Biotechnological production of zeaxanthin by an Antarctic Flavobacterium: Evaluation of culture conditions
24 Páginas.-- 1 Figura.-- 7 Tablas Statistical experimental designs were used to formulate a culture medium for zeaxanthin production by an Antarctic Flavobacterium sp. P8 strain. Eleven nutritional factors were assayed in shaken flasks. The effect of temperature on zeaxanthin and carotenoid product...
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ftcsic:oai:digital.csic.es:10261/215553 2024-02-11T09:57:19+01:00 Biotechnological production of zeaxanthin by an Antarctic Flavobacterium: Evaluation of culture conditions Vila, Eugenia Hornero-Méndez, Dámaso Lareo, Claudia Saravia, Verónica Comisión Interministerial de Ciencia y Tecnología, CICYT (España) 2020-05-22 http://hdl.handle.net/10261/215553 https://doi.org/10.13039/501100007273 en eng Elsevier Postprint http://dx.doi.org/10.1016/j.jbiotec.2020.05.014 Sí Journal of Biotechnology 319: 54 (2020) 0168-165 http://hdl.handle.net/10261/215553 1873-4863 http://dx.doi.org/10.13039/501100007273 open Carotenoids Zeaxanthin Flavobacteriumexperimental design Bioreactor artículo http://purl.org/coar/resource_type/c_6501 2020 ftcsic https://doi.org/10.13039/50110000727310.1016/j.jbiotec.2020.05.014 2024-01-16T10:55:52Z 24 Páginas.-- 1 Figura.-- 7 Tablas Statistical experimental designs were used to formulate a culture medium for zeaxanthin production by an Antarctic Flavobacterium sp. P8 strain. Eleven nutritional factors were assayed in shaken flasks. The effect of temperature on zeaxanthin and carotenoid production was also studied. Peptone, yeast extract, and sodium chloride were the nutrients that caused the principal impact on the biomass growth. These components were further studied to enhance zeaxanthin and total carotenoid concentrations. Although a high production rate of zeaxanthin and carotenoids was achieved, the aerobic characteristics of the bacterial strain and the oxygen requirements for zeaxanthin biosynthesis incorporate a factor that requires additional consideration. Scaling up the process to a 5 L-bioreactor that increased dissolved oxygen availability resulted in a 4.5-fold increase in the total carotenoid content and an almost 9-fold increase in zeaxanthin, which represented 98% of the total carotenoids produced. The results reveal that Flavobacterium sp. P8 is a promising strain for zeaxanthin production. The authors would like to thank Comisión Académica de Posgrado (CAP) for the postgraduate scholarship given to Eugenia Vila and the Instituto Antártico Uruguayo and Comisión Sectorial de Investigación Científica (CSIC) for their financial support (CSIC I+D 2014 219, CSIC INI 230). Peer reviewed Article in Journal/Newspaper Antarc* Antarctic Digital.CSIC (Spanish National Research Council) Antarctic |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Carotenoids Zeaxanthin Flavobacteriumexperimental design Bioreactor |
spellingShingle |
Carotenoids Zeaxanthin Flavobacteriumexperimental design Bioreactor Vila, Eugenia Hornero-Méndez, Dámaso Lareo, Claudia Saravia, Verónica Biotechnological production of zeaxanthin by an Antarctic Flavobacterium: Evaluation of culture conditions |
topic_facet |
Carotenoids Zeaxanthin Flavobacteriumexperimental design Bioreactor |
description |
24 Páginas.-- 1 Figura.-- 7 Tablas Statistical experimental designs were used to formulate a culture medium for zeaxanthin production by an Antarctic Flavobacterium sp. P8 strain. Eleven nutritional factors were assayed in shaken flasks. The effect of temperature on zeaxanthin and carotenoid production was also studied. Peptone, yeast extract, and sodium chloride were the nutrients that caused the principal impact on the biomass growth. These components were further studied to enhance zeaxanthin and total carotenoid concentrations. Although a high production rate of zeaxanthin and carotenoids was achieved, the aerobic characteristics of the bacterial strain and the oxygen requirements for zeaxanthin biosynthesis incorporate a factor that requires additional consideration. Scaling up the process to a 5 L-bioreactor that increased dissolved oxygen availability resulted in a 4.5-fold increase in the total carotenoid content and an almost 9-fold increase in zeaxanthin, which represented 98% of the total carotenoids produced. The results reveal that Flavobacterium sp. P8 is a promising strain for zeaxanthin production. The authors would like to thank Comisión Académica de Posgrado (CAP) for the postgraduate scholarship given to Eugenia Vila and the Instituto Antártico Uruguayo and Comisión Sectorial de Investigación Científica (CSIC) for their financial support (CSIC I+D 2014 219, CSIC INI 230). Peer reviewed |
author2 |
Comisión Interministerial de Ciencia y Tecnología, CICYT (España) |
format |
Article in Journal/Newspaper |
author |
Vila, Eugenia Hornero-Méndez, Dámaso Lareo, Claudia Saravia, Verónica |
author_facet |
Vila, Eugenia Hornero-Méndez, Dámaso Lareo, Claudia Saravia, Verónica |
author_sort |
Vila, Eugenia |
title |
Biotechnological production of zeaxanthin by an Antarctic Flavobacterium: Evaluation of culture conditions |
title_short |
Biotechnological production of zeaxanthin by an Antarctic Flavobacterium: Evaluation of culture conditions |
title_full |
Biotechnological production of zeaxanthin by an Antarctic Flavobacterium: Evaluation of culture conditions |
title_fullStr |
Biotechnological production of zeaxanthin by an Antarctic Flavobacterium: Evaluation of culture conditions |
title_full_unstemmed |
Biotechnological production of zeaxanthin by an Antarctic Flavobacterium: Evaluation of culture conditions |
title_sort |
biotechnological production of zeaxanthin by an antarctic flavobacterium: evaluation of culture conditions |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/215553 https://doi.org/10.13039/501100007273 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Postprint http://dx.doi.org/10.1016/j.jbiotec.2020.05.014 Sí Journal of Biotechnology 319: 54 (2020) 0168-165 http://hdl.handle.net/10261/215553 1873-4863 http://dx.doi.org/10.13039/501100007273 |
op_rights |
open |
op_doi |
https://doi.org/10.13039/50110000727310.1016/j.jbiotec.2020.05.014 |
_version_ |
1790609608245510144 |