Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
Omega-3 (ω3 or n-3) long-chain polyunsaturated fatty acids (PUFA) including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to be originated almost exclusively from aquatic (mostly marine) single-cell...
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Royal Society (Great Britain)
2020
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Online Access: | http://hdl.handle.net/10261/218918 https://doi.org/10.1098/rstb.2019.0654 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100001691 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100000867 https://doi.org/10.13039/501100005416 https://doi.org/10.13039/501100011033 |
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ftcsic:oai:digital.csic.es:10261/218918 2024-02-11T10:06:41+01:00 Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading Kabeya, Naoki Gür, İbrahim Oboh, Angela Ove Evjemo, Jan Malzahn, Arne M. Hontoria, Francisco Navarro, Juan Carlos Monroig, Óscar Ministerio de Ciencia e Innovación (España) European Commission Japan Society for the Promotion of Science Commonwealth Scholarship Commission Norwegian Research Council Agencia Estatal de Investigación (España) Hontoria, Francisco Navarro, Juan Carlos Monroig, Óscar 2020-08-03 http://hdl.handle.net/10261/218918 https://doi.org/10.1098/rstb.2019.0654 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100001691 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100000867 https://doi.org/10.13039/501100005416 https://doi.org/10.13039/501100011033 es spa Royal Society (Great Britain) #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095119-B-I00 RTI2018-095119-B-I00/AEI/10.13039/501100011033 Postprint http://dx.doi.org/10.1098/rstb.2019.0654 Sí Philosophical Transactions - B - Biological Sciences 375:1804 (2020) 0962-8436 http://hdl.handle.net/10261/218918 doi:10.1098/rstb.2019.0654 1471-2970 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100001691 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100000867 http://dx.doi.org/10.13039/501100005416 http://dx.doi.org/10.13039/501100011033 32536307 open Biosynthesis Methyl-end desaturase N-3 long-chain PUFA Polychaetes artículo http://purl.org/coar/resource_type/c_6501 2020 ftcsic https://doi.org/10.1098/rstb.2019.065410.13039/50110000483710.13039/50110000169110.13039/50110000078010.13039/50110000086710.13039/50110000541610.13039/501100011033 2024-01-16T10:57:22Z Omega-3 (ω3 or n-3) long-chain polyunsaturated fatty acids (PUFA) including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to be originated almost exclusively from aquatic (mostly marine) single-cell organisms. Yet, a recent study has discovered that many invertebrates possess a type of enzymes called methyl-end desaturases (ωx) that enables them to endogenously produce n-3 long-chain PUFA and could make a significant contribution to production of these compounds in the marine environment. Polychaetes are major components of benthic fauna and thus important to maintain a robust food web as a recycler of organic matter and a prey item for higher trophic level species like fish. In the present study, we investigated the ωx enzymes from the common ragworm Hediste diversicolor, a common inhabitant in sedimentary littoral ecosystems of the North Atlantic. Functional assays of the H. diversicolor ωx demonstrated unique desaturation capacities. An ω3 desaturase mediated the conversion of n-6 fatty acid substrates into their corresponding n-3 products including DHA. A further enzyme possessed unique regioselectivities combining both ω6 and ω3 desaturase activities. These results illustrate that the long-chain PUFA biosynthetic enzymatic machinery of aquatic invertebrates such as polychaetes is highly diverse and clarify that invertebrates can be major contributors to fatty acid trophic upgrading in aquatic food webs. This study was funded through the project IMPROMEGA of the Ministry of Science, Innovation and Universities, Spanish Government (grant no. RTI2018-095119-B-I00, MCIU/AEI/FEDER, UE). N.K. was funded by the Japan Society for the Promotion of Science through Grant-in-Aid for JSPS Fellows (grant no. 16J06812). İ.G. received funding from the Ministry of National Education, Republic of Turkey. A.O. was funded by the Commonwealth Scholarship Commission (grant no. NGCS-2014-438) in the UK. A.M.M. acknowledges the ... Article in Journal/Newspaper North Atlantic Digital.CSIC (Spanish National Research Council) |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
Spanish |
topic |
Biosynthesis Methyl-end desaturase N-3 long-chain PUFA Polychaetes |
