Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption
3rd International Symposium on Fish & Shellfish Immunology, June 16th-20th 2019, Las Palmas de Gran Canaria (Spain) P. dicentrarchi is one of the most threatening pathogens for turbot aquaculture. This protozoan ciliate is a causative agent of scuticociliatosis, which is a disease with important...
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ftcsic:oai:digital.csic.es:10261/203745 2024-02-11T10:08:27+01:00 Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption Lama, R. Pereiro, Patricia Costa, M. M. Encinar, José Antonio Medina-Gali, Regla María Lamas, J. Leiro, J. Figueras Huerta, Antonio Novoa, Beatriz 2019 http://hdl.handle.net/10261/203745 en eng Sí 3rd International Symposium on Fish & Shellfish Immunology (2019) http://hdl.handle.net/10261/203745 none Philasterides dicentrarchi Turbot Nk-lysin Antimicrobial peptides Antiparasitic comunicación de congreso http://purl.org/coar/resource_type/c_5794 2019 ftcsic 2024-01-16T10:49:58Z 3rd International Symposium on Fish & Shellfish Immunology, June 16th-20th 2019, Las Palmas de Gran Canaria (Spain) P. dicentrarchi is one of the most threatening pathogens for turbot aquaculture. This protozoan ciliate is a causative agent of scuticociliatosis, which is a disease with important economic consequences for the sector. Neither vaccines nor therapeutic treatments are commercially available to combat this infection. Numerous antimicrobial peptides (AMPs) have demonstrated broad-spectrum activity against bacteria, viruses, fungi, parasites and even tumor cells; an example is Nk-lysin (Nkl), which is an AMP belonging to the saposin-like protein (SAPLIP) family with an ability to interact with biological membranes. Following the recent characterization of turbot Nkl, an expression plasmid encoding Nkl was constructed and an anti-Nkl polyclonal antibody was successfully tested. Using these tools, we demonstrated that although infection did not clearly affect nkl mRNA expression, it induced changes at the protein level. Turbot Nkl had the ability to inhibit proliferation of the P. dicentrarchi parasite both in vivo and in vitro. Moreover, a shortened peptide containing the active core of turbot Nkl (Nkl71-100) was synthesized and showed high antiparasitic activity with a direct effect on parasite viability that probably occurred via membrane disruption. Therefore, the nkl gene may be a good candidate for genetic breeding selection of fish, and either the encoded peptide or its shortened analog is a promising antiparasitic treatment in aquaculture Peer reviewed Conference Object Scophthalmus maximus Turbot Digital.CSIC (Spanish National Research Council) Las Palmas ENVELOPE(-60.674,-60.674,-62.971,-62.971) |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Philasterides dicentrarchi Turbot Nk-lysin Antimicrobial peptides Antiparasitic |
spellingShingle |
Philasterides dicentrarchi Turbot Nk-lysin Antimicrobial peptides Antiparasitic Lama, R. Pereiro, Patricia Costa, M. M. Encinar, José Antonio Medina-Gali, Regla María Lamas, J. Leiro, J. Figueras Huerta, Antonio Novoa, Beatriz Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption |
topic_facet |
Philasterides dicentrarchi Turbot Nk-lysin Antimicrobial peptides Antiparasitic |
description |
3rd International Symposium on Fish & Shellfish Immunology, June 16th-20th 2019, Las Palmas de Gran Canaria (Spain) P. dicentrarchi is one of the most threatening pathogens for turbot aquaculture. This protozoan ciliate is a causative agent of scuticociliatosis, which is a disease with important economic consequences for the sector. Neither vaccines nor therapeutic treatments are commercially available to combat this infection. Numerous antimicrobial peptides (AMPs) have demonstrated broad-spectrum activity against bacteria, viruses, fungi, parasites and even tumor cells; an example is Nk-lysin (Nkl), which is an AMP belonging to the saposin-like protein (SAPLIP) family with an ability to interact with biological membranes. Following the recent characterization of turbot Nkl, an expression plasmid encoding Nkl was constructed and an anti-Nkl polyclonal antibody was successfully tested. Using these tools, we demonstrated that although infection did not clearly affect nkl mRNA expression, it induced changes at the protein level. Turbot Nkl had the ability to inhibit proliferation of the P. dicentrarchi parasite both in vivo and in vitro. Moreover, a shortened peptide containing the active core of turbot Nkl (Nkl71-100) was synthesized and showed high antiparasitic activity with a direct effect on parasite viability that probably occurred via membrane disruption. Therefore, the nkl gene may be a good candidate for genetic breeding selection of fish, and either the encoded peptide or its shortened analog is a promising antiparasitic treatment in aquaculture Peer reviewed |
format |
Conference Object |
author |
Lama, R. Pereiro, Patricia Costa, M. M. Encinar, José Antonio Medina-Gali, Regla María Lamas, J. Leiro, J. Figueras Huerta, Antonio Novoa, Beatriz |
author_facet |
Lama, R. Pereiro, Patricia Costa, M. M. Encinar, José Antonio Medina-Gali, Regla María Lamas, J. Leiro, J. Figueras Huerta, Antonio Novoa, Beatriz |
author_sort |
Lama, R. |
title |
Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption |
title_short |
Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption |
title_full |
Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption |
title_fullStr |
Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption |
title_full_unstemmed |
Turbot (Scophthalmus maximus) Nk-lysin induces protection against the pathogenic parasite Philasterides dicentrarchi via membrane disruption |
title_sort |
turbot (scophthalmus maximus) nk-lysin induces protection against the pathogenic parasite philasterides dicentrarchi via membrane disruption |
publishDate |
2019 |
url |
http://hdl.handle.net/10261/203745 |
long_lat |
ENVELOPE(-60.674,-60.674,-62.971,-62.971) |
geographic |
Las Palmas |
geographic_facet |
Las Palmas |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
Sí 3rd International Symposium on Fish & Shellfish Immunology (2019) http://hdl.handle.net/10261/203745 |
op_rights |
none |
_version_ |
1790607791530967040 |