http://hdl.handle.net/10261/227260 https://doi.org/10.1016/j.fsi.2019.04.111 https://doi.org/10.13039/501100011033 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003359 https://doi.org/10.13039/501100010801 |
long_lat |
ENVELOPE(-60.674,-60.674,-62.971,-62.971) |
geographic |
Las Palmas |
geographic_facet |
Las Palmas |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/634429 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/AGL2017-83577-R AGL2017-83577-R/AEI/10.13039/501100011033 Fish & Shellfish Immunology 91: 404-405 (2019) http://dx.doi.org/https://doi.org/10.1016/j.fsi.2019.04.111 Sí 3rd International Conference on Fish and Shellfish Immunology (2019) 1050-4648 http://hdl.handle.net/10261/227260 doi:10.1016/j.fsi.2019.04.111 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100010801 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.fsi.2019.04.11110.13039/50110001103310.13039/50110000078010.13039/50110000335910.13039/501100010801 |
container_title |
Fish & Shellfish Immunology |
container_volume |
91 |
container_start_page |
404 |
op_container_end_page |
405 |
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1790607810087616512 |