spellingShingle |
Biosynthesis Methyl-end desaturase N-3 long-chain PUFA Polychaetes Kabeya, Naoki Gür, İbrahim Oboh, Angela Ove Evjemo, Jan Malzahn, Arne M. Hontoria, Francisco Navarro, Juan Carlos Monroig, Óscar Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
topic_facet |
Biosynthesis Methyl-end desaturase N-3 long-chain PUFA Polychaetes |
description |
Omega-3 (ω3 or n-3) long-chain polyunsaturated fatty acids (PUFA) including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to be originated almost exclusively from aquatic (mostly marine) single-cell organisms. Yet, a recent study has discovered that many invertebrates possess a type of enzymes called methyl-end desaturases (ωx) that enables them to endogenously produce n-3 long-chain PUFA and could make a significant contribution to production of these compounds in the marine environment. Polychaetes are major components of benthic fauna and thus important to maintain a robust food web as a recycler of organic matter and a prey item for higher trophic level species like fish. In the present study, we investigated the ωx enzymes from the common ragworm Hediste diversicolor, a common inhabitant in sedimentary littoral ecosystems of the North Atlantic. Functional assays of the H. diversicolor ωx demonstrated unique desaturation capacities. An ω3 desaturase mediated the conversion of n-6 fatty acid substrates into their corresponding n-3 products including DHA. A further enzyme possessed unique regioselectivities combining both ω6 and ω3 desaturase activities. These results illustrate that the long-chain PUFA biosynthetic enzymatic machinery of aquatic invertebrates such as polychaetes is highly diverse and clarify that invertebrates can be major contributors to fatty acid trophic upgrading in aquatic food webs. This study was funded through the project IMPROMEGA of the Ministry of Science, Innovation and Universities, Spanish Government (grant no. RTI2018-095119-B-I00, MCIU/AEI/FEDER, UE). N.K. was funded by the Japan Society for the Promotion of Science through Grant-in-Aid for JSPS Fellows (grant no. 16J06812). İ.G. received funding from the Ministry of National Education, Republic of Turkey. A.O. was funded by the Commonwealth Scholarship Commission (grant no. NGCS-2014-438) in the UK. A.M.M. acknowledges the ... |
author2 |
Ministerio de Ciencia e Innovación (España) European Commission Japan Society for the Promotion of Science Commonwealth Scholarship Commission Norwegian Research Council Agencia Estatal de Investigación (España) Hontoria, Francisco Navarro, Juan Carlos Monroig, Óscar |
format |
Article in Journal/Newspaper |
author |
Kabeya, Naoki Gür, İbrahim Oboh, Angela Ove Evjemo, Jan Malzahn, Arne M. Hontoria, Francisco Navarro, Juan Carlos Monroig, Óscar |
author_facet |
Kabeya, Naoki Gür, İbrahim Oboh, Angela Ove Evjemo, Jan Malzahn, Arne M. Hontoria, Francisco Navarro, Juan Carlos Monroig, Óscar |
author_sort |
Kabeya, Naoki |
title |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_short |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_full |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_fullStr |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_full_unstemmed |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_sort |
unique fatty acid desaturase capacities uncovered in hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
publisher |
Royal Society (Great Britain) |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/218918 https://doi.org/10.1098/rstb.2019.0654 https://doi.org/10.13039/501100004837 https://doi.org/10.13039/501100001691 https://doi.org/10.13039/501100000780 https://doi.org/10.13039/501100000867 https://doi.org/10.13039/501100005416 https://doi.org/10.13039/501100011033 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
#PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095119-B-I00 RTI2018-095119-B-I00/AEI/10.13039/501100011033 Postprint http://dx.doi.org/10.1098/rstb.2019.0654 Sí Philosophical Transactions - B - Biological Sciences 375:1804 (2020) 0962-8436 http://hdl.handle.net/10261/218918 doi:10.1098/rstb.2019.0654 1471-2970 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100001691 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100000867 http://dx.doi.org/10.13039/501100005416 http://dx.doi.org/10.13039/501100011033 32536307 |
op_rights |
open |
op_doi |
https://doi.org/10.1098/rstb.2019.065410.13039/50110000483710.13039/50110000169110.13039/50110000078010.13039/50110000086710.13039/50110000541610.13039/501100011033 |
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