Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach
8 pages, 4 figures, 1 table The usefulness of a molecular approach based on polymerase chain reaction (PCR) was investigated to identify and quantify the feeding of larval krill on zooplankton organisms in the Lazarev Sea during winter in 2006. Different primers and probes of dominant copepod specie...
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Online Access: | http://hdl.handle.net/10261/20272 https://doi.org/10.1007/s00300-009-0714-2 |
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ftcsic:oai:digital.csic.es:10261/20272 2024-02-11T10:03:36+01:00 Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach Töbe, Kerstin Meyer, Bettina Fuentes, Veronica 2009-09-12 5875 bytes application/pdf http://hdl.handle.net/10261/20272 https://doi.org/10.1007/s00300-009-0714-2 en eng Springer https://doi.org/10.1007/s00300-009-0714-2 Polar Biology 33(3): 407-414 (2009) 0722-4060 http://hdl.handle.net/10261/20272 doi:10.1007/s00300-009-0714-2 1432-2056 none Krill larvae Copepod prey PCR Quantitative PCR artículo http://purl.org/coar/resource_type/c_6501 2009 ftcsic https://doi.org/10.1007/s00300-009-0714-2 2024-01-16T09:25:09Z 8 pages, 4 figures, 1 table The usefulness of a molecular approach based on polymerase chain reaction (PCR) was investigated to identify and quantify the feeding of larval krill on zooplankton organisms in the Lazarev Sea during winter in 2006. Different primers and probes of dominant copepod species (Oithona sp., Ctenocalanus citer, copepodid stages of Metridia gerlachei and Calanoides acutus), co-occurring with larval krill under sea ice during winter, were developed for quantitative PCR (qPCR) and their species specificity was tested on target and non-target species. The qPCR results showed that larval krill were exclusively feeding on Oithona sp. This result was confirmed by microscopic analysis of stomach and gut contents of larvae from the same stations. This work was supported by funding from the German Ministry of Education and Science through project 03F0400A, Subproject 4 of the Lazarev Sea Krill Study (LAKRIS) Project. The LAKRIS-Project is the German contribution to the Southern Ocean-Global Ocean Ecosystem Dynamics (SO-GLOBEC) programme. Peer reviewed Article in Journal/Newspaper Euphausia superba Lazarev Sea Polar Biology Sea ice Southern Ocean Digital.CSIC (Spanish National Research Council) Lazarev ENVELOPE(12.917,12.917,-69.967,-69.967) Lazarev Sea ENVELOPE(7.000,7.000,-68.000,-68.000) Southern Ocean Polar Biology 33 3 407 414 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Krill larvae Copepod prey PCR Quantitative PCR |
spellingShingle |
Krill larvae Copepod prey PCR Quantitative PCR Töbe, Kerstin Meyer, Bettina Fuentes, Veronica Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach |
topic_facet |
Krill larvae Copepod prey PCR Quantitative PCR |
description |
8 pages, 4 figures, 1 table The usefulness of a molecular approach based on polymerase chain reaction (PCR) was investigated to identify and quantify the feeding of larval krill on zooplankton organisms in the Lazarev Sea during winter in 2006. Different primers and probes of dominant copepod species (Oithona sp., Ctenocalanus citer, copepodid stages of Metridia gerlachei and Calanoides acutus), co-occurring with larval krill under sea ice during winter, were developed for quantitative PCR (qPCR) and their species specificity was tested on target and non-target species. The qPCR results showed that larval krill were exclusively feeding on Oithona sp. This result was confirmed by microscopic analysis of stomach and gut contents of larvae from the same stations. This work was supported by funding from the German Ministry of Education and Science through project 03F0400A, Subproject 4 of the Lazarev Sea Krill Study (LAKRIS) Project. The LAKRIS-Project is the German contribution to the Southern Ocean-Global Ocean Ecosystem Dynamics (SO-GLOBEC) programme. Peer reviewed |
format |
Article in Journal/Newspaper |
author |
Töbe, Kerstin Meyer, Bettina Fuentes, Veronica |
author_facet |
Töbe, Kerstin Meyer, Bettina Fuentes, Veronica |
author_sort |
Töbe, Kerstin |
title |
Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach |
title_short |
Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach |
title_full |
Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach |
title_fullStr |
Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach |
title_full_unstemmed |
Detection of zooplankton items in the stomach and gut content of larval krill, Euphausia superba, using a molecular approach |
title_sort |
detection of zooplankton items in the stomach and gut content of larval krill, euphausia superba, using a molecular approach |
publisher |
Springer |
publishDate |
2009 |
url |
http://hdl.handle.net/10261/20272 https://doi.org/10.1007/s00300-009-0714-2 |
long_lat |
ENVELOPE(12.917,12.917,-69.967,-69.967) ENVELOPE(7.000,7.000,-68.000,-68.000) |
geographic |
Lazarev Lazarev Sea Southern Ocean |
geographic_facet |
Lazarev Lazarev Sea Southern Ocean |
genre |
Euphausia superba Lazarev Sea Polar Biology Sea ice Southern Ocean |
genre_facet |
Euphausia superba Lazarev Sea Polar Biology Sea ice Southern Ocean |
op_relation |
https://doi.org/10.1007/s00300-009-0714-2 Polar Biology 33(3): 407-414 (2009) 0722-4060 http://hdl.handle.net/10261/20272 doi:10.1007/s00300-009-0714-2 1432-2056 |
op_rights |
none |
op_doi |
https://doi.org/10.1007/s00300-009-0714-2 |
container_title |
Polar Biology |
container_volume |
33 |
container_issue |
3 |
container_start_page |
407 |
op_container_end_page |
414 |
_version_ |
1790599888908582912 |