Optimization of sperm cryopreservation protocol for peregrine falcon (Falco peregrinus)
This article belongs to the Special Issue Reproductive Biotechnology in Wildlife. Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for st...
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Multidisciplinary Digital Publishing Institute
2020
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Online Access: | http://hdl.handle.net/10261/221569 https://doi.org/10.3390/ani10040691 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003329 |
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ftcsic:oai:digital.csic.es:10261/221569 2024-02-11T10:03:40+01:00 Optimization of sperm cryopreservation protocol for peregrine falcon (Falco peregrinus) Cardoso, Beatriz Sánchez-Ajofrín, Irene Castaño, Cristina García-Álvarez, Olga Esteso, Milagros C. Maroto-Morales, Alejandro Iniesta-Cuerda, María Garde, José Julián Santiago-Moreno, Julián Soler, Ana J. Ministerio de Economía y Competitividad (España) European Commission 2020 http://hdl.handle.net/10261/221569 https://doi.org/10.3390/ani10040691 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003329 en eng Multidisciplinary Digital Publishing Institute Publisher's version https://doi.org/10.3390/ani10040691 Sí Animals 10(4): 691 (2020) http://hdl.handle.net/10261/221569 doi:10.3390/ani10040691 2076-2615 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 32316152 open Cryopreservation Cryoprotectant Peregrine falcon Spermatozoa artículo http://purl.org/coar/resource_type/c_6501 2020 ftcsic https://doi.org/10.3390/ani1004069110.13039/50110000078010.13039/501100003329 2024-01-16T10:58:46Z This article belongs to the Special Issue Reproductive Biotechnology in Wildlife. Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 °C for 30 s vs. 5 °C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. This study was funded by limited company Sevilla Falcons S.L. (UCTR190025). Beatriz Cardoso was supported by the European Union Erasmus + program. María Iniesta-Cuerda was supported by a Ministry of Economy and Competitiveness scholarship. Peer reviewed Article in Journal/Newspaper Falco peregrinus peregrine falcon Digital.CSIC (Spanish National Research Council) Animals 10 4 691 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Cryopreservation Cryoprotectant Peregrine falcon Spermatozoa |
spellingShingle |
Cryopreservation Cryoprotectant Peregrine falcon Spermatozoa Cardoso, Beatriz Sánchez-Ajofrín, Irene Castaño, Cristina García-Álvarez, Olga Esteso, Milagros C. Maroto-Morales, Alejandro Iniesta-Cuerda, María Garde, José Julián Santiago-Moreno, Julián Soler, Ana J. Optimization of sperm cryopreservation protocol for peregrine falcon (Falco peregrinus) |
topic_facet |
Cryopreservation Cryoprotectant Peregrine falcon Spermatozoa |
description |
This article belongs to the Special Issue Reproductive Biotechnology in Wildlife. Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 °C for 30 s vs. 5 °C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. This study was funded by limited company Sevilla Falcons S.L. (UCTR190025). Beatriz Cardoso was supported by the European Union Erasmus + program. María Iniesta-Cuerda was supported by a Ministry of Economy and Competitiveness scholarship. Peer reviewed |
author2 |
Ministerio de Economía y Competitividad (España) European Commission |
format |
Article in Journal/Newspaper |
author |
Cardoso, Beatriz Sánchez-Ajofrín, Irene Castaño, Cristina García-Álvarez, Olga Esteso, Milagros C. Maroto-Morales, Alejandro Iniesta-Cuerda, María Garde, José Julián Santiago-Moreno, Julián Soler, Ana J. |
author_facet |
Cardoso, Beatriz Sánchez-Ajofrín, Irene Castaño, Cristina García-Álvarez, Olga Esteso, Milagros C. Maroto-Morales, Alejandro Iniesta-Cuerda, María Garde, José Julián Santiago-Moreno, Julián Soler, Ana J. |
author_sort |
Cardoso, Beatriz |
title |
Optimization of sperm cryopreservation protocol for peregrine falcon (Falco peregrinus) |
title_short |
Optimization of sperm cryopreservation protocol for peregrine falcon (Falco peregrinus) |
title_full |
Optimization of sperm cryopreservation protocol for peregrine falcon (Falco peregrinus) |
title_fullStr |
Optimization of sperm cryopreservation protocol for peregrine falcon (Falco peregrinus) |
title_full_unstemmed |
Optimization of sperm cryopreservation protocol for peregrine falcon (Falco peregrinus) |
title_sort |
optimization of sperm cryopreservation protocol for peregrine falcon (falco peregrinus) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/221569 https://doi.org/10.3390/ani10040691 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100003329 |
genre |
Falco peregrinus peregrine falcon |
genre_facet |
Falco peregrinus peregrine falcon |
op_relation |
Publisher's version https://doi.org/10.3390/ani10040691 Sí Animals 10(4): 691 (2020) http://hdl.handle.net/10261/221569 doi:10.3390/ani10040691 2076-2615 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 32316152 |
op_rights |
open |
op_doi |
https://doi.org/10.3390/ani1004069110.13039/50110000078010.13039/501100003329 |
container_title |
Animals |
container_volume |
10 |
container_issue |
4 |
container_start_page |
691 |
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1790599985930174464 |