Bioactive hydrolysate from alginate extraction by-product of Laminaria digitata. Encapsulation in liposomes and stabilization methods

• Main Authors: Montero García, Pilar, Pérez García, Alba, Alemán, Ailén, Gómez Estaca, Joaquín, Sánchez-Guisado, Mª Jesús, Fernández de Palencia, P., Gómez Guillén, M. C.
• Other Authors: Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission
• Format: Still Image
• Language: English
• Published: 2019
• Subjects: Faroe Islands; Tórshavn
• Online Access: http://hdl.handle.net/10261/203500; https://doi.org/10.13039/501100011033; https://doi.org/10.13039/501100003329; https://doi.org/10.13039/501100000780

Trabajo presentado a la 49th West European Fish Technologists Association (WEFTA) Conference celebrada en Tórshavn (Faroe Islands) del 15 al 17 de octubre de 2019. Although the consumption of algae in the western diet is increasingly growing, the main use of these is to obtain their polymers, which in the case of brown algae, such as Laminaria, are mainly alginates, fucoidan and laminaran. During the extraction process of polysaccharides, other compounds are usually removed, which constitute a bioactive potential of great interest, as they are usually proteins, polyphenols and carotenoids in variable proportion according to species. The OBJECTIVE of this work is the integral use of Laminaria digitata, through the extraction of alginate, and the by-products generated, such a proteins, which are extracted as hydrolysates. The hydrolysate encapsulation in nanoliposomes of phosphatidylcholine and their stabilization with sodium alginate by means of drying methods (atomization and lyophilization) is also intended, as a model of application. Sodium alginate was extracted from Laminaria digitata with a yield of 66%. The by-products were used to obtain a hydrolysate with antioxidant and antihypertensive properties, which could be encapsulated with high encapsulation efficiency in soy phosphatidylcholine liposomes. The cellular viability of the Laminaria hydrolysate and the liposome loaded with hydrolysate in CaCo 2 and THP 1 cell lines was 100% in all the tested concentrations. These liposomes were stabilized by coating with the extracted sodium alginate by using one of the two processes: lyophilization or atomization. The atomization proved to be more suitable for desiccation and preservation of the liposomal particle properties. Of the coated liposomes, the atomized ones were slightly smaller than the lyophilized ones. Both liposomes showed high stability at different pH during one week stored in refrigeration, with very electronegative Z potential values. This work has been financed by the Spanish MINECO, ...