Building Tara Oceans Polar Circle Metagenomic Assembled Genomes (MAGs)

• Main Authors: Sánchez, Pablo, Royo-Llonch, Marta, Pedrós-Alió, Carlos, Tara Oceans Consortium, Acinas, Silvia G.
• Format: Conference Object
• Language: unknown
• Published: Scientific Committee on Antarctic Research 2018
• Subjects: Tara Oceans Polar Circle; Bioinformatics; Metagenomics; MAGs; Prokaryotes; Antarctic; Arctic; Arctic Ocean; Antarc*; SCAR; Scientific Committee on Antarctic Research
• Online Access: http://hdl.handle.net/10261/193265

IX Simposio de Estudios Polares del Comité Español del Scientific Committee on Antarctic Research (SCAR), 5-7 September 2018, Madrid, España.-- 1 page In 2013 Tara Oceans navigated Arctic waters during spring, summer and the beginning of fall (May to October), extensively sampling the microbial plankton communities. Although there are several seminal studies looking at microbial diversity and community structure in specific regions of the Arctic Ocean by 16S High Throughput Sequencing there is still a gap in the link between diversity with functional capacities of microbial species. Here, we show the bioinformatics strategy that has enabled the reconstruction of 2555 bacterial and archaeal Metagenomic Assembled Genomes (MAGs) from 41 microbial metagenomes, covering the whole Arctic Ocean from spring to early fall at different water depths. Of the reconstructed prokaryotic MAGs, 96 are considered to be high-quality draft-like genomes (>90% genome completeness, <5% contamination) and 526 are considered of medium quality (>50% genome completeness, <10% contamination). This Tara Oceans Polar MAGs dataset represents the first attempt to link genetics with functional and metabolic capacities of prokaryotes, which together with the physicochemical information of all stations will allow us to unveil the keystone bacterial and archaeal in the Arctic Ocean at the maximum resolution possible, the genome level Peer Reviewed