Isolating Marine Microorganisms from the Deep Sea

• Main Author: Sanz-Sáez, Isabel
• Other Authors: Acinas, Silvia G., Sánchez, Olga
• Format: Master Thesis
• Language: unknown
• Published: Universidad Autónoma de Barcelona 2015
• Subjects: Martínez; Silvia; South Atlantic Ocean
• Online Access: http://hdl.handle.net/10261/211907

Trabajo final presentado por Isabel Sanz Saez para un Máster de la Universitat Autònoma de Barcelona (UAB), realizado bajo la dirección de la Dra. Silvia G. Acinas y la Dra. Olga Sánchez Martínez del Institut de Ciències del Mar (ICM-CSIC).-- 6 pages, 4 figures Isolation of marinemicroorganisms is fundamental to infer the physiology, ecology and genome content. Although many new bacterial isolates are identified from surface waters a minor effort has been done in marine samples from the deep ocean. In this study we have isolated more than 500 bathypelagic isolates from the Mediterranean Sea (2000 m) and two contrasted samples from the South Atlantic Ocean (4000 m depth). It has been partially sequenced the 16S rRNA gene and conducted phylogenetic analyses to infer their relationship between them and their closest relatives from public datasets. It has been uncovered many unique groups of isolates specific from the deep ocean related to different taxonomic groups, such as Alteromonas, Pseudoalteromonas, Marinobacter, Alcanivoraxbut also observed groups of isolates well adapted to live in both environments (surface and deep) and therefore adapted to different temperatures and highly versatile in their metabolic requirements. Some of these isolates show the potential for the degradation of polycyclic aromatic hydrocarbons (PAHs), an important component present in petroleum,and they would be further explored in future studies