Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae?
17 pages, 3 tables, 4 figures Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid in...
Published in: | Journal of Invertebrate Pathology |
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Online Access: | http://hdl.handle.net/10261/198658 https://doi.org/10.1016/j.jip.2019.107259 |
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ftcsic:oai:digital.csic.es:10261/198658 2024-02-11T10:03:10+01:00 Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae? Fernández-Boo, Sergio Gervais, Ophélie Prado-Álvarez, María Chollet, Bruno Claverol, Stéphane Lecadet, Cyrielle Dubreuil, Christine Arzul, Isabelle 2020 http://hdl.handle.net/10261/198658 https://doi.org/10.1016/j.jip.2019.107259 en eng Elsevier Postprint https://doi.org/10.1016/j.jip.2019.107259 Sí Journal of Invertebrate Pathology 169: 107259 (2020) 0022-2011 http://hdl.handle.net/10261/198658 doi:10.1016/j.jip.2019.107259 1096-0805 open Bonamia ostreae Crassostrea gigas Ostrea edulis Flow cytometry Immune response Proteome artículo http://purl.org/coar/resource_type/c_6501 2020 ftcsic https://doi.org/10.1016/j.jip.2019.107259 2024-01-16T10:47:28Z 17 pages, 3 tables, 4 figures Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to better understand the potential role of mucus in the defense of the oyster against parasites such as B. ostreae, liquid chromatography and tandem mass spectrometry were used to describe and compare mucus protein composition from both species. In all oyster species, pallial mucus contains a high level of proteins; however, O. edulis mucus produced a variety of proteins that could be involved in the immune response against the parasite, including Cu/Zn extracellular superoxide dismutase, thioxiredoxin, peroxiredon VI, heat shock protein 90 as well as several hydrolases. Conversely, a different set of antioxidant proteins, hydrolases and stress related proteins were identified in mucus from C. gigas. Our results suggest an innate immunity adaptation of oysters to develop a specific response against their respective pathogens. The mucosal protein composition also provides new insights for further investigations into the immune response in oysters The Région of Poitou Charentes and IFREMER supported this research. This work was also supported by the project POCI-01-0145-FEDER-030232, co-financed by COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by the FCT through national funds and strategical project ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Digital.CSIC (Spanish National Research Council) Pacific Journal of Invertebrate Pathology 169 107259 |
institution |
Open Polar |
collection |
Digital.CSIC (Spanish National Research Council) |
op_collection_id |
ftcsic |
language |
English |
topic |
Bonamia ostreae Crassostrea gigas Ostrea edulis Flow cytometry Immune response Proteome |
spellingShingle |
Bonamia ostreae Crassostrea gigas Ostrea edulis Flow cytometry Immune response Proteome Fernández-Boo, Sergio Gervais, Ophélie Prado-Álvarez, María Chollet, Bruno Claverol, Stéphane Lecadet, Cyrielle Dubreuil, Christine Arzul, Isabelle Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae? |
topic_facet |
Bonamia ostreae Crassostrea gigas Ostrea edulis Flow cytometry Immune response Proteome |
description |
17 pages, 3 tables, 4 figures Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to better understand the potential role of mucus in the defense of the oyster against parasites such as B. ostreae, liquid chromatography and tandem mass spectrometry were used to describe and compare mucus protein composition from both species. In all oyster species, pallial mucus contains a high level of proteins; however, O. edulis mucus produced a variety of proteins that could be involved in the immune response against the parasite, including Cu/Zn extracellular superoxide dismutase, thioxiredoxin, peroxiredon VI, heat shock protein 90 as well as several hydrolases. Conversely, a different set of antioxidant proteins, hydrolases and stress related proteins were identified in mucus from C. gigas. Our results suggest an innate immunity adaptation of oysters to develop a specific response against their respective pathogens. The mucosal protein composition also provides new insights for further investigations into the immune response in oysters The Région of Poitou Charentes and IFREMER supported this research. This work was also supported by the project POCI-01-0145-FEDER-030232, co-financed by COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by the FCT through national funds and strategical project ... |
format |
Article in Journal/Newspaper |
author |
Fernández-Boo, Sergio Gervais, Ophélie Prado-Álvarez, María Chollet, Bruno Claverol, Stéphane Lecadet, Cyrielle Dubreuil, Christine Arzul, Isabelle |
author_facet |
Fernández-Boo, Sergio Gervais, Ophélie Prado-Álvarez, María Chollet, Bruno Claverol, Stéphane Lecadet, Cyrielle Dubreuil, Christine Arzul, Isabelle |
author_sort |
Fernández-Boo, Sergio |
title |
Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae? |
title_short |
Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae? |
title_full |
Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae? |
title_fullStr |
Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae? |
title_full_unstemmed |
Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae? |
title_sort |
is pallial mucus involved in ostrea edulis defenses against the parasite bonamia ostreae? |
publisher |
Elsevier |
publishDate |
2020 |
url |
http://hdl.handle.net/10261/198658 https://doi.org/10.1016/j.jip.2019.107259 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
Postprint https://doi.org/10.1016/j.jip.2019.107259 Sí Journal of Invertebrate Pathology 169: 107259 (2020) 0022-2011 http://hdl.handle.net/10261/198658 doi:10.1016/j.jip.2019.107259 1096-0805 |
op_rights |
open |
op_doi |
https://doi.org/10.1016/j.jip.2019.107259 |
container_title |
Journal of Invertebrate Pathology |
container_volume |
169 |
container_start_page |
107259 |
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1790599345229266944 